The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Study on Chondro-protective and Anti-inflammatory Effects of Caraganae Sinicae Flos Extract

골담초꽃 추출물의 연골 보호 및 염증 억제 효과에 관한 연구

  • 박동준 (LG생활건강 기술연구원) ;
  • 이홍구 (LG생활건강 기술연구원) ;
  • 고충호 (국립수목원 정원식물자원과) ;
  • 박형국 (LG생활건강 기술연구원) ;
  • 진무현 (LG생활건강 기술연구원) ;
  • 조호성 (LG생활건강 기술연구원)
  • Received : 2022.09.16
  • Accepted : 2022.11.25
  • Published : 2022.11.30
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Abstract

Objectives : This research aimed to investigate chondro-protective and anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract and its compound, tilianin. Methods : Caraganae Sinicae Flos was extracted with 50% ethanol. Tilianin in Caraganae Sinicae Flos 50% ethanol extract was quantified by HPLC analysis method. To investigate chondro-protective effects of Caraganae Sinicae Flos 50% ethanol extract, ATDC5 chondrogenic cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and tumor necrosis factor-𝛼 (TNF𝛼) for 24 hours. After treatement for 24 hours, media supernatant was used for quantifying protein level of matrix metalloproteinase-3 (MMP3) by ELISA and harvested cells were used for analyzing mRNA expression level of matrix metalloproteinase-13 (MMP13) by reverse transcription PCR. To identify anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract, RAW 264.7 macrophage cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and lipopolysaccharide (LPS) for 24 hours. media was used for quantifying the level of prostaglandin E2 (PGE2), interleukin-6 (IL6) by ELISA and nitric oxide by Griess reagent asssay. Results : Caraganae Sinicae Flos 50% ethanol extract and tilianin attenuated protein level of MMP3 and mRNA expression level of MMP13 in TNF𝛼-activated ATDC5 cells. Caraganae Sinicae Flos 50% ethanol extract inhibited the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, though tilianin inhibited PGE2 only. Conclusions : These results presented that Caraganae Sinicae Flos 50% ethanol extract could be used as natural medicines for osteoarthritis.

Keywords

References

  1. Zhang H, Wang H, Zeng C, Yan B, Ouyang J, Liu X, et al. mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis. Osteoarthritis Cartilage. 2017 ; 25(6) : 952-63. https://doi.org/10.1016/j.joca.2016.12.024
  2. Robinson WH, Lepus CM, Wang Q, Raghu H, Mao R, Lindstrom TM, Sokolove J. Low-grade inflammation as a key mediator of the pathogenesis of osteoarthritis. Nat Rev Rheumatol. 2016 ; 12(10) : 580-92. https://doi.org/10.1038/nrrheum.2016.136
  3. Donell S. Subchondral bone remodelling in osteoarthritis. EFORT Open Rev. 2019 ; 4(6) : 221-29. https://doi.org/10.1302/2058-5241.4.180102
  4. Lu J, Zhang H, Cai D, Zeng C, Lai P, Shao Y, et al. Positive feedback regulation of subchondral H-type vessel formation by chondrocyte promotes osteoarthritis development in mice. J Bone Miner Res. 2018 ; 33 : 909-20. https://doi.org/10.1002/jbmr.3388
  5. Cui Z, Crane J, Xie H, Jin X, Zhen G, Li C, et al. Halofuginone attenuates osteoarthritis by inhibition of TGF-beta activity and H-type vessel formation in subchondral bone. Ann Rheum Dis. 2016 ; 75 : 1714-21. https://doi.org/10.1136/annrheumdis-2015-207923
  6. Hugle T, Geurts J. What drives osteoarthritis - synovial versus subchondral bone pathology. Rheumatology. 2017 ; 56(9) : 1461-71. https://doi.org/10.1093/rheumatology/kew389
  7. Kim TY, Kim JH, Park JH, Lee JK, Han C, Kum CJ, Oh JW. The Domestic Trends of Traditional Korean Medicine Treatments on Degenerative Knee Arthritis. Journal of Korea CHUNA Manual Medicine. 2014 ; 9(2) : 69-79.
  8. Jang JH, Kim KH. A Study on Osteoarthritis of the Knee. J Acupunct Res. 1998 ; 15(2) : 493-509.
  9. Park YK. Inhibitory effects of Zanthoxylum piperitum on the LPS-induced production of nitric oxide and proinflammatory cytokines in RAW264.7 cells. Kor. J. Herbol. 2006 ; 21(4) : 69-76.
  10. Bae GC, Bae DY. The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea. Kor. J. Herbol. 2012 ; 27(6) : 55-61. https://doi.org/10.6116/KJH.2012.27.6.55
  11. Yoo JH, Doh ES, Chang JP, Kil KJ. Antioxidant Activities of Hot Water and Ethanol Extracts from the stem of Sorbus commixta Heal. Kor. J. Herbol. 2017；32(3):29-36. https://doi.org/10.6116/KJH.2017.32.3.29
  12. Chae YA, et al. Medicinal plant biology. Seoul : Hyangmunsa CO. 2007 : 1.
  13. KIM TJ. Korean resources plants II. Seoul : Seoul National University press. 1997 : 11.
  14. Ma CW, Ham I, Whang WK. The flavonoids from the Caragana chamlagu leaves. Yakhak Hoechi. 1999 ; 43(2) : 143-9.
  15. Zhang LP, Ju MH, Hu CQ. Studies on isoflavones from Chinese peashrub (Caragana sinica). Chinese Traditional Herbal Drugs. 1994 ; 24 : 134-6.
  16. Qi JB, Shu N, Ma DY, Hu CQ. Isoflavonoid compounds from roots of Caragana sinica. Zhongguo Tianran Yaowu. 2007 ; 5 : 101-4.
  17. Wang S, Ma D, Hu C. Three new compounds from the aerial parts of Caragana sinica. Helvetica Chimica Acta. 2005 ; 88 : 2315-21. https://doi.org/10.1002/hlca.200590166
  18. Sokolove J, Lepus CM. Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis. 2013 ; 5(2) : 77-94. https://doi.org/10.1177/1759720X12467868
  19. Klatt AR, Paul-Klausch B, Klinger G, Kuhn G, Renno JH, Banerjee M, Malchau G, Wielckens K. A critical role for collagen II in cartilage matrix degradation: collagen II induces pro-inflammatory cytokines and MMPs in primary human chondrocytes. J Orthop Res. 2009 ; 27 : 65-70. https://doi.org/10.1002/jor.20716
  20. Lambert C, Borderie D, Dubuc JE, Rannou F, Henrotin Y. Type II collagen peptide Coll2-1 is an actor of synovitis. Osteoarthr Cartil. 2019 ; 27 : 1680-91. https://doi.org/10.1016/j.joca.2019.07.009
  21. Lees S, Golub SB, Last K, Zeng W, Jackson DC, Sutton P, Fosang AJ. Bioactivity in an Aggrecan 32-mer fragment is mediated via toll-like receptor 2. Arthritis Rheumatol. 2015 ; 67(5) : 1240-9. https://doi.org/10.1002/art.39063
  22. Rubartelli A, Lotze MT. Inside, outside, upside down: damage-associated molecular-pattern molecules (DAMPs) and redox. Trends immunol. 2007 ; 28(10) : 429-36. https://doi.org/10.1016/j.it.2007.08.004
  23. Zhang H, Cai D, Bai X. Macrophages regulate the progression of osteoarthritis. Osteoarthritis Cartilage. 2020 ; 28(5) : 555-61. https://doi.org/10.1016/j.joca.2020.01.007
  24. Latourte A, Cherifi C, Maillet J, Ea HK, Bouaziz W, Funck-Brentano T, Cohen-Solal M, Hay E, Richette P. Systemic inhibition of IL-6/Stat3 signalling protects against experimental osteoarthritis. Ann Rheum Dis. 2017 ; 76(4) : 748-55. https://doi.org/10.1136/annrheumdis-2016-209757
  25. Zhang Y, Fu Z. Role of tilianin against acute lung injury in In vitro LPS-induced alveolar macrophage cells and an in vivo C57BL/6 mice model. J Environ Pathol Toxicol Oncol. 2020 ; 39(4) : 335-44. https://doi.org/10.1615/jenvironpatholtoxicoloncol.2020035136
  26. Li T, Zou QP, Huang F, Cheng GG, Mao ZW, Wang T, Dong FW, Li BJ, He HP, Li YP. Flower extract of Caragana sinica. ameliorates DSS-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model. Iran J Basic Med Sci. 2021 ; 24(5) : 595-603.
  27. Jeon YS, Jo BS, Park HJ, Kang SA, Cho YJ. Screening of Biological Activity of Caragana sinica Extracts. J Korean Soc Food Sci Nutr. 2012 ; 41(9) : 1211-9. https://doi.org/10.3746/JKFN.2012.41.9.1211