공업화학 (Applied Chemistry for Engineering)

  • 제34권6호
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  • Pages.660-664
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  • 2023
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  • 1225-0112(pISSN)
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  • 2288-4505(eISSN)
한국공업화학회 (The Korean Society of Industrial and Engineering Chemistry)
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Chromatic Detection of Cholesterol Using Polydiacetylene Vesicles

  • Min Jae Shin (Department of Chemical and Biological Engineering, Andong National University)
  • 투고 : 2023.10.30
  • 심사 : 2023.11.14
  • 발행 : 2023.12.10
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초록

In this study, a new system to determine the concentration of cholesterol using a color change was developed. The system comprised diacetylene vesicles and cholesterol oxidase (ChOx). 10,12-Pentacosadiynoic acid (PCDA) was used as the diacetylene compound, and PCDA vesicles were formed using sonication. The H2O2 produced during the reaction between cholesterol and ChOx was used to initiate the polymerization of the PCDA in the vesicles. During polymerization, the vesicles changed from colorless to blue. Therefore, the cholesterol concentration was proportional to the intensity of the blue color. The absorption at 665 nm indicated that the blue color was directly proportional to the cholesterol concentration. This indicates that the system can be used for cholesterol detection. The minimum cholesterol concentration detected using this system was 1.0 mM.

키워드

참고문헌

  1. H. M. Yadav, J. D. Park, H. C. Kang, and J. J. Lee, Recent development in nanomaterial-based electrochemical sensors for cholesterol detection, Chemosensors, 9, 98 (2021). 
  2. H. Song, H. Shin, H. Seo, W. Park, B. J. Joo, J. Kim, H. K. Kim, J. Kim, and J. U. Park, Wireless non-invasive monitoring of cholesterol using a smart contact lens, Adv. Sci., 9, 2203597 (2022). 
  3. P. T. Nguyen, Y. I. Kim, and M. I. Kim, Reagent-free colorimetric cholesterol test strip based on self color-changing property of nanoceria, Front. Chem., 8, 798 (2020). 
  4. T. Ahmadraji and A. J. Killard, Measurement of total cholesterol using an enzyme sensor based on a printed hydrogen peroxide electrocatalyst, Anal. Methods, 8, 2743-2749 (2016).  https://doi.org/10.1039/C6AY00468G
  5. A. N. Sekretaryova, V. Beni, M. Eriksson, A. A. Karyakin, A. P. F. Turner, and M. Y. Vagin, Cholesterol self-powered biosensor, Anal. Chem., 86, 9540-9547 (2014).  https://doi.org/10.1021/ac501699p
  6. L. Hong, A. L. Liu, G. W. Li, W. Chen, and X. H. Lin, Chemiluminescent cholesterol sensor based on peroxidase-like activity of cupric oxide nanoparticles, Biosens. Bioelectron., 43, 1-5 (2013).  https://doi.org/10.1016/j.bios.2012.11.031
  7. A. A. Ansari, A. Kaushik, P. R. Solanki, and B. D. Malhotra, Electrochemical cholesterol sensor based on tin oxide-chitosan nanobiocomposite film, Electroanalysis, 21, 965-972 (2009).  https://doi.org/10.1002/elan.200804499
  8. S. Veeralingam and S. Badhulika, Enzyme immobilized multiwalled carbon nanotubes on paper-based biosensor fabricated via mask-less hydrophilic and hydrophobic microchannels for cholesterol detection, J. Ind. Eng. Chem., 113, 401-410 (2022).  https://doi.org/10.1016/j.jiec.2022.06.015
  9. M. Ahmad, A. Nisar, and H. Sun, Emerging trends in non-enzymatic cholesterol biosensors: Challenges and advancements, Biosensors, 12, 955 (2022). 
  10. N. Thakur, D. Mandal, and T. C. Nagaiah, A novel NiVP/Pi-based flexible sensor for direct electrochemical ultrasensitive detection of cholesterol, Chem. Commun., 58, 2706-2709 (2022).  https://doi.org/10.1039/D1CC07115G
  11. V. Joshi, S. Hussain, S. Dua, N. Arora, S. H. Mir, G. Rydzek, and T. Senthilkumar, Oligomer sensor nanoarchitectonics for "turn-on" fluorescence detection of cholesterol at the nanomolar level, Molecules, 27, 2856 (2022). 
  12. N. Jahani, M. Amiri, M. Ghiasi, H. Imanzadeh, R. Boukherroub, S. Szunerits, F. Marken, and N. B. McKeown, Non-enzymatic electrochemical cholesterol sensor based on strong host-guest interactions with a polymer of intrinsic microporosity (PIM) with DFT study, Anal. Bioanal. Chem., 413, 6523-6533 (2021).  https://doi.org/10.1007/s00216-021-03616-w
  13. A. Nagarajan, V. Sethuraman, T. M. Sridhar, and R. Sasikumar, Development of Au@NiO decorated polypyrrole composite for non-Enzymatic electrochemical sensing of cholesterol, J. Ind. Eng. Chem., 120, 460-466 (2023).  https://doi.org/10.1016/j.jiec.2022.12.054
  14. M. A. Hefnawy, S. A. Fadlallah, R. M. El-Sherif, and S. S. Medany, Competition between enzymatic and non-enzymatic electrochemical determination of cholesterol, J. Electroanal. Chem., 930, 117169 (2023). 
  15. D. Feng, P. Huang, Y. Miao, A. Liang, X. Wang, B. Tang, H. Hou, M. Ren, S. Gao, L. Geng, and A. Luo, Novel photoelectrochemical sensor for cholesterol based on CH3NH3PbBr3 perovskite/TiO2 inverse opal heterojunction coated with molecularly imprinted polymers, Sens. Actuators B Chem., 368, 132121 (2022). 
  16. M. J. Shin, M. Kim, and J. S. Shin, Switchable cholesterol recognition system with Diels-Alder reaction using molecular imprinting technique on self-assembled monolayer, Polym. Int., 68, 1722-1728 (2019).  https://doi.org/10.1002/pi.5877
  17. M. J. Shin, Y. J. Shin, and J. S. Shin, Sensing capability of molecularly imprinted self-assembled monolayer fabricated using dithiol compound, J. Ind. Eng. Chem., 20, 91-95 (2014).  https://doi.org/10.1016/j.jiec.2013.04.022
  18. M. J. Shin, Y. J. Shin, and J. S. Shin, Cholesterol recognition system by molecular imprinting on self-assembled monolayer, Colloid Surf. A, 559, 365=371 (2018).
  19. B. Das, S. Jo, J. Zheng, J. Chen, and K. Sugihara, Recent progress in polydiacetylene mechanochromism, Nanoscale, 14, 1670-1678 (2022).  https://doi.org/10.1039/D1NR07129G
  20. X. Qian and B. Stadler, Recent developments in polydiacetylene-based sensors, Chem. Mater., 31, 1196-1222 (2019).  https://doi.org/10.1021/acs.chemmater.8b05185
  21. H. Kim and M. J. Shin, Electrospun coaxial microfiber-based water detecting sensor using expansion pressure mechanism, Polym. Eng. Sci., 63, 167-175 (2023).  https://doi.org/10.1002/pen.26195
  22. M. J. Shin and J. S. Shin, A molecularly imprinted polymer undergoing a color change depending on the concentration of bisphenol A, Microchimi. Acta, 187, 44 (2020). 
  23. M. J. Shin and J. S. Shin, Chromatic response of cationic polydiacetylene vesicles induced by permeation of target compound, J. Appl. Polym. Sci., 137, 49355 (2020). 
  24. M. J. Shin and J. S. Shin, Effect of ethanol on the color transition of the polydiacetylene vesicle of 10,12-pentacosadiynoic acid for butylamine detection, J. Appl. Polym. Sci., 136, 47688 (2019). 
  25. M. J. Shin, Detection of neomycin using amino-functionalized polydiacetylene, Macromol. Res., 28, 703-708 (2020).  https://doi.org/10.1007/s13233-020-8088-y
  26. M. Kim, Y. J. Shin, S. W. Hwang, M. J. Shin, and J. S. Shin, Chromatic detection of glucose using polymerization of diacetylene vesicle, J. Appl. Polym. Sci., 135, 46394 (2018).