Journal of Plant Biotechnology

  • 제49권3호
  • /
  • Pages.240-249
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  • 2022
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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엽록소 형광반응을 이용한 인삼 유전자원의 습해 스트레스 평가

Evaluation of waterlogging tolerance using chlorophyll fluorescence reaction in the seedlings of Korean ginseng (Panax ginseng C. A. Meyer) accessions

  • 지무근 (충청남도농업기술원 인삼약초연구소) ;
  • 홍영기 (충청남도농업기술원 인삼약초연구소) ;
  • 김선익 (충청남도농업기술원 인삼약초연구소) ;
  • 박용찬 (충청남도농업기술원 인삼약초연구소) ;
  • 이가순 (충청남도농업기술원 인삼약초연구소) ;
  • 장원석 (충청남도농업기술원 인삼약초연구소) ;
  • 권아름 (충청남도농업기술원 인삼약초연구소) ;
  • 성봉재 (충청남도농업기술원 인삼약초연구소) ;
  • 김미선 (충북대학교 식물자원학과) ;
  • 조용구 (충북대학교 식물자원학과)
  • Jee, Moo Geun (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Hong, Young Ki (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Kim, Sun Ick (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Park, Yong Chan (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Lee, Ka Soon (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Jang, Won Suk (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Kwon, A Reum (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Seong, Bong Jae (Ginseng & Medicinal Plant Research Institute, CNARES) ;
  • Kim, Me-Sun (Department of Crop Science, Chungbuk National University) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
  • 투고 : 2022.09.08
  • 심사 : 2022.09.13
  • 발행 : 2022.09.30
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초록

본 연구는 인삼 습해 피해 설정을 확립하고 유전자원을 대상으로 엽록소형광 기법을 이용하여 습해 스트레스 수준을 평가하기 위해 수행되었다. 습해 스트레스 평가는 2년생 묘삼 57개 유전자원 대상으로 25일간 지제부까지 담수처리를 하였다. 피해 정도에 따라 5개 등급으로 분류하여 저항성 유전자원 3개, 감수성 유전자원 3개를 선발하였다. 인삼 유전자원 57개의 지하부 생체중 및 엽록소 함량은 대부분 감소하였고 저항성 유전자원보다 감수성 유전자원의 감소율이 크게 나타났다. 5개의 엽록소형광 매개변수는 습해 스트레스에 노출되는 기간이 길어질수록 증감하는 경향을 보였으며, 특히, Fv/Fm값이 0.8보다 낮으면 광계II의 반응중심이 손상을 입은 상태로 감수성 유전자원의 경우 습해 스트레스에 노출될수록 감소하였다. 본 연구결과에서 유전자원 57개의 최대양자수율(Fv/Fm)은 습해 스트레스 하에서 차이를 보였으며, 습해 스트레스에 저항성을 가진 습해 저항성 유전자원과 감수성 유전자원 간의 차이를 확인할 수 있는 매개변수로 판단되어 인삼 유전자원에 대해 습해 스트레스 수준을 평가할 수 있는 도구로 활용 가능하다고 사료된다.

Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

키워드

과제정보

본 연구는 농촌진흥청에서 주관하는 지역특화작목기술개발(과제번호 PJ016151)의 연구비 지원에 의해 이루어진 결과로 이에 감사드립니다.

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