BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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2-Undecanone derived from Pseudomonas aeruginosa modulates the neutrophil activity

  • Jeong, Yu Sun (Department of Biological Sciences, Sungkyunkwan University) ;
  • Huh, Sunghyun (Department of New Biology, DGIST) ;
  • Kim, Ji Cheol (Department of Biological Sciences, Sungkyunkwan University) ;
  • Park, Ji Ye (Department of Biological Sciences, Sungkyunkwan University) ;
  • Lee, ChaeEun (Department of New Biology, DGIST) ;
  • Kim, Min-Sik (Department of New Biology, DGIST) ;
  • Koo, JaeHyung (Department of New Biology, DGIST) ;
  • Bae, Yoe-Sik (Department of Biological Sciences, Sungkyunkwan University)
  • 투고 : 2022.01.10
  • 심사 : 2022.04.05
  • 발행 : 2022.08.31
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초록

Pseudomonas aeruginosa (P. aeruginosa) is a well-known Gramnegative opportunistic pathogen. Neutrophils play key roles in mediating host defense against P. aeruginosa infection. In this study, we identified a metabolite derived from P. aeruginosa that regulates neutrophil activities. Using gas chromatography-mass spectrometry, a markedly increased level of 2-undecanone was identified in the peritoneal fluid of P. aeruginosa-infected mice. 2-Undecanone elicited the activation of neutrophils in a Gαi-phospholipase C pathway. However, 2-undecanone strongly inhibited responses to lipopolysaccharide and bactericidal activity of neutrophils against P. aeruginosa by inducing apoptosis. Our results demonstrate that 2-undecanone from P. aeruginosa limits the innate defense activity of neutrophils, suggesting that the production of inhibitory metabolites is a strategy of P. aeruginosa for escaping the host immune system.

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과제정보

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2020 M3A9D3038435, NRF-2020R1A6A3A13071682, NRF-2021R 1A2C1009258), and by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI20C0026).

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