The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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3-Acetyl-11-Keto-Beta-Boswellic Acid from Boswellia serrata Attenuates Monosodium Iodoacetate-induced Osteoarthritis by Chondroprotective and Anti-inflammatory Effects

Monosodium iodoacetate로 유발된 골관절염 쥐에 유향(乳香) 성분 3-Acetyl-11-Keto-Beta-Boswellic Acid의 연골보호 및 항염증 효과

  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Hak Joo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center) ;
  • Choi, Hwang-Yong (Ju Yeong NS Co. Ltd.) ;
  • Kim, Hwa-Young (Ju Yeong NS Co. Ltd.) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 김민주 (대구한의대학교 한의과대학 본초학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초학교실) ;
  • 최학주 (대구한의대학교 한의과대학 본초학교실) ;
  • 박해진 (DHU 바이오융복합시험센터) ;
  • 최황용 (주영엔에스(주)) ;
  • 김화영 (주영엔에스(주)) ;
  • 노성수 (대구한의대학교 한의과대학 본초학교실)
  • Received : 2022.08.01
  • Accepted : 2022.09.25
  • Published : 2022.09.30
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Abstract

Objectives : 3-Acetyl-11-keto-𝛽-boswellic acid (AKBA) is a major active compound in Boswellia serrata. We investigated the arthritic changes following AKBA administration in monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : All rats were randomly divided into five groups: Normal, Control, INDO (indomethacin 2 mg/kg treated), AKBA30 (AKBA 30 mg/kg treated), and AKBA60 (AKBA 60 mg/kg treated); drugs were given 2 weeks before MIA injection. For all groups except the normal group, 50 µL of sterile saline with MIA (80 mg/mL) was injected into the right knee joint 2 weeks after drug administration. The drug administration was continued for 4 weeks from 1 week after osteoarthritis induction. The histomorphological changes of knee joint cartilage were observed by H&E staining. Also, the levels of glycosaminoglycan (GAG), cartilage oligomeric matrix protein (COMP), 5-lipoxygenase (5-LOX), 5-LOX-activating protein (FLAP), and leukotriene B4 (LTB4) in the knee joint were determined by the ELISA kits. The expressions of mitogen-activated protein kinases (MAPKs), inflammatory cytokines, and matrix metalloproteinases (MMPs) in knee joint were detected by Western blot. Results : Data show that levels of 5-LOX, FLAP, LTB4, and COMP were downregulated significantly in the AKBA treated groups when compared to those in the Control group. On the other hand, GAG levels were significantly elevated. As a result of Western blot, the AKBA-treated groups significantly inhibited phosphorylation of MAPKs. In addition, significant downregulation of the expression of inflammatory cytokines and MMPs was found in the AKBA-treated groups. Conclusion : Our findings suggest that administration of AKBA could exert better chondroprotective and anti-inflammatory effects for MIA-induced osteoarthritis rats.

Keywords

Acknowledgement

본 성과물은 2022년도 정부(과학기술정보통신부)의 재원으로 한국연구재단(No. 2018R1A5A2025272)과 주영엔에스(주)의 지원에 의해 수행되었습니다.

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