Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Population Structure of Korean Paraplagusia japonica (Cynoglossidae) Based on Morphological and Molecular Markers

한국산 흑대기 Paraplagusia japonica (참서대과)의 형태 및 분자 마커에 의한 집단구조

  • Park, Gyeong Hyun (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Jin-Koo (Division of Fisheries and Life Sciences, Pukyong National University)
  • 박경현 (국립수산과학원 생명공학과) ;
  • 김진구 (부경대학교 수산생명과학부 자원생물학전공)
  • Received : 2022.03.16
  • Accepted : 2022.04.23
  • Published : 2022.06.30
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Abstract

The cynoglossid fishes are popular for food in the world including Korea, China and Japan, and among them, Paraplagusia japonica lives all over the sea of Korea. In order to establish appropriate management measure, it is essential to clarify population structure of P. japonica from the morphological and molecular perspectives. We collected a total of 132 individuals of P. japonica from six localities in Korea between 2008 and 2021. Canonical discriminant analysis results showed that the West Sea population (Incheon) slightly differed from the South (Tongyeong, Busan) and East Sea populations (Pohang, Donghae, Sokcho). Similar results were also shown in Kruskal-Wallis test of meristic characters. Furthermore, neighbor-joining and maximum-likelihood trees based on 849 base pairs of mitochondrial DNA cytochrome b sequences showed that P. japonica was divided into two lineages (designated as A and B) with a high significance (Φst=0.0781, P<0.001). Interestingly, however, the two lineages in the admixture area (South-East Sea) were not different in morphological characters. Our results suggest that P. japonica had undergone differentiated history during the Late Pleistocene, but secondary contact may occur at the admixture area.

참서대과 어류는 한국, 중국, 일본 등 전 세계적으로 식용으로 인기가 있으며, 그중에서도 흑대기(Paraplagusia japonica)는 한국 전 해역에 서식한다. 적절한 관리방안 수립을 위해서는 형태학적, 분자적 관점에서 흑대기의 집단구조를 명확히 하는 것이 필수적이다. 본 연구에서는 2008년부터 2021년까지 국내 6개 지역에서 총 132개체의 흑대기를 채집했다. 계측 형질에서 정준판별분석(CDA) 결과 서해(인천) 집단은 남해(통영·부산)와 동해(포항·동해·속초) 집단과 약간 차이가 있는 것으로 나타났다. 계수 형질에 대한 Kruskal-Wallis test에서도 유사한 결과가 나타났다. 또한, 미토콘드리아 DNA Cytochrome b 염기서열 849 bp를 기반으로 한 neighbor-joining과 maximum-likelihood tree는 흑대기가 높은 유의성(Φst=0.0781, P<0.001)을 갖는 두 lineage (A와 B로 지정)로 나뉘어져 있음을 보여주었다. 그러나 흥미롭게도 혼합 해역(동남해)의 두 lineage는 형태학적 특징에서 유의한 차이가 없었다. 본 연구 결과는 한국산 흑대기가 플라이스토세 후기 동안 분화된 역사를 겪었으나, 혼합 해역에서 2차 접촉이 발생할 수 있다는 가능성을 시사한다.

Keywords

Acknowledgement

논문을 세심하게 검토해주신 심사위원 세 분께 감사드립니다. 또한, 시료 확보에 도움을 준 최시원, 김이정, 이유진에게도 감사의 말을 전합니다. 이 논문은 2022년 국립해양생물자원관(2022M01100)의 재원으로 수행된 연구입니다.

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