Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Ameliorative or corrective effects of Fig "Ficus carica" extract on nickel-induced hepatotoxicity in Wistar rats

  • Nemiche, Souhila (Laboratory of Experimental Bio-Toxicology, Bio-Depollution and Phyto-Remediation, Department of Biology, Faculty of Nature and Life Sciences, University of Oran 1 Ahmed Ben Bella) ;
  • Hamadouche, Nadia Ait (Laboratory of Experimental Bio-Toxicology, Bio-Depollution and Phyto-Remediation, Department of Biology, Faculty of Nature and Life Sciences, University of Oran 1 Ahmed Ben Bella) ;
  • Nemmiche, Said (Department of Biology, Faculty of Nature and Life Sciences, University of Mostaganem) ;
  • Fauconnier, Marie‑Laure (Laboratory of General and Organic Chemistry, University of Liege, Gembloux Agro-Bio Tech) ;
  • Tou, Abdenacer (Service of Pathology, University Hospital of Sidi Bel Abbes)
  • Received : 2021.09.03
  • Accepted : 2021.11.30
  • Published : 2022.07.15
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Abstract

Many heavy metals and metalloids (e.g., Pb, Cd, and Ni) can contaminate the environment and cause severe health problems. Through this study, investigated the possible corrective effects of Ficus carica extract (FCE) against nickel (Ni) induced stress response and damage on the liver of rats. Male Wistar rats were divided into four groups (8 rats per group) and co-treated with FCE (350 mg/kg) and exposed to Nickel chloride (10 mg/kg) for 4 weeks. The volatile compounds of FCE were characterized by solid phase micro-extraction (SPME) coupled with GC-MS, and the biochemical parameters of stress were determined. The SPME-GC/MS analysis of FCE indicated the presence of thirty (30) phyto-bioactive compounds including alcohols, aldehydes, organic acids, ketones, furans, terpenes, ester and others. The best capacity for scavenging DPPH free radicals and metal chelating were found with the IC50 values of 0.49 and 2.91 mg/mL, respectively. Ni induced damage to various macromolecules. Malondialdehyde, protein carbonyls, alanine aminotransferase and gamma glutamyl transferarse levels were significantly increased in Ni exposed group compared to control group and co-treatment with FCE reduced the levels of these parameters. In conclusion, current findings showed that Ni-induced oxidative damage and the administration of FCE can improve correct and restore the alteration in the rat liver.

Keywords

References

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