Toxicological Research

  • 제38권2호
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  • Pages.225-233
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  • 2022
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지
DOI QR코드

DOI QR Code

Acute toxicity of aqueous extract of Ambrosia arborescens Mill. on biochemical and histopathological parameters in rats

  • Silva‑Correa, Carmen R. (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Torre, Víctor E. Villarreal‑La (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Gonzalez‑Siccha, Anabel D. (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Cruzado‑Razco, Jose L. (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Gonzalez‑Blas, Maria V. (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Sagastegui‑Guarniz, William Antonio (Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo) ;
  • Calderon‑Pena, Abhel A. (Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo) ;
  • Aspajo‑Villalaz, Cinthya L. (Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo) ;
  • Hilario‑Vargas, Julio (Facultad de Medicina, Universidad Nacional de Trujillo)
  • 투고 : 2021.05.09
  • 심사 : 2021.09.07
  • 발행 : 2022.04.15
⟨ 이전 논문 다음 논문 ⟩

초록

Medicinal plants play an important role in the management of various diseases, so their use has become widespread. However, in some cases the population uses plant species regardless of the toxicity they may possess. The objective of this study was to evaluate the acute toxicity of aqueous extract from the leaves of Ambrosia arborescens Mill. on the biochemical and histopathological parameters of albino Holtzman rats. To do this, the leaves of A. arborescens were collected in the province of Julcan, La Libertad Region-Peru. OECD (Organisation for Economic Cooperation and Development) guideline 423 was conducted, forming experimental groups of 10 animals each one (5 males and 5 females): Group I (Control), which received 2 mL physiological saline solution (SSF 0.9%), Groups II and III (A. arborescens-300 and A. arborescens-2000), which were given the aqueous extract leaves of A. arborescens in a single dose of 300 and 2000 mg/kg/day, respectively. On the 14th day of exposure, biochemical (creatinine, ALT and AST) and histopathological parameters were measured. The results show that the aqueous extract of A. arborescens at the dose of 2000 mg/kg produces an increase in biochemical parameters which is related to histopathological analysis of liver and renal tissue with mild congestion. The study concludes that the aqueous extract leaves of A. arborescens has a LD50 greater than 2000 mg/kg and produces mild congestion in kidneys and liver, but showed no significant toxicological changes in the other albino Holtzman rats organs.

키워드

참고문헌

  1. Mythilypriya R, Shanthi P, Sachdanandam P (2007) Oral acute and subacute toxicity studies with kalpaamruthaa, a modified indigenous preparation, on rats. J Heal Sci 53:351-358. https://doi.org/10.1248/jhs.53.351
  2. Yuan H, Ma Q, Ye L, Piao G (2016) The traditional medicine and modern medicine from natural products. Molecules 21:559. https://doi.org/10.3390/molecules21050559
  3. Morales G, Paredes A, Olivares A, Bravo J (2014) Acute oral toxicity and anti-inflammatory activity of hydroalcoholic extract from Lampaya medicinalis phil in rats. Biol Res 47:6. https://doi.org/10.1186/0717-6287-47-6
  4. Manekeng HT, Mbaveng AT, Ntyam Mendo SA, Agokeng AJD, Kuete V (2019) Evaluation of acute and subacute toxicities of Psidium guajava methanolic bark extract: a botanical with in vitro antiproliferative potential. Evid Based Complement Altern Med 2019:8306986. https://doi.org/10.1155/2019/8306986
  5. Vaghasiya YK, Shukla VJ, Chanda SV (2010) Acute oral toxicity study of Pluchea arguta boiss extract in mice. J Pharmacol Toxicol 6:113-123. https://doi.org/10.3923/jpt.2011.113.123
  6. Li Y, Kandhare AD, Mukherjee AA, Bodhankar SL (2019) Acute and sub-chronic oral toxicity studies of hesperidin isolated from orange peel extract in Sprague Dawley rats. Regul Toxicol Pharmacol 105:77-85. https://doi.org/10.1016/j.yrtph.2019.04.001
  7. Han C-T, Kim M-J, Moon S-H, Jeon Y-R, Hwang J-S, Nam C et al (2015) Acute and 28-day subacute toxicity studies of hexane extracts of the roots of Lithospermum erythrorhizon in Sprague-Dawley rats. Toxicol Res 31:403-414. https://doi.org/10.5487/TR.2015.31.4.403
  8. de Araujo MC PM, Barcellos NMS, de Vieira PM A, Gouveia TM, Guerra MO, Peters VM et al (2017) Acute and sub chronic toxicity study of aqueous extract from the leaves and branches of Campomanesia velutina (Cambess) O Berg. J Ethnopharmacol 201:17-25. https://doi.org/10.1016/j.jep.2017.02.043
  9. Aydin A, Aktay G, Yesilada E (2016) A guidance manual for the toxicity assessment of traditional herbal medicines. Nat Prod Commun 11:1763-1773. https://doi.org/10.1177/1934578X1601101131
  10. Han JS, Lee BS, Han SR, Han HY, Chung MK, Min BS et al (2016) A subchronic toxicity study of Radix Dipsaci water extract by oral administration in F344 rats. Regul Toxicol Pharmacol 81:136-145. https://doi.org/10.1016/j.yrtph.2016.07.017
  11. Balogun FO, Tom Ashafa AO (2016) Acute and subchronic oral toxicity evaluation of aqueous root extract of Dicoma anomala Sond. in Wistar Rats. Evid Based Complement Altern Med 2016:3509323. https://doi.org/10.1155/2016/3509323
  12. Hazarika I, Geetha KM, Sundari PS, Madhu D (2019) Acute oral toxicity evaluation of extracts of Hydrocotyle sibthorpioides in wistar albino rats as per OECD 425 TG. Toxicol Reports 2019:321-328. https://doi.org/10.1016/j.toxrep.2019.04.001
  13. Neergheen-Bhujun VS (2013) Underestimating the toxicological challenges associated with the use of herbal medicinal products in developing countries. Biomed Res Int 2013:804086. https://doi.org/10.1155/2013/804086
  14. Saad B, Azaizeh H, Abu-Hijleh G, Said O (2006) Safety of traditional Arab herbal medicine. Evid Based Complement Altern Med 3:433-439. https://doi.org/10.1093/ecam/nel058
  15. Kognou ALM, Tchamgoue AD, Tchokouaha LRY, Nthenge-Ngumbau DN, Fokou PVT, Tchinda AT et al (2018) Acute and sub-chronic toxicity studies of Dichaetanthera africana (Hook. F.) Jacq. Fel. (Melastomataceae) stem bark ethanol extract. J Appl Pharm Sci 8:147-155. https://doi.org/10.7324/JAPS.2018.8619
  16. Al-Afifi NA, Alabsi AM, Bakri MM, Ramanathan A (2018) Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats. BMC Complement Altern Med 18:50. https://doi.org/10.1186/s12906-018-2110-3
  17. Yuet Ping K, Darah I, Chen Y, Sreeramanan S, Sasidharan S (2013) Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. Biomed Res Int 2013:182064. https://doi.org/10.1155/2013/182064
  18. Cano de Terrones T (2014) Caracterizacion de una espirolactona sesquiterpenica α-metilenica obtenida de Ambrosia arborescens Miller y evaluacion de su actividad biologica en Trypanosoma cruzi. Rev la Soc Quimica del Peru 80:124-135. https://doi.org/10.37761/rsqp.v80i2.157
  19. Ruiz C, Diaz C, Rojas R (2015) Composicion Quimica de Aceites Esenciales de 10 Plantas Aromaticas Peruanas. Rev la Soc Quimica del Peru 81:81-94. https://doi.org/10.37761/rsqp.v81i2.10
  20. De Leo M, Saltos MBV, Puente BFN, De Tommasi N, Braca A (2010) Sesquiterpenes and diterpenes from Ambrosia arborescens. Phytochemistry 71:804-809. https://doi.org/10.1016/j.phytochem.2010.02.002
  21. Villagomez R, Rodrigo GC, Collado IG, Calzado MA, Munoz E, Akesson B et al (2013) Multiple anticancer effects of damsin and coronopilin isolated from Ambrosia arborescens on cell cultures. Anticancer Res 33:3799-3806 (PMID: 24023312). https://ar.iiarjournals.org/content/anticanres/33/9/3799.full.pdf
  22. Sotillo WS, Villagomez R, Smiljanic S, Huang X, Malakpour A, Kempengren S et al (2017) Anti-cancer stem cell activity of a sesquiterpene lactone isolated from Ambrosia arborescens and of a synthetic derivative. PLoS ONE 12:e0184304. https://doi.org/10.1371/journal.pone.0184304
  23. Das B, Dasi R (1995) Chemical investigation in Parthenium hysteruphorus-an allelopathic plant. Allelopathy J 2:99-104. https://www.allelopathyjournal.com/Journal_Articles/AJ%2%(1)%January,%1995%(99-104).pdf 104).pdf
  24. Aregullin M, Rodriguez E (2003) Sesquiterpene Lactone Sequestration by the Tortoise Beetle Physonota arizonae (Cassidinae). Rev Soc Quim Mex 47:143-145. http://www.scielo.org.mx/pdf/rsqm/v47n2/v47n2a11.pdf
  25. Teves MR, Matera S, Consolini AE, Wendel GH (2020) Effect of the leaves aqueous extract of Jodina rhombifolia (Hook. & Arn.) Reissek (Santalaceae) on intestinal function and its acute toxicity. J Ethnopharmacol 262:113205. https://doi.org/10.1016/j.jep.2020.113205
  26. Sotiropoulou NS, Megremi SF, Tarantilis P (2020) Evaluation of antioxidant activity, toxicity, and phenolic profile of aqueous extracts of chamomile (Matricaria chamomilla L.) and sage (Salvia ocinalis L.) prepared at different temperatures. Appl Sci 10:2270. https://doi.org/10.3390/app10072270
  27. The Organization for Economic Co-operation and Development (OECD). OECD guideline for testing of chemicals: acute oral toxicity-acute toxic class method (OECD 423). https://www.oecd.org/chemicalsafety/testing/45124172.pdf. Accessed 15 Apr 2021
  28. Singha CH, Nath K, Kumar P, Kumar A, Dixit J, Saini R et al (2021) Toxicity profiling and antioxidant activity of ethyl acetate extract of leaves of Premna integrifolia L. for its application as protective agent against xenobiotics. Toxicol Rep 8:196-205. https://doi.org/10.1016/j.toxrep.2021.01.004
  29. Committee for the Update of the Guide for the Care and Use of Laboratory Animals (2011) Guide for the care and use of laboratory animals, 8th edn. The National Academies Press, Washington
  30. Instituto Nacional de Salud (2008) Guia de manejo y cuidado de animales de laboratorio: raton. https://repositorio.ins.gob.pe/xmlui/bitstream/handle/INS/117/CNPB-0002.pdf?sequence=3&isAllowed=y. Accessed 15 Apr 2021
  31. Rojas-Armas J, Arroyo-Acevedo J, Ortiz-Sanchez M, Palomino-Pacheco M, Castro-Luna A, Ramos-Cevallos N et al (2019) Acute and repeated 28-day oral dose toxicity studies of Thymus vulgaris L. essential oil in rats. Toxicol Res 35:225-232. https://doi.org/10.5487/TR.2019.35.3.225
  32. Ribeiro NE, Pereira PS, de Oliveira TB, de Arruda Lima SM, Silva TMS, Santana ALBD et al (2020) Acute and repeated dose 28-day oral toxicity of Chrysobalanus icaco L. leaf aqueous extract. Regul Toxicol Pharmacol 113:104643. https://doi.org/10.1016/j.yrtph.2020.104643
  33. Kim HY, Zuo G, Lee SK, Lim SS (2020) Acute and subchronic toxicity study of nonpolar extract of licorice roots in mice. Food Sci Nutr 8:2242-2250. https://doi.org/10.1002/fsn3.1465
  34. Syahirah S, Hamzah H, Rosly M, Farhan M, Mohamed N, Norhaizan E (2018) Subacute oral toxicity assesment of ethanol extract of Mariposa christia vespertilionis leaves in male Sprague Dawley rats. Toxicol Res 34:85-95. https://doi.org/10.5487/TR.2018.34.2.085
  35. El Kabbaoui M, Chda A, El-Akhal J, Azdad O, Mejrhit N, Aarab L et al (2017) Acute and sub-chronic toxicity studies of the aqueous extract from leaves of Cistus ladaniferus L. in mice and rats. J Ethnopharmacol 209:147-156. https://doi.org/10.1016/j.jep.2017.07.029
  36. Atsafack SS, Kuiate JR, Mouokeu RS, Koanga Mogtomo ML, Tchinda AT, De Dieu TJ et al (2015) Toxicological studies of stem bark extract from Schefflera barteri Harms (Araliaceae). BMC Complement Altern Med 15:44. https://doi.org/10.1186/s12906-015-0581-z
  37. Hassanzadeh-Taheri M, Hosseini M, Salimi M, Moodi H, Dorranipour D (2018) Acute and sub-acute oral toxicity evaluation of Astragalus hamosus seedpod ethanolic extract in Wistar rats. Pharm Sci 24:23-30. https://doi.org/10.15171/PS.2018.05
  38. Ndrepepa G (2021) Aspartate aminotransferase and cardiovascular disease-a narrative review. J Lab Precis Med 6:6. https://doi.org/10.21037/jlpm-20-93
  39. Amadike E, Emmanuel I, Chibueze V, Gilbert J, Ekeanyanwu B (2019) Toxicological evaluation of phytochemical characterized aqueous extract of wild dried Lentinus squarrosulus (Mont.) mushroom in rats. Toxicol Res 35:181-190. https://doi.org/10.5487/TR.2019.35.2.181
  40. Amacher D (2013) Female gender as a susceptibility factor for drug-induced liver injury. Hum Exp Toxicol 33:928-939. https://doi.org/10.1177/0960327113512860
  41. Chebaibi M, Bousta D, Chbani L, Ez zoubi Y, Touiti N, Achour S (2019) Acute toxicity of plants mixture used in traditional treatment of edema and colic renal in Morocco. Sci Afr 6:e00152. https://doi.org/10.1016/j.sciaf.2019.e00152
  42. Kifayatullah M, Shahimi M, Sengupta P, Rahman M, Das A, Das S (2015) Evaluation of the acute and sub-acute toxicity of the ethanolic extract of Pericampylus glaucus (Lam.) Merr. in BALB/c mice. J Acute Dis 4:309-315. https://doi.org/10.1016/j.joad.2015.06.010