Journal of Microbiology and Biotechnology

  • 제32권10호
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  • Pages.1344-1354
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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KIF26B-AS1 Regulates TLR4 and Activates the TLR4 Signaling Pathway to Promote Malignant Progression of Laryngeal Cancer

  • Li, Li (Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Lianyungang City) ;
  • Han, Jiahui (Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Lianyungang City) ;
  • Zhang, Shujia (Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Lianyungang City) ;
  • Dong, Chunguang (Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Lianyungang City) ;
  • Xiao, Xiang (Department of Otolaryngology, Head and Neck Surgery, The First People's Hospital of Lianyungang City)
  • 투고 : 2022.03.21
  • 심사 : 2022.07.04
  • 발행 : 2022.10.28
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초록

Laryngeal cancer is one of the highest incidence, most prevalently diagnosed head and neck cancers, making it critically necessary to probe effective targets for laryngeal cancer treatment. Here, real-time quantitative reverse transcription PCR (qRT-PCR) and western blot analysis were used to detect gene expression levels in laryngeal cancer cell lines. Fluorescence in situ hybridization (FISH) and subcellular fractionation assays were used to detect the subcellular location. Functional assays encompassing Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), transwell and wound healing assays were performed to examine the effects of target genes on cell proliferation and migration in laryngeal cancer. The in vivo effects were proved by animal experiments. RNA-binding protein immunoprecipitation (RIP), RNA pulldown and luciferase reporter assays were used to investigate the underlying regulatory mechanisms. The results showed that KIF26B antisense RNA 1 (KIF26B-AS1) propels cell proliferation and migration in laryngeal cancer and regulates the toll-like receptor 4 (TLR4) signaling pathway. KIF26B-AS1 also recruits FUS to stabilize TLR4 mRNA, consequently activating the TLR4 signaling pathway. Furthermore, KIF26B-AS1 plays an oncogenic role in laryngeal cancer via upregulating TLR4 expression as well as the FUS/TLR4 pathway axis, findings which offer novel insight for targeted therapies in the treatment of laryngeal cancer patients.

키워드

과제정보

We thank all our experimenters for their efforts. This research did not receive any specific funding from agencies in the public, commercial, or not-for-profit sectors.

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