Parasites, Hosts and Diseases

  • 제60권5호
  • /
  • Pages.309-315
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  • 2022
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Treatment with Extracellular Vesicles from Giardia lamblia Alleviates Dextran Sulfate Sodium-Induced Colitis in C57BL/6 Mice

  • Kim, Hyun Jung (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency) ;
  • Lee, Young-Ju (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency) ;
  • Back, Seon-Ok (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency) ;
  • Cho, Shin-Hyeong (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency) ;
  • Lee, Hee-Il (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency) ;
  • Lee, Myoung-Ro (Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency)
  • 투고 : 2022.03.13
  • 심사 : 2022.08.08
  • 발행 : 2022.10.31
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초록

Inflammatory bowel disease (IBD) is a chronic and recurrent illness of the gastrointestinal tract. Treatment of IBD traditionally involves the use of aminosalicylic acid and steroids, while these drugs has been associated with untoward effects and refractoriness. The absence of effective treatment regimen against IBD has led to the exploration of new targets. Parasites are promising as an alternative therapy for IBD. Recent studies have highlighted the use of parasite-derived substances, such as excretory secretory products, extracellular vesicles (EVs), and exosomes, for the treatment of IBD. In this report, we examined whether EVs secreted by Giardia lamblia could prevent colitis in a mouse model. G. lamblia EVs (GlEVs) were prepared from in vitro cultures of Giardia trophozoites. Clinical signs, microscopic colon tissue inflammation, and cytokine expression levels were detected to assess the effect of GlEV treatment on dextran sulfate sodium (DSS)-induced experimental murine colitis. The administration of GlEVs prior to DSS challenge reduced the expression levels of pro-inflammatory cytokines, including tumor necrosis factor alpha, interleukin 1 beta, and interferon gamma. Our results indicate that GlEV can exert preventive effects and possess therapeutic properties against DSS-induced colitis.

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과제정보

This work was supported by a grant from the Korea Centers for Disease Control and Prevention (2017-NC54001-02).

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