Anatomy and Cell Biology

  • 제55권1호
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  • Pages.100-108
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  • 2022
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  • 2093-3665(pISSN)
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  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
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Evaluating the effect of conditioned medium from endometrial stem cells on endometriosis-derived endometrial stem cells

  • Seyedeh Saeideh Sahraei (Department of Mesenchymal Stem Cells, The Academic Centre for Education, Culture and Research) ;
  • Ali Kowsari (Department of Mesenchymal Stem Cells, The Academic Centre for Education, Culture and Research) ;
  • Faezeh Davoodi Asl (Department of Mesenchymal Stem Cells, The Academic Centre for Education, Culture and Research) ;
  • Mohsen Sheykhhasan (Department of Mesenchymal Stem Cells, The Academic Centre for Education, Culture and Research) ;
  • Leila Naserpoor (Department of Reproductive Biology, The Academic Centre for Education, Culture and Research) ;
  • Azar Sheikholeslami (Department of Mesenchymal Stem Cells, The Academic Centre for Education, Culture and Research)
  • 투고 : 2021.08.23
  • 심사 : 2021.09.30
  • 발행 : 2022.03.31
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초록

Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women's MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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과제정보

This work was supported by grants from the Academic Center for Education, Culture and Research, Qom Branch, Qom, Iran. In addition, we are grateful to all the women who participated in this study. We also thank the staff at the midwifery section of infertility treatment clinic of ACECR.

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