Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of feeding raw potato starch on the composition dynamics of the piglet intestinal microbiome

  • Yi, Seung-Won (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Han Gyu (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • So, Kyoung-Min (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Eunju (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Young-Hun (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Minji (Animal Nutrition and Physiology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Jin Young (Animal Nutrition and Physiology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki Hyun (Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration) ;
  • Oem, Jae-Ku (Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University) ;
  • Hur, Tai-Young (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration) ;
  • Oh, Sang-Ik (Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration)
  • Received : 2022.01.28
  • Accepted : 2022.07.04
  • Published : 2022.11.01
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Abstract

Objective: Raw potato starch (RPS) is resistant to digestion, escapes absorption, and is metabolized by intestinal microflora in the large intestine and acts as their energy source. In this study, we compared the effect of different concentrations of RPS on the intestinal bacterial community of weaned piglets. Methods: Male weaned piglets (25-days-old, 7.03±0.49 kg) were either fed a corn/soybean-based control diet (CON, n = 6) or two treatment diets supplemented with 5% RPS (RPS5, n = 4) or 10% RPS (RPS10, n = 4) for 20 days and their fecal samples were collected. The day 0 and 20 samples were analyzed using a 16S rRNA gene sequencing technology, followed by total genomic DNA extraction, library construction, and high-throughput sequencing. After statistical analysis, five phyla and 45 genera accounting for over 0.5% of the reads in any of the three groups were further analyzed. Furthermore, short-chain fatty acids (SCFAs) in the day 20 fecal samples were analyzed using gas chromatography. Results: Significant changes were not observed in the bacterial composition at the phylum level even after 20 d post feeding (dpf); however, the abundance of Intestinimonas and Barnesiella decreased in both RPS treatment groups compared to the CON group. Consumption of 5% RPS increased the abundance of Roseburia (p<0.05) and decreased the abundance of Clostridium (p<0.01) and Mediterraneibacter (p< 0.05). In contrast, consumption of 10% RPS increased the abundance of Olsenella (p<0.05) and decreased the abundance of Campylobacter (p<0.05), Kineothrix (p<0.05), Paraprevotella (p<0.05), and Vallitalea (p<0.05). Additionally, acetate (p<0.01), butyrate (p<0.05), valerate (p = 0.01), and total SCFAs (p = 0.01) were upregulated in the RPS5 treatment group Conclusion: Feeding 5% RPS altered bacterial community composition and promoted gut health in weaned piglets. Thus, resistant starch as a feed additive may prevent diarrhea in piglets during weaning.

Keywords

Acknowledgement

This study was supported by the 2021 RDA Fellowship Program of the National Institute of Animal Science, Rural Development Administration, and was carried out with the support of the "Cooperative Research Program for Agriculture Science and Technology Development (Project title: Development of gut microbiota for preventing intestinal diseases and its impact on host immunity in pigs, Project No. PJ01564401)", Rural Development Administration, Republic of Korea.

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