Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Increased Antioxidants of Agastache rugosa by the Night Interruption Time

야파(night interruption)처리에 의한 배초향의 항산화 물질 증가

  • Kim, Sungjin (Department of Horticultural Science, Chungnam National University) ;
  • Noh, Seungwon (Department of Bio-AI Convergence, Chungnam National University) ;
  • Park, Jongseok (Department of Horticultural Science, Chungnam National University)
  • 김성진 (충남대학교 원예학과) ;
  • 노승원 (충남대학교 바이오 AI 융합학과) ;
  • 박종석 (충남대학교 농업생명과학대학 원예학과)
  • Received : 2022.09.06
  • Accepted : 2022.10.10
  • Published : 2022.10.31
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Abstract

The objective of this study was to determine the proper night interruption of photoperiods and dark periods for accumulating bioactive compounds of Agastache rugosa without decreasing plant growth. Five-week-old seedlings were transplanted in a DFT system with white LEDs. A. rugosa was treated with night interruption time treatments of 18:1:2:3, 18:2:2:2, 18:3:2:1 (light:dark:light:dark), and 20:4 (control) for 4 weeks. There were no significant differences except for leaf length, leaf width, and the number of flowers. The content of antioxidants in the shoot of A. rugosa was high in tilianin and acacetin, and the content of rosmarinic acid (RA) was significantly higher in the underground part. The RA content per dry weight of A. rugosa was 47.92 and 51.46% higher than that of the control in 18:1:2:3 and 18:2:2:2, and tilianin and acactin per dry weight were significantly higher in 18:3:2:1. There was no significant difference in growth due to the same day light integral, but 18:2:2:2 showed high total polyphenol contents. Therefore, it is thought that the effect of increasing secondary metabolites of A. rugosa without degradation of growth can be expected through night interruption treatment in plant factory cultivation systems using artificial light.

본 연구는 식물 공장에서 배초향(Agastache rugosa Kuntze) 재배 시, 야파시간에 따른 생육과 항산화 물질 함량에 미치는 영향에 대하여 살펴보고자 수행하였다. 본엽 4매인 배초향 묘를 백색 LEDs를 이용한 수경재배 시스템에 정식하였다. 야파처리 효과를 알아보기 위해 06시부터 광도 200μmol·m-2·s-1 조건으로 시간을 24시간 주기에서 18:1:2:3, 18:2:2:2, 18:3:2:1(명기:암기:명기:암기) 비율로 처리하였으며 대조구는 20:4(명기:암기)로 설정하여 4주간 재배하였다. 엽장과 엽폭, 화아수를 제외하고는 유의적 차이가 발생하지 않았다. 배초향 지상부의 항산화물질 함량은 tilianin과 acacetin이 높았고, 지하부에서는 rosmarinic acid(RA)의 함량이 유의하게 높았다. 배초향의 건물중당 RA함량은 18:1:2:3과 18:2:2:2에서 대조구보다 47.92, 51.46% 높았고, 건물중당 tilianin과 acactin은 18:3:2:1에서 유의적으로 높게 나타났다. 야파처리를 하였을때 동일한 누적 광량으로 인해 생육 차이를 보이지 않았지만, 18:2:2:2의 야파처리가 총 폴리페놀 함량이 높게 나타났다. 따라서 인공광을 이용한 배초향 재배 시스템에서 야파처리를 통하여 생육 저하 없이 배초향의 항산화 물질 증대 효과를 기대할 수 있을 것으로 사료된다.

Keywords

Acknowledgement

본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 농업기반 및 재해대응 기술개발사업의 지원을 받아 연구되었음(322077-3).

References

  1. An J.U., K.H. Joung, H.S. Yoon, Y.H. Hwang, and G.P. Hong 2017, Effects of photo/dark period and relative humidity during dark period on growth and tipburn occurrence of water dropwort (Oenanthe stolonifera DC.) in a closed-type plant factory. Protected Hort Plant Fac 26:146-150. (in Korean) doi:/10.12791/KSBEC.2017.26.2.146
  2. Cha M.K., J.S. Kim, J.H. Shin, J.E. Son, and Y.Y. Cho 2014, Practical design of an artificial light-used plant factory for common ice plant (Mesembryanthemum crystallinum L.). Protected Hort Plant Fac 23:371-375. (in Korean) doi:10.12791/KSBEC.2014.23.4.371
  3. Dixon R.A., L. Achnine, P. Kota, C.J. Liu, M.S.S. Reddy, and L. Wang 2002, The phenylpropanoid pathway and plant defence a genomics perspective. Mol Plant Pathol 3:371-390. doi:10.1046/j.1364-3703.2002.00131.x
  4. Do J.W., S.W. Noh, G.J. Bok, H.J. Lee, J.W. Lee, and J.S. Park 2020, Selection of optimal varieties suitable for indoor cultivation considering the growth and functional content of Agastache species. Protected Hort Plant Fac 29:202-208. (in Korean) doi:10.12791/KSBEC.2020.29.2.202
  5. Dou H., G. Niu, M. Gu, and J.G. Masabni 2018, Responses of sweet basil to different daily light integrals in photosynthesis, morphology, yield, and nutritional quality. HortScience 53: 496-503. doi:10.21273/HORTSCI12785-17
  6. Engelsma G. 1978, Phenol synthesis and photomorphogenesis. Philips tech Rev 38:89-100.
  7. Engelsma G. 1979, Effect of daylength on phenol metabolism in the leaves of Salvia occidentalis. Plant Physiol 63:765-768. doi:10.1104/pp.63.4.765
  8. Han D.S., Y.C. Kim, S.E. Kim, H.S. Ju and S.J Byun 1987, Studies on the diterpene constituent of the root of Agastache rugosa O. Kuntze. J Pharmacogn 18:99-102. (in Korean)
  9. Hong Y.Y., Y.J. Park, Y.J. Kim, and K.S. Kim 2014, Vegetative growth and flowering of Salvia splendens 'Salsa' in response to night interruption. Hortic Sci Technol 32:434-439. (in Korean) doi:10.7235/hort.2014.13176
  10. Hwang H.S, H.W. Jeong, and S.J. Hwang 2022, Flowering and inflorescence development characteristics of Korean mint affected by photoperiod. J Bio-Env Con 31:188-193. doi: 10.12791/KSBEC.2022.31.3.188
  11. Jung D.H., H.I. Yoon, and J.E. Son 2017, Development of a three-variable canopy photosynthetic rate model of romaine lettuce (Lactuca sativa L.) grown in plant factory modules using light intensity, temperature, and growth stage. Protected Hort Plant Fac 26:268-275. (in Korean) doi:10.12791/KSBEC.2017.26.4.268
  12. Kim S.H., and J.H. Lieth 2003, A coupled model of photosynthesis, stomatal conductance and transpiration for a rose leaf (Rosa hybrida L.). Ann Bot 91:771-781. doi:10.1093/aob/mcg080
  13. Kim S.J., G.J. Bok, and J.S. Park 2018, Analysis of antioxidant content and growth of Agastache rugosa as affected by LED light qualities. Protected Hort Plant Fac 27:260-268. (in Korean) doi:10.12791/KSBEC.2018.27.3.260
  14. Kim Y.J., D.J. Yu, H. Rho, E.S. Runkle, H.J. Lee, and K.S. Kim 2015a, Photosynthetic changes in Cymbidium orchids grown under different intensities of night interruption lighting. Sci Hortic 186:124-128. doi:10.1016/j.scienta.2015.01.036
  15. Kim Y.J., Y.J. Park, and K.S. Kim 2015b, Night interruption promotes flowering and improves flower quality in Doritaenopsis orchid. Flower Res J 23:6-10. doi:10.11623/frj.2015.23.1.3
  16. Kim Y.J., T.K.L. Nguyen, and M.M. Oh 2020, Growth and ginsenosides content of ginseng sprouts according to LEDbased light quality changes. Agronomy 10:1979. doi:10.3390/agronomy10121979
  17. Kozai T. 2018, Smart Plant Factory: The Next Generation Indoor Vertical Farms, 1st ed. Springer, Berlin/Heidelberg, Germany, pp 3-14.
  18. Kwon D.Y., Y.B. Kim, J.K. Kim, and S.U. Park 2021, Production of rosmarinic acid and correlated gene expression in hairy root cultures of green and purple basil (Ocimum basilicum L.). Prep Biochem Biotechnol 51:35-43. doi:10.1080/10826068.2020.1789990
  19. Kwon Y.S., S.Y. Choi, M.J. Kil, B.S. You, J.A. Jung, and S.K. Park 2013, Effect of night break treatment using red LED (660 nm) on flower bud initiation and growth characteristics of chrysanthemum cv.'Baekma', and cv.'Jinba'. CNU J Agric Sci 40:297-303. (in Korean) doi:10.7744/cnujas.2013.40.4.297
  20. Lam V.P., S.J. Kim, H.J. Lee, and J.S. Park 2019, Root pruning increased bioactive compounds of hydroponically-grown Agastache rugosa in a greenhouse. Hortic Environ Biotechnol 60:647-657. doi:10.1007/s13580-019-00163-3
  21. Miyazawa Y., S. Hikosaka, E. Goto, and T. Aoki 2008, Effects of light conditions and air temperature on the growth of everbearing strawberry during the vegetative stage. Acta Hortic 842:817-820. doi:10.17660/ActaHortic.2009.842.180
  22. Oh D.G., M.K. Cha, and Y.Y. Cho 2017, Composition and EC of nutrient solution on growth and quality of carrot (Daucus carrota L.) in hydroponics. Protected Hort Plant Fac 26: 340-345. (in Korean) doi:10.12791/KSBEC.2017.26.4.340
  23. Park Y.J., Y.J. Kim, and K.S. Kim 2013, Vegetative growth and flowering of Dianthus, Zinnia, and Pelargonium as affected by night interruption at different timings. Hortic Environ Biotechnol 54:236-242. doi:10.1007/s13580-013-0012-3
  24. Petersen M. 1997, Cytochrome P450-dependent hydroxylation in the biosynthesis of rosmarinic acid in Coleus. Phytochemistry 45:1165-1172. doi:10.1016/S0031-9422(97)00135-0
  25. Rao S.R., and G.A. Ravishankar 2002, Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101-153. doi:10.1016/s0734-9750(02)00007-1
  26. Runkle E.S., and R.D. Heins 2006, Manipulating the light environment to control flowering and morphogenesis of herbaceous plants. Acta Hortic 711:51-60. doi:10.17660/ActaHortic.2006.711.4
  27. Shin S. 2004, Essential oil compounds from Agastache rugosa as antifungal agents against Trichophyton species. Arch Pharm Res 27:295-299. doi:10.1007/Bf02980063
  28. Sugumaran K.R., R.V. Sindhu, S. Sukanya, N. Aiswarya, and V. Ponnusami 2013, Statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed. Carbohydr Polym 98:854-860. doi:10.1016/j.carbpol.2013.06.071
  29. Sul S.G., Y.T. Baek, and Y.Y. Cho 2022, Effects of light intensity, light quality and photoperiod for growth of perilla in a closed-type plant factory system. J Bio-Env Con 31:180-187. (in Korean) doi:10.12791/KSBEC.2022.31.3.180
  30. Thomas B., and D. Vince-Prue 1997, Photoperiodism in plants. Academic Press, CA, USA.
  31. Trouwborst G., J. Oosterkamp, S.W. Hogewoning, J. Harbinson, and W. Van Ieperen 2010, The responses of light interception, photosynthesis and fruit yield of cucumber to LED-lighting within the canopy. Physiol Plant 138:289-300. doi:10.1111/j.1399-3054.2009.01333.x
  32. Tuan P.A., W.T. Park, H. Xu, N.I. Park, and S.U. Park 2012, Accumulation of tilianin and rosmarinic acid and expression of phenylpropanoid biosynthetic genes in Agastache rugosa. J Agric Food Chem 60:5945-5951. doi:10.1021/jf300833m
  33. Wang K.C., J.S. Chang, L.C. Chiang, and C.C. Lin 2009, 4-Methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line. Phytomedicine 16:882-886. doi:10.1016/j.phymed.2009.02.016
  34. Yadav V., Z. Wang, C. Wei, A. Amo, B. Ahmed, X. Yang, and X. Zhang 2020, Phenylpropanoid pathway engineering: An emerging approach towards plant defense. Pathogens 9:312. doi:10.3390/pathogens9040312
  35. Yeo H.J., C.H. Park, Y.E. Park, H. Hyeon, J.K. Kim, S.Y. Lee, and S.U. Park 2021, Metabolic profiling and antioxidant activity during flower development in Agastache rugosa. Physiol Mol Biol Plants 27:445-455. doi:10.1007/s12298-021-00945-z
  36. Zielinska S., and A. Matkowski 2014, Phytochemistry and bioactivity of aromatic and medicinal plants from the genus Agastache (Lamiaceae). Phytochem Rev 13:391-416. doi:10.1007/s11101-014-9349-1