The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Survey of Oxolinic Acid-Resistant Erwinia amylovora in Korean Apple and Pear Orchards, and the Fitness Impact of Constructed Mutants

  • Ham, Hyeonheui (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Oh, Ga-Ram (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Park, Dong Suk (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Yong Hoon (Division of Biotechnology, Jeonbuk National University)
  • 투고 : 2022.04.26
  • 심사 : 2022.08.09
  • 발행 : 2022.10.01
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초록

Fire blight caused by Erwinia amylovora (Ea) is a devastating disease in apple and pear trees. Oxolinic acid (OA), a quinolone family antibiotic that inhibits DNA gyrase, has been employed to control fire blight in South Korea since 2015. The continuous use of this bactericide has resulted in the emergence of OA-resistant strains in bacterial pathogens in other countries. To investigate the occurrence of OA-resistant Ea strains in South Korea, we collected a total of 516 Ea isolates from diseased apple and pear trees in 2020-2021 and assessed their sensitivities to OA. We found that all isolates were susceptible to OA. To explore the possibility of emerging OA-resistant Ea by continuous application of OA, we exposed Ea stains to a range of OA concentrations and constructed OA-resistant mutant strains. Resistance was associated with mutations in the GyrA at codons 81 and 83, which result in glycine to cysteine and serine to arginine amino acid substitutions, respectively. The in vitro growth of the mutants in nutrient media and their virulence in immature apple fruits were lower than those of wild-type. Our results suggest that OA-resistance decreases the fitness of Ea. Future work should clarify the mechanisms by which OA-resistance decreases virulence of this plant pathogen. Continuous monitoring of OA-resistance in Ea is required to maintain the efficacy of this potent bactericide.

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과제정보

This study was carried out with the support of Cooperative Research Programs (Project No. PJ01505902) from Rural Development Administration, Republic of Korea.

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