Ocean and Polar Research

  • 제44권3호
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  • Pages.209-220
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  • 2022
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  • 1598-141X(pISSN)
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  • 2234-7313(eISSN)
한국해양과학기술원 (Korea Institute of Ocean Science & Technology)
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해양 와편모조류 Prorocentrum minimum 기원 신규 탄산무수화효소(CAs) 유전자 3종의 차등 pH 대응 발현

Differential Expression of Three Novel Carbonic Anhydrases (CAs) Genes in Marine Dinoflagellate Prorocentrum minimum Against Various pH Conditions

  • 신정민 (상명대학교 생명화학공학부 생명공학과) ;
  • 이하은 (상명대학교 생명화학공학부 생명공학과) ;
  • 김한솔 (상명대학교 생명화학공학부 생명공학과) ;
  • 기장서 (상명대학교 생명화학공학부 생명공학과)
  • Shin, Jeongmin (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University) ;
  • Lee, Ha-Eun (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University) ;
  • Kim, Han-Sol (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University) ;
  • Ki, Jang-Seu (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University)
  • 투고 : 2021.11.16
  • 심사 : 2021.12.07
  • 발행 : 2022.09.30
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초록

Carbonic anhydrase (CA) is a key controller of the carbon concentrating mechanism (CCM), and is known to be affected by ambient pH and CO2 compositions. Herein, we characterized three novel CAs genes (PmCA1, 2, and 3) from the marine dinoflagellate Prorocentrum minimum, and evaluated the relative expressions of the PmCAs and photosynthetic genes PmatpB and PmrbcL under different pH conditions. Each PmCA was predicted to have amino acid residues constituting the zinc binding site. With signal peptide, PmCA1 and PmCA2 were predicted to be intracellular CAs located in the cytoplasm and chloroplast membrane, respectively. On the other hand, PmCA3 was predicted to be extracellular CA located in the plasma membrane. Also, PmCA1 was classified into the beta family, and PmCA2 and PmCA3 were classified into the alpha family via phylogenic analysis. The photosynthesis efficiency of P. minimum was similar at pH 7 to 9, and decreased significantly at pH 6 and pH 10. Overall, relative gene expression levels of the three PmCAs decreased at low pH, and increased as pH increased. Photosynthesis related genes, PmatpB and PmrbcL, showed similar expression patterns to those of PmCAs. These results suggest that changes in seawater pH may affect photosynthesis and CO2 metabolism in marine dinoflagellates.

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과제정보

이 성과는 2021년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구입니다(No. 2020R1A2C2013373).

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