Toxicological Research

  • 제38권2호
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  • Pages.175-186
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  • 2022
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지
DOI QR코드

DOI QR Code

Trade-offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model

  • Solek, Przemyslaw (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Mytych, Jennifer (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Sujkowska, Ewelina (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Grzegorczyk, Magdalena (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Jasiewicz, Patrycja (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Sowa‑Kucma, Magdalena (Department of Human Physiology, Institute of Medical Sciences, Medical College of Rzeszow University) ;
  • Stachowicz, Katarzyna (Department of Neurobiology, Maj Institute of Pharmacology Polish Academy of Sciences) ;
  • Koziorowski, Marek (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow) ;
  • Tabecka‑Lonczynska, Anna (Department of Biotechnology, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow)
  • 투고 : 2021.01.21
  • 심사 : 2021.04.20
  • 발행 : 2022.04.15
⟨ 이전 논문 다음 논문 ⟩

초록

The increasing number of depression cases leads to a greater need for new antidepressant treatment development. It is postulated that antidepressants may harm male fertility, but the cellular mechanism is still poorly understood. The role of growth factors and klotho protein in maintaining normal male reproductive function is well documented. Hence, the study aimed to investigate the effect of the antidepressant drug - imipramine (tricyclic AD), and other substances with antidepressant potential (ALS), administered in combination or in combination with LPS (an animal model of depression) on gene expression and protein synthesis of IGF-2 (insulin-like growth factor 2), TGF-β1 (transforming growth factor β1), NGF (nerve growth factor), KGF (keratinocyte growth factor) and protein synthesis of VEGF-A (vascular endothelial growth factor A), IGF-IR (insulin-like growth factor receptor 1), EGFR (epidermal growth factor receptor) and klotho in the testis of mice. Mice were injected intraperitoneally with selected ALS and LPS or 10% DMSO (controls) (n=7/group) once a day for 14 days. Animals were decapitated and testes collected for RNA and protein purification. PCR and western blot methods were employed for the evaluation of growth factors and klotho expression. The results obtained indicated a decreased level of most of the analyzed genes and proteins, except KGF; its expression increased after treatment with MTEP and IMI administrated individually and after NS-398, and IMI in combination with LPS. Our results may suggest that the tested ALS and LPS can contribute to a reduction of male fertility, but NS-398, IMI, and IMI+NS-398 may also act as stimulants after LPS.

키워드

과제정보

This study was supported by the statutory fund of the Department of Biotechnology, Poland. Also, partially by grant No.: UMO-2014/13/D/NZ7/00292, assigned by the National Science Centre, Poland, to K. Stachowicz and by the statutory fund of the Maj Institute of Pharmacology, Polish Academy of Sciences in Krakow (Protocol No 178/2017).

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