Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Production of monoclonal antibodies against infectious hematopoietic necrosis virus (IHNV)

Infectious hematopoietic necrosis virus (IHNV)에 대한 단클론 항체 생산

  • Kyoung-Hui Kong (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Myung-Joo Oh (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University) ;
  • Choon-Sup Kim (Enbiogene) ;
  • Wi-Sik Kim (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
  • 공경희 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과) ;
  • 김춘섭 ((주)엔바이로젠) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Received : 2023.11.14
  • Accepted : 2023.12.01
  • Published : 2023.12.31
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Abstract

Infectious hematopoietic necrosis virus (IHNV) is s significant viral pathogen affecting cultured rainbow trout (Oncorhynchus mykiss) in Korea. In this study, five monoclonal antibodies (mAbs) (IHNV-1, 2, 3, 4, and 5) were produced using purified IHNV. Reactivities of these mAbs were analyzed by western blot (WB), enzyme-linked immunosorbent assay (ELISA), and indirect fluorescent antibody test (IFAT). These mAbs recognized glycoprotein (69 kDa, IHNV-1), nucleocapsid protein (39 kDa, IHNV-3, 4, and 5), or phosphoprotein (27 kDa, IHNV-2) of IHNV by WB analysis. ELISA results indicated that these five mAbs were specific to IHNV without showing any cross-reactivity against other fish viruses (hirame rhabdovirus, infectious pancreatic necrosis virus, and viral hemorrhagic septicemia virus). IFAT demonstrated specific fluorescence signals of IHNV-infected epithelioma papulosum cyprini (EPC) cells, whereas no reactivity of normal EPC cells was observed. These mAbs can be very useful for immuno-diagnosis of IHNV infection.

Keywords

Acknowledgement

이 논문은 2023년 전라남도와 (재)전남테크노파크의 지역수요맞춤형 연구개발사업의 지원을 받아 수행된 연구임.

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