Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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HPLC-UVD method validation for quantitative analysis of camelliaside A in hot-water extract of soybean (Glycine max L.) leaves

콩잎 열수추출물의 지표성분인 camelliaside A의 정량분석을 위한 HPLC-UVD 분석법 밸리데이션

  • Kim, Jeong Ho (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University) ;
  • Lee, Seung Hwan (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University) ;
  • Moon, Si Won (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University) ;
  • Park, Ki Hun (Division of Applied Life Science (BK21 plus), IALS, Gyeongsang National University)
  • Received : 2022.07.28
  • Accepted : 2022.08.30
  • Published : 2022.09.30
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Abstract

Soybean (Glycine max L.) leaves have been researched as functional food stuff actively, but there is no validation method to control quality of soybean leaves (SL). In this study, we annotated seven kaempferol derivatives to confirm camelliaside A as index metabolite in SL using UHPLC-ESI-TOF-MS. HPLC-UVD validation method of camelliaside A in hot-water extract of SL was established according to validation guideline of functional foods from the Ministry of Food and Safety of Korea. The HPLC-UVD method was validated with reliable parameters for examining specificity, accuracy, precision, limit of detection and quantification and linearity. The established method gave the suitable ranges to qunatitate camelliaside A from the hot-water extract of soybean leaves.

콩잎의 기능성식품소재연구가 활발히 진행되고 있지만 기능성 식품소재로서 콩잎을 관리할 수 있는 밸리데이션 방법은 제시되지 않고있다. 본 연구에서는 콩잎에서 7개의 kaempferol 유도체를 UHPLC-ESI-Q-TOF-MS로 동정하고 이들 중 camelliaside A를 지표물질로 선정하였다. 콩잎열수추출물에서 camelliaside A를 정량할 수 있는 HPLC-UVD 밸리데이션 방법을 식품의약품안전처의 건강기능성식품에 대한 가이드라인에 따라 구축하였다. 개발된 HPLC-UVD 밸리데이션 방법은 특이성, 정확도, 일내정밀도, 일간정밀도, 검출한계, 정량한계 및 직선성에서 가이드라인을 만족하는 결과값으로 검증되었다. 또한 camelliaside A 분석법을 콩잎열수추출물에 적용하여 적합한 수준의 평가값을 얻었다.

Keywords

Acknowledgement

This research was supported by the Bio and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science and ICT (No. NRF2020M3A9I3038523) and Rural Development Administration (PJ015732), Republic of Korea. The BK21 plus program supported scholarships for all students. The researchers received no external funding

References

  1. Kim IS (2022) Current perspectives on the beneficial effects of soybean isoflavones and their metabolites on plants. Food Sci Biotechnol 31: 515-526. doi: 10.1007/s10068-022-01070-7
  2. Sugiyama A (2019) The soybean rhizosphere: Metabolites, microbes, and beyond-A review. J Adv Res 19: 67-73. doi: 10.1016/j.jare.2019.03.005
  3. Ban YJ, Song YH, Kim JY, Baiseitova A, Lee KW, Kim KD, Park KH (2020) Comparative investigation on metabolites changes in soybean leaves by ethylene and activation of collagen synthesis. Ind Crops Prod 154: 112743. doi: 10.1016/j.indcrop.2020.112743
  4. Yuk HJ, Lee JH, Curtis-Long MJ, Lee JW, Kim YS, Ryu HW, Park CG, Jeong TS, Park KH (2011) The most abundant polyphenol of soy leaves, coumestrol, displays potent α-glucosidase inhibitory activity. Food Chem 126: 1057-1063. doi: 10.1016/j.foodchem.2010.11.125
  5. Yuk HJ, Curtis-Long MJ, Ryu HW, Jang KC, Seo WD, Kim JY, Kang KY, Park KH (2011) Pterocarpan profiles for soybean leaves at different growth stages and investigation of their glycosidase inhibitions. J Agric Food Chem 59: 12683-12690. doi: 10.1021/jf203326c
  6. Kim UH, Yoon JH, Li H, Kang JH, Ji HS, Park KH, Shin DH, Park HY, Jeong TS (2014) Pterocarpan-enriched soy leaf extract ameliorates insulin sensitivity and pancreatic β-cell proliferation in type 2 diabetic mice. Molecules 19: 18493-18510. doi: 10.3390/molecules 191118493
  7. Ryu R, Jeong TS, Kim YJ, Choi JY, Cho SJ, Kwon EY, Jung UJ, Ji HS, Shin DH, Choi MS (2016) Beneficial effects of pterocarpan-high soybean leaf extract on metabolic syndrome in overweight and obese Korean subjects: Randomized controlled trial. Nutrients 8. doi: 10.3390/nu8110734
  8. Li H, Ji HS, Kang JH, Shin DH, Park HY, Choi MS, Lee CH, Lee IK, Yun BS, Jeong TS (2015) Soy Leaf Extract Containing Kaempferol Glycosides and Pheophorbides Improves Glucose Homeostasis by Enhancing Pancreatic β-Cell Function and Suppressing Hepatic Lipid Accumulation in db/db Mice. J Agric Food Chem 63: 7198-7210. doi:10.1021/acs.jafc.5b01639
  9. Lee YH, Yuk HJ, Park KH, Bae YS (2013) Coumestrol induces senescence through protein kinase CKII inhibition-mediated reactive oxygen species production in human breast cancer and colon cancer cells. Food Chem 141: 381-388. doi: 10.1016/j.foodchem. 2013.03.053
  10. Li H, Kim UH, Yoon JH, Ji HS, Park HM, Park HY, Jeong TS (2019) Suppression of Hyperglycemia and Hepatic Steatosis by Black-Soybean-Leaf Extract via Enhanced Adiponectin-Receptor Signaling and AMPK Activation. J Agric Food Chem 67: 90-101. doi: 10.1021/acs.jafc.8b04527
  11. Yuk HJ, Song YH, Curtis-Long MJ, Kim DW, Woo SG, Lee YB, Uddin Z, Kim CY, Park KH (2016) Ethylene Induced a High Accumulation of Dietary Isoflavones and Expression of Isoflavonoid Biosynthetic Genes in Soybean (Glycine max) Leaves. J Agric Food Chem 64: 7315-7324. doi: 10.1021/acs.jafc.6b02543
  12. Xie C liang, Kang SS, Cho KM, Park KH, Lee DH (2018) Isoflavone-enriched soybean (Glycine max) leaves prevents ovariectomy-induced obesity by enhancing fatty acid oxidation. J Funct Foods 43: 165-172. doi: 10.1016/j.jff.2018.02.014
  13. Xie CL, Park KH, Kang SS, Cho KM, Lee DH (2021) Isoflavone-enriched soybean leaves attenuate ovariectomy-induced osteoporosis in rats by anti-inflammatory activity. J Sci Food Agric 101: 1499-1506. doi: 10.1002/jsfa.10763
  14. Korea Food & Drug Administration (2021) Guideline for Recognition of Functional Ingredients for Health Functional Foods, Cheongju