ALGAE

  • 제37권3호
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  • Pages.239-247
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  • 2022
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  • 1226-2617(pISSN)
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  • 2093-0860(eISSN)
한국조류학회(藻類) (The Korean Society of Phycology)
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Structural characterization and anti-inflammatory activity of fucoidan isolated from Ecklonia maxima stipe

  • Lee, Hyo-Geun (Department of Marine Life Sciences, Jeju National University) ;
  • Nagahawatta, D.P. (Department of Marine Life Sciences, Jeju National University) ;
  • Liyanage, N.M. (Department of Marine Life Sciences, Jeju National University) ;
  • Jayawardhana, H.H.A.C.K. (Department of Marine Life Sciences, Jeju National University) ;
  • Yang, Fengqi (Department of Marine Life Sciences, Jeju National University) ;
  • Je, Jun-Geon (Department of Marine Life Sciences, Jeju National University) ;
  • Kang, Min-Cheol (Research Group of Food Processing, Korea Food Research Institute) ;
  • Kim Hyun-Soo (National Marine Biodiversity Institute of Korea) ;
  • Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University)
  • 투고 : 2022.08.03
  • 심사 : 2022.09.12
  • 발행 : 2022.09.15
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초록

Enzyme-assisted hydrolysis is frequently used as a cost-effective and efficient method to obtain functional ingredients from bioresources. This study involved the enzyme-assisted hydrolyzation and purification of fucoidan from Ecklonia maxima stipe and the investigation of its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Fucoidans of Viscozyme-assisted hydrolysate from E. maxima (EMSFs) harvested in Jeju, Korea. Structural and chemical characterizations were performed using fourier transform infrared spectroscopy, scanning electron microscope, and monosaccharide analysis. Among fucoidans, EMSF6 was rich in fucose and sulfate and had a similar structural character to commercial fucoidan. EMSF6 showed a strong inhibitory effect on nitric oxide generation in LPS-induced RAW 264.7 cells and significantly decreased the production of LPS-induced pro-inflammatory cytokines, including interleukin-6, interleukin-1β, and tumor necrosis factor α. The anti-inflammatory potential of EMSF6 was mediated through the downregulation of inducible nitric oxide synthase and cyclooxygenase-2 expression. Thus, fucoidans from E. maxima stipe are promising candidates for functional food products.

키워드

과제정보

This research was a part of the project entitled "Discovering a process technology to develop alginic acid in mass scale for domestic uses," funded by the Ministry of Oceans and Fisheries, Korea (20220560). Further, the Korea Research Program 2022M00500 extended its support via the National Marine Biodiversity Institute of Korea.

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