The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Inhibitory Effect of Citrus Unshiu Peel and Cimicifuga Dahurica Extracts on Melanogenesis accroding to the Various Extraction Methods

추출조건에 따른 진피(陳皮) 및 승마(升麻) 추출물의 미백 활성효과

  • Lee, Yun-Jung (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute) ;
  • Song, Hyun-Woo (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute) ;
  • Lee, Bo-Ri (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute) ;
  • Na, Hye-Rim (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute) ;
  • Song, Bong-Joon (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute) ;
  • Lee, Young-Mi (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute)
  • 이윤정 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 송현우 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 이보리 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 나혜림 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 송봉준 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소)
  • Received : 2022.07.14
  • Accepted : 2022.08.26
  • Published : 2022.08.26
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Abstract

Objectives: Excessive melanin production leads to skin pigmentation, which causes various cosmetic and health problems. Citrus unshiu Markovich peel (CS) and Cimicifuga dahurica (CD) have long been widely used as a oriental medicinal plant because of their pharmacological properties including anti-ulcer, anti-oxidant, and anti-inflammatory properties. Methods: In this study, we investigated the inhibitory effects of CS, CD extracts or CS:CD=1:2 mixture on melanogenesis according to the various extraction methods. CS and CD extracted were prepared by ethanol extraction (EE), ultrasonification extraction (USE), Supercritical extraction (SCE), reflux extraction (RE), respectively. Results: DPPH radical scavenging and tyrosinase inhibitory activity of CD extracts or CS:CD=1:2 mixture were increased in dose-dependent manners. In addition, we evaluated the effect of CS, CD extracts or CS:CD=1:2 mixture on tyrosinase activity and melanogenesis in α-melanocyte stimulating hormone-induced B16-F10 melanoma cells. CS, CD extracts or CS:CD=1:2 mixture significantly inhibited tyrosinase activity and melanogenesis at 10-200 ㎍/mL. Conclusions: Therefore, our study suggests that CS and CD extracts have potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.

Keywords

Acknowledgement

본 연구는 원광대학교 연구비지원(2022년)에 의해 수행되었음.

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