Journal of Practical Engineering Education (실천공학교육논문지)

Korean Institute for Pratical Engineering Education (한국실천공학교육학회)
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Educational Peptide Mapping of Protein-based Biopharmaceuticals by using LC-MS/MS

LC-MS/MS를 이용한 단백질 의약품 맵핑 교수법

  • Kim, Junseok (Department of Biomedical Systems Engineering, Korea Polytechnics)
  • 김준석 (한국폴리텍대학 분당융합기술교육원 생명의료시스템과)
  • Received : 2022.06.21
  • Accepted : 2022.08.22
  • Published : 2022.08.31
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Abstract

This experiment presents a precise analysis method using a mass spectrometer in the biopharmaceutical market, where utility is expanding. Among various techniques for analyzing the protein drug, somatotropin, the peptide fragments through biochemical sample preparation was analyzed by LC-MS/MS characterization. The analysis process was performed by separation analysis using nanoUPLC and MS/MS analysis using Orbitrap. In the case of somatotropin with 21 tryptic peptides, 13 of them were consistent with theoretical predictions within an average of 1 ppm error.

본 실험은 효용성이 넓어지고 있는 바이오의약품 시장에서 질량분석기를 이용한 정밀 분석법을 제시한다. 단백질 의약품인 인성장호르몬(Somatotopin)을 분석하는 다양한 기술 중, 생화학적 시료 전처리를 통해 펩티드화 시킨 단백질을 LC-MS/MS 분석법으로 분석하였다. 분석과정은 액체크로마토그래피를 이용한 분리분석과 질량분석을 이용한 MS 및 MS/MS 분석으로 수행하였다. 인성장호르몬의 경우 21개의 트립틱 펩티드로 절단할 수 있는데, 본 실험을 통해 이들 중 13개의 펩티드가 평균 1 ppm 에러 이내로 이론적 예측치와 일치하였다.

Keywords

References

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