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Lactiplantibacillus plantarum SM4 를 이용한 레드비트 생물전환물의 항산화, 피부 미백과 자외선 흡수 효능 연구

A Study on Antioxidant, Skin-Whitening and UV Absorption Effect of Beta vulagaris Using Lactiplantibacillus plantarum SM4

  • 염서희 (선문대학교 식품과학부) ;
  • 강민호 (선문대학교 식품과학부) ;
  • 박제훈 (선문대학교 식품과학부) ;
  • 김소희 (선문대학교 식품과학부) ;
  • 김진우 (선문대학교 식품과학부)
  • Yeom, Suh Hee (Department of Food Science, Sun Moon University) ;
  • Kang, Min Ho (Department of Food Science, Sun Moon University) ;
  • Park, Jae Hoon (Department of Food Science, Sun Moon University) ;
  • Kim, So Hee (Department of Food Science, Sun Moon University) ;
  • Kim, Jin Woo (Department of Food Science, Sun Moon University)
  • 투고 : 2022.08.11
  • 심사 : 2022.09.20
  • 발행 : 2022.09.30

초록

본 연구의 목적은 Lactiplantibacillus plantarum SM4를 이용하여 Beta vulagaris의 생물전환물(BBE)의 피부미백 및 자외선 흡수 효과를 확인하기 위한 것으로 총 폴리페놀 함량(TPC), 총 플라보노이드 함량(TFC), 라디칼 소거 활성(RSA), 티로시나아제 활성 억제(TAI)를 측정하여 항산화 활성 및 미백활성을 평가하였다. BBE의 TPC와 TFC는 25.0 mg GAE/g DM, 8.05 mg QE/g DM으로 각각 열수 추출물(HWE) 대비 1.3, 1.1 배 높았다. 항산화 활성 지표인 RSA와 피부 미백 효과 지표인 TAI가 각각 36.8%, 68.6%로 HWE보다 1.1, 1.2 배 높았다. BBE의 UVA와 UVB 흡수율은 각각 21.4, 87.6%로 HWE보다 1.4, 1.7 배 높았다. 또한 LC-MS/MS를 이용한 BBE의 주요 물질을 분석을 통해 항산화 및 피부 미백 효과가 높은 것으로 알려진 폴리페놀의 일종인 kaempferol과 isorhamnetin이 확인됐다. 이에 따라 BBE는 항산화, 피부 미백, 자외선 흡수 특성이 우수해 기능성 화장품 소재로 활용될 것으로 기대된다.

The purpose of this study was to confirm the skin whitening and UV absorption effects of bioconverted Beta vulagaris (BBE) using Lactiplantibacillus plantarum SM4, and the effects were evaluated by measuring total polyphenol content (TPC), total flavonoid content (TFC), radical elimination activity (RSA), and tyrosinase activity inhibition (TAI). TPC and TFC of BBE were 25.0 mg GAE/g DM and 8.05 mg QE/g DM, which were 1.3 and 1.1 times higher than hot-water extract (HWE) respectively. RSA, an indicator of antioxidant activity, and TAI, an indicator of skin-whitening effect, were 36.8%, and 68.6%, respectively, 1.1 and 1.2 times higher than that of HWE. UVA and UVB absorption of BBE were 21.4 and 87.6%, which was 1.4 and 1.7 times higher than that of HWE respectively. When main substances of BBE were analyzed using LC-MS/MS, kaempferol and isorhamnetin, a type of polyphenols known to have high antioxidant and skin-whitening effects, were identified. As a results, BBE is expected to be used as a functional cosmetic material as it has excellent antioxidant, skin whitening, and UV absorption properties.

키워드

참고문헌

  1. J. W. Se o, M. S. Ryu, H. J. Yang, S. J. Je ong, and D. Y. Jeong, The antioxidant and skin-whitening effects of Saccharomyces cerevisiae FT4-4 isolated from berries grown in Sunchang, J. Life Sci., 31(2), 175 (2021). https://doi.org/10.5352/JLS.2021.31.2.175
  2. J. E. Park, H. J Kim, S. N. Kim, S. H. Kang, and Y. J. Kim, Inhibitory e ffe ct of Rosa multiflora hip e xtract on UVB-induced skin photoaging in Hs68 fibroblasts, J. Soc. Cosmet. Sci. Korea, 41(4), 351 (2015). https://doi.org/10.15230/SCSK.2015.41.4.351
  3. J. H. Jang, C. Lee, S. C. Kim, J. W. Chung, and C. I. Park, Protective effect of marine natural products against UVB-induced damages in human skin fibroblast via antioxidant mechanism, J. Soc. Cosmet. Sci. Korea, 36(1), 79 (2010).
  4. Y. S. Lee, Effects of reactive oxygen species induced by arachidonic Acid on the stimulation of melanin synthesis in B16 me lanoma ce lls, Arch. Pharm. Res., 61(5), 248 (2017).
  5. Y. J. Le e and C. G. Hyun, Me chanistic insights into the ameliorating effect of melanogenesis of psoralen derivatives in B16F10 melanoma cells, Molecules., 27(9), 2613 (2022). https://doi.org/10.3390/molecules27092613
  6. E. K. Kim, H. B. Lee, H. B. Lee, and C. Y. Lee, Trend of depigmenting research based on patent analysis. J. Soc. Cosmet. Sci. Korea, 33(4), 209 (2007)
  7. K. S. Lee, M. G. Kim, and K. Y. Lee, Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf, J. Korean Soc. Food Sci. Nutr., 35(2), 182 (2006) https://doi.org/10.3746/JKFN.2006.35.2.182
  8. H. K. Bae and S. H. You, Biological activity study on anti-oxidant, whitening, and anti-inflammatory effects of Astragalus membranaceus ethanol extracts and bioconversion extracts, Asian J. Beauty Cosmetol., 15(4), 489 (2017) https://doi.org/10.20402/ajbc.2017.0167
  9. C. H. Park, Y. R. Choi, K. J. Yum, and M. J. Kim, A novel strain of Saccharomyces servazzii, ceb-kc-011 from young radish kimchi:optimization of the growth condition and inhibition of α-glucosidase by bioconverted Rhynchosia nulubilis using ceb-kc-011, J. Korean Soc. Food Sci. Nutr., 49(3), 236 (2020). https://doi.org/10.3746/jkfn.2020.49.3.236
  10. K. J. Lee, B. H. Lee, J. P. Mun, C. Lian, and J. Y. Ma, Analysis of bioconversion components of fermentation hwangryunhaedok-tang, Arch. Pharm. Res., 57(4), 293 (2013)
  11. J. Y. Kim and H. K. Kim, Physiological activity of redbeet, Bulletin of Food Tech., 22(3), 537 (2009).
  12. S. Y. Park, J. H. LEE, B. M. Kim, S. J. Baek, S. H. Lee, and J. S. Nam, Comparison of nutritional components of beets from three different Beta vulgaris L. cultivars in Korea, J. Korean. Soc. Food Sci. Nutr., 49(9), 969 (2020). https://doi.org/10.3746/jkfn.2020.49.9.969
  13. J. W. Hong, H. Y. Park, J. H. Kim, S. H. Yeom, and J. W. Kim, Antioxidation and anti-photoaging effects of white Taraxacum Coreanum Extract by Lactobacillus plantarum, JKAIS, 22(4), 554 (2021).
  14. D. H. Gam, J. M. Jo, H. J. Jung, and J. W. Kim, Optimization of extraction conditions of antioxidant activity and bioactive compounds from rice bran by response surface methodology, Appl. Chem. Eng., 29(6), 726 (2018). https://doi.org/10.14478/ACE.2018.1064
  15. H. F. Al-Sayye d, R. A. Al-Kurd, I. F. Mahmoud, S. M. Abde lQade r, D. H. Swe idan, L. T. Rize q, and M. M. Mwalla, Developing a database for total phenolic content, total flavonoid content, and antioxidant activity of Jordanian crops, Int. J. Food Prop., 25(1), 1290 (2022). https://doi.org/10.1080/10942912.2022.2077369
  16. S. J. Kim, D. Kim, J. Park, and T. K. Le e , Phe nolic content, DPPH radical scavenging, and tyrosinase inhibitory activities of Ecklonia cava extracted with the ultrasonic wave method, J. Life Sci., 23(7), 913 (2013). https://doi.org/10.5352/JLS.2013.23.7.913
  17. J. S. Le e , J. A. Kim, S. H. Cho, A. R. Son, T. S. Jang, M. S. So, S. R. Chung, and S. H. Lee, Tyrosinase inhibitors isolated from the roots of Glycyrrhiza glabra L., Kor. J. Pharmacogn., 34(1), 33 (2003).
  18. M. H. Yu, H. G Im, H. J. Lee, Y. J. Ji, and I. S. Lee, Components and their antioxidative activities of methanol extracts from sarcocarp and seed of Zizyphus jujuba var. inermis Rehder, Korean J. Food Sci Tech., 38(1), 128 (2006).
  19. M. J. In, K. H. Kim, and D. C. Kim, Antioxidant and anticoagulant activities of Ganghwa medicinal mugwort (Artemisia princeps Pampanini) extract, J. Appl. Biol. Chem., 63(4), 439 (2020). https://doi.org/10.3839/jabc.2020.057
  20. S. Y. Park, S. H. Lee, and J. S. Nam, Comparison of the Antioxidant properties and phenolic compositions of diffe re nt varie tie s of be e ts (Beta vulgaris L.) cultivated in Korea, J. Korean. Soc. Food Sci. Nutr., 50(10), 1058 (2021). https://doi.org/10.3746/jkfn.2021.50.10.1058
  21. B. N. Song, D. B. Lee, S. H. Lee, B. R. Park, J. h. Choi, Y. S. Kim, and S. Y. Park, Physicoche mical properties and antioxidant activity of extract from Astragalus membranaceus Bunge leaf fermented with lactic acid bacteria, KJMCS, 28(6), 428 (2020). https://doi.org/10.7783/KJMCS.2020.28.6.428
  22. M. J. Kim, N. R. Shin, M. J. Lee, and H. Kim, Microorganisms involved in natural fermentation of Asparagus cochinchinensis roots and change s in e fficacie s after fermentation, J. Korean. Med. Obes. Res., 18(2), 96 (2018). https://doi.org/10.15429/jkomor.2018.18.2.96
  23. H. S. Song, S. H. Eom, Y. M. Kang, J.D. Choi, and Y. M. Kim, Enhancement of the antioxidant and anti-Inflammatory activity of Hizikia fusiforme water extract by lactic acid bacteria fermentation, K. J. Fish Aquat. Sci., 44(2), 111 (2011). https://doi.org/10.5657/KFAS.2011.44.2.111
  24. P. Vijayabaskar and V. Shiyamala, Antioxidant properties of seaweed polyphenol from Turbinaria ornata (Turne r), J. Agardh, Asian P ac. J. Trop. Biomed., 2(1), 90 (2012).
  25. T. W. Jang, C. G. Oh, and J. H. Park, Antioxidant activity and protective effects on oxidative DNA damage of Smilax china root, J. Appl. Biol. Chem., 61(2), 109 (2018). https://doi.org/10.3839/jabc.2018.016
  26. S. Chawla, M. A. DeLong, M. O Visscher, R. R. Wicke tt, P. Manga and R. E. Boissy, Me chanism of tyrosinase inhibition by deoxyarbutin and its second- generation derivatives, Br. J. Dermatol., 159(6), 1267 (2008). https://doi.org/10.1111/j.1365-2133.2008.08864.x
  27. J. H. Lee, Y. C. Yoon, J. K. Kim, Y. E. Park, H. S. Hwang, G. S. Kwon, and J. B. Lee, Antioxidant and whitening effects of the fermentation of barley seeds (Hordeum vulgare L.) using lactic acid bacteria, J. Life Sci., 28(4), 444 (2018). https://doi.org/10.5352/JLS.2018.28.4.444
  28. J. Solimine, E. Garo, J. Wedler, K. Rusanov, O. Fertig, M. Hamburge r, and V. Butte rwe ck. Tyrosinase inhibitory constituents from a polyphenol enriched fraction of rose oil distillation wastewater, Fitoterapia, 108, 13 (2016). https://doi.org/10.1016/j.fitote.2015.11.012
  29. H. Lin, K. C. Che ng, J. A. Lin, L. P. Hsie h, C. H. Chou, Y. Y. Wang and C. W. Hsieh, Pholiota nameko Polysaccharides protect against ultraviolet A-induced photoaging by regulating matrix metalloproteinases in human dermal fibroblasts, Antioxidants, 11(4), 739 (2022). https://doi.org/10.3390/antiox11040739
  30. Y. Yang, R. Wu, D, Sargsyan, R. Yin, H. C. Kuo, I. Yang, L. Wang, D. Cheng, C. Wang, S. Li, R. Hudlikar. Y. Lu, and A. Kong, UVB drive s diffe re nt stage s of epigenome alterations during progression of skin cancer, Cancer Lett., 449, 20 (2019). https://doi.org/10.1016/j.canlet.2019.02.010
  31. K. Y. Nam and J. S. Lee, Characteristics and dyeability of Juniperus chinensis extracts, Korea J. Hum. Ecol., 21(5), 989 (2012). https://doi.org/10.5934/KJHE.2012.21.5.989
  32. F. Morales Iribas, Z. G. Cerovic, I. Moya. Time-resolved blue-green fluorescence of sugar beet (Beta vulgaris L.) leaves. Spectroscopic evidence for the presence of ferulic acid as the main fluorophore of the epidermis, Biochim. Biophys. Acta. Bioenerg., 1273, 251 (1996). https://doi.org/10.1016/0005-2728(95)00153-0
  33. K. A. Corleto, J. Singh, G. K. Jayaprakasha, and B. S. Patil, Storage stability of dietary nitrate and phenolic compounds in beetroot (Beta vulgaris) and arugula (Eruca sativa) juices, J. Food Sci., 83(5), 1237 (2018). https://doi.org/10.1111/1750-3841.14129
  34. X. Y. Xue, T. Miyakawa, Y. Hayashi, K. Okamoto, F. Hu, N. Mitani, and M. Tanokura, Isolation and tyrosinase inhibitory effects of polyphenols from the leaves of Persimmon, Diospyros kaki, J. Agric. Food Chem., 59(11), 6011 (2011). https://doi.org/10.1021/jf200940h
  35. J. H. Kim, H. Y. Kim, and E. J. Cho, Protective effects of kaempferol, quercetin, and its glycosides on amyloid beta-induced neurotoxicity in C6 glial cell. J. Korean Soc. Appl. Biol. Chem., 62(4), 327 (2019). https://doi.org/10.3839/jabc.2019.045
  36. G. Dhingra, P. Dhakad, and S. Tanwar, Review on phytochemical constituents and pharmacological activities of plant Calendula officinalis Linn. Proc. Biol. Sci,. 2(2), 216 (2022).