The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effect of Artemisiae Argyi Folium Extract on Alcohol-Induced Gastritis

애엽(艾葉) 추출물이 알코올성 위염에 미치는 효과

  • Lee, Jin A (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seo, Jeong Bok (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Jin Young (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center) ;
  • Roh, Seong-Soo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서정복 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김진영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (DHU 바이오융복합시험센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2022.09.14
  • Accepted : 2022.10.07
  • Published : 2022.09.30
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Abstract

Objective: Alcohol is known to cause inflammation in the stomach by decreasing the protective substances of the gastric mucosa and increasing oxidative stress. The purpose of this study was to investigate the anti-inflammatory effect of Artemisiae Argyi Folium extract (AF) on alcohol-induced gastritis. Methods: The total polyphenol and flavonoid contents of AF were confirmed through an in vitro experiment. Radical scavenging activities were confirmed using DPPH and ABTS assays. In vivo experiments were conducted on mice divided into 5 groups (n=8): a normal group (Nor), an alcohol-induced gastritis group (Con), an alcohol-induced gastritis group administered 10 mg/kg sucralfate (SC), an alcohol-induced gastritis group administered 100 mg/kg AF (AFL), and an alcohol-induced gastritis group administered 200 mg/kg AF(AFH). The serum levels of reactive oxygen species (ROS) were determined, and protein expressions were confirmed in gastric tissues. Results: In alcohol-induced gastritis, AF alleviated damage to the gastric mucosa caused by alcohol. AF also decreased the serum ROS levels. Western blots showed that AF decreased the expression of NADPH oxidase and decreased the expression of the NF-κB pathway associated with inflammation. AF also decreased the expression of adhesion molecules and chemokine proteins, and increased the expression of anti-inflammatory proteins. Conclusions: AF not only reduced oxidative stress in alcohol-induced gastritis, but it also relieved gastric mucosal inflammation by regulating the expression of the NF-κB pathway.

Keywords

Acknowledgement

이 연구는 2022년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다(No. 2018R1A5A2025272).

References

  1. Kim MJ, Chop HM, Nam WH, Kim SJ, Son SM, Kim JO. Anti-inflammatory and Anti-gastritis Effect of Sogunjung-tang Mixed Soft Extract. J Physiol & Pathol Koran Med 2021;35(6):235-41. https://doi.org/10.15188/kjopp.2021.12.35.6.235
  2. Kim OK, Nam DE, You YH, Jun WJ, Lee JM. Protective Effect of Canavalia gladiate on Gastric Inflammation Induced by Alcohol Treatment in Rats. J Korean Soc Food Sci Nutr 2013;42(5):690-6. https://doi.org/10.3746/jkfn.2013.42.5.690
  3. Liu ES, Cho CH. 2000. Relationship between ethanol-induced gastritis and gastric ulcer formation in rats. Digestion 2000;62(4):232-9. https://doi.org/10.1159/000007821
  4. Li G, Zhu L, Cao Z, Wang J, Zhou F, Wang X, et al. A New Participant in the Pathogenesis of Alcoholic Gastritis-: Pyroptosis. Cell Physiol Biochem 2018;49(1):406-18. https://doi.org/10.1159/000492902
  5. Nam DE, Kim OK, Shim TJ, Lee JK, Hwang KT. Inhibitory Effects of Chios Mastic Gum on Gastric Acid Secretion by Histamine-Related Pathway in a Rat Model and Primary Parietal Cells. J Korean Soc Food Sci Nutr 2014;43(10):1500-9. https://doi.org/10.3746/jkfn.2014.43.10.1500
  6. Lee SM, Kim MG, Lee SY, Kang TH. Effects of Artemisia primceps Extract on Bone Metabolism. J Korean Soc Food Sci Nutr 2010;39(3):363-8. https://doi.org/10.3746/jkfn.2010.39.3.363
  7. Gu L, Wang X, Shao X, Ding Y, Li Y. Study on chemical constituents of Folium Artemisiae argyi Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis. Saudi Pharm J 2022; 30(5):532-43. https://doi.org/10.1016/j.jsps.2022.02.018
  8. Park WS. Study on Biological Effect of Water Extract from ARTEMISIAE ARGI FOLIUMon Mouse Macrophage Raw 264.7 Cells. Korean J Oriental Physiology & Pathology 2008;22(4):815-20.
  9. Park WS. Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Viability of Human Hepatocyte Treated with Toxicants. Korean J Oriental Physiology &Pathology 2010;24(3):457-62.
  10. Rama P, Vignesh A, Lakshmanan G. In vitro antioxidant activity of achyranthes aspera linn. Int Med Pharm 2013;3(2):37-78.
  11. Jiao H, Wang SY. Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry. J Agric Food Chem 2000;48(11):5672-6. https://doi.org/10.1021/jf000765q
  12. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26(9-10):1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  14. Ali SF, LeBel CP, Bondy SC. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicology 1992;13(3):637-48.
  15. Hossen MJ, Chou JY, Li SM, Fu XQ, Yin C, Guo H, et al. An ethanol extract of the rhizome of Atractylodes chinensis exerts anti-gastritis activities and inhinits Akt/NF-kB signaling. J Ethnopharmacol 2019;228:18-25. https://doi.org/10.1016/j.jep.2018.09.015
  16. Siegmund SV, Singer MV. Effects of alcohol on the upper gastrointestinal tract and the pancreas--an up-to-date overview. Z Gastroenterol 2005;43(8):723-6. https://doi.org/10.1055/s-2005-858257
  17. Park JH, Kweon GR. Clinical Applications of Antioxidants. Hanyang Med Res 2013;33(2):130-6. https://doi.org/10.7599/hmr.2013.33.2.130
  18. Lee JA, Lee TJ, Kim JY, Shin MR, Park HJ, Roh SS. Anti-inflammatory effect of Uncariae Ramulus et Uncus on alcohol-induced gastritis. Kor J Herbol 2022;37(5):63-74.
  19. Kim MY, Kwon OJ, Noh JS, Roh SS. Inhibitory Activities of Water Extracts of Black Ginseng on HCl/Ethanol-Induced Acute Gastritis through Anti-Oxitant Effect. J Korean Soc Food Sci Nutr 2016;45(9):1249-56. https://doi.org/10.3746/jkfn.2016.45.9.1249
  20. Sergent O, Griffon B, Cillard P, Cillard J. Alcohol and oxidative stress. Pathol Biol (Paris) 2001;49(9):689-95. https://doi.org/10.1016/S0369-8114(01)00244-9
  21. Vermot A, Petit-Hartlein I, Smith SME, Fieschi F. NADPH Oxidases (NOX): An Overview from Discovery, Molecular Mechanisms to Physiology and Pathology. Antioxidants (Basel) 2021;10(6):890. https://doi.org/10.3390/antiox10060890
  22. Chae SW. Function and Activation of NF-kB in Immune System. Korean J Otolaryngol 2005; 48(3):284-8.
  23. Ahn SH, Kim JT, Shim HM. Wild Ginseng Exerts Anti-inflammatory Effects via NF-kB inactivation in RAW 264.7 Cells. Korean J Oriental Physiology & Pathology 2007;21(2):498-503.
  24. Na KS, Kang TY, Lee JS, Park YW, Lee HS, Uhm WS, et al. Interaction between Th1 Cytokine and Th2 Cytokine on the Expression of Chemokine and Adhesion Molecule in Human Mesangial Cells. J Rheum Dis 2004;11(2):77-89.
  25. Lee JH, Byun JA, Yeo SW, Kim JK, Park YS. Role of Interleukin-10 on Mouse Otitis Media Model Using Lipopolysaccharide. Korean J Otorhinolaryngol-Head Neck Surg 2011;54(1):43-7. https://doi.org/10.3342/kjorl-hns.2011.54.1.43