Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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The Peripheral Immune Landscape in a Patient with Myocarditis after the Administration of BNT162b2 mRNA Vaccine

  • Yoon, Bo Kyung (Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine) ;
  • Oh, Tae Gyu (Gene Expression Laboratory, Salk Institute for Biological Studies) ;
  • Bu, Seonghyeon (Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Uijeongbu St. Mary's Hospital) ;
  • Seo, Kyung Jin (Department of Pathology, The Catholic University of Korea, Uijeongbu St. Mary's Hospital) ;
  • Kwon, Se Hwan (Department of Radiology, Kyung Hee University Medical Center) ;
  • Lee, Ji Yoon (KYNOGEN Co.) ;
  • Kim, Yeumin (KYNOGEN Co.) ;
  • Kim, Jae-woo (Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine) ;
  • Ahn, Hyo-Suk (Division of Cardiology, Department of Internal Medicine, The Catholic University of Korea, Uijeongbu St. Mary's Hospital) ;
  • Fang, Sungsoon (Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine)
  • Received : 2022.02.24
  • Accepted : 2022.06.09
  • Published : 2022.10.31
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Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed a serious threat to global public health. A novel vaccine made from messenger RNA (mRNA) has been developed and approved for use at an unprecedented pace. However, an increased risk of myocarditis has been reported after BNT162b2 mRNA vaccination due to unknown causes. In this study, we used single-cell RNA sequencing and single-cell T cell receptor sequencing analyses of peripheral blood mononuclear cells (PBMCs) to describe, for the first time, changes in the peripheral immune landscape of a patient who underwent myocarditis after BNT162b2 vaccination. The greatest changes were observed in the transcriptomic profile of monocytes in terms of the number of differentially expressed genes. When compared to the transcriptome of PBMCs from vaccinated individuals without complications, increased expression levels of IL7R were detected in multiple cell clusters. Overall, results from this study can help advance research into the pathogenesis of BNT162b2-induced myocarditis.

Keywords

Acknowledgement

This study was supported by Korea Health Technology R&D Project (HR18C0012) from the Ministry of Health & Welfare to S.F. and the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (NRF-2018R1A5A2025079). B.K.Y. was supported by the Seok-San Biomedical Science Scholarship, Yonsei University College of Medicine.

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