International Journal of Stem Cells

  • 제15권2호
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  • Pages.195-202
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  • 2022
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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Human Umbilical Cord Mesenchymal Stem Cells Improve the Necrosis and Osteocyte Apoptosis in Glucocorticoid-Induced Osteonecrosis of the Femoral Head Model through Reducing the Macrophage Polarization

  • Gang Tian (Department of Orthopedics, Weihai Central Hospital Affiliated to Qingdao University & Qingdao University) ;
  • Chuanjie Liu (Xinxiang Medical University) ;
  • Qi Gong (Weihai Key Laboratory of Autoimmunity & Central Laboratory of Weihai Central Hospital) ;
  • Zhiping Yu (Department of Sports Medicine, Weihai Central Hospital Affiliated to Qingdao University) ;
  • Haitao Wang (Department of Trauma Surgery, Weihai Central Hospital Affiliated to Qingdao University) ;
  • Daoqiang Zhang (Weihai Key Laboratory of Autoimmunity & Central Laboratory of Weihai Central Hospital) ;
  • Haibo Cong (Department of Orthopedics, Weihai Central Hospital Affiliated to Qingdao University & Weihai Key Laboratory of Autoimmunity)
  • 투고 : 2021.06.28
  • 심사 : 2021.10.25
  • 발행 : 2022.05.30
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초록

Background and Objectives: Apoptosis is an outstanding determinant of glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH). Human umbilical cord mesenchymal stem cells (hUC-MSCs) have been demonstrated to be associated with apoptosis in diseases models. However, the role of hUC-MSCs in GC-induced ONFH via regulating apoptosis still needs further study. Methods and Results: In the present study, a GC-induced ONFH model was built in vivo through a consecutive injection with lipopolysaccharide (LPS) and methylprednisolone. The necrosis and apoptosis of the femoral head was evaluated by histological and Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) assay. The level of collagen and TRAP positive cells were determined by Masson and TRAP staining, respectively. M1 macrophage polarization was assessed using immunofluorescence assay. The level of proinflammatory cytokines including tumor necrosis factor (TNF)-α, Interleukin (IL)-1β and IL-6 of femoral head was determined by enzyme-linked immunosorbent assay (ELISA) kits. The protein expression of AKT, mTOR, p-AKT and p-mTOR was detected using western blot assay. The results showed that hUC-MSCs treatment prominently promoted the GC-induced the decrease of the collagen level and the increase of TRAP positive cells. Besides, hUC-MSCs treatment decreased necrosis and apoptosis, macrophage polarization, the level of TNF-α, IL-1β and IL-6, the protein expression of p-AKT and p-mTOR, and the radio of p-AKT to AKT and p-mTOR to mTOR of femoral head in vivo. Conclusions: Therefore, the present study revealed that hUC-MSCs improved the necrosis and osteocyte apoptosis in GC-induced ONFH model through reducing the macrophage polarization, which was associated with the inhibition of AKT/mTOR signaling pathway.

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과제정보

This work was supported by Key R&D Projects of Shandong Province (2019GSF107007), and Special Fund for Taishan Scholars Project (TS201511110).

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