Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-inflammation and Anti-aging Effect of Lactic Acid Bacteria Fermented Lycium chinense Extracts

젖산균 발효 구기자(Lycium chinense ) 추출물의 항염증 및 항노화 효능 연구

  • Han, Dong-Geun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Lee, Ju-Seong (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Gal, Gyeok (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Park, Nam-Wan (R&D Center, Nature Botanics.Co., Ltd.) ;
  • Kim, Hyun-Jeong (R&D Center, HONEST.Co., Ltd.) ;
  • An, Bong-Jeon (Department of Cosmeceutical Science, Daegu Hanny University)
  • 한동근 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 이주성 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 갈격 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 박남완 ((주)네이처보타닉스) ;
  • 김현정 ((주)허니스트) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Received : 2022.05.25
  • Accepted : 2022.06.16
  • Published : 2022.06.30
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Abstract

In this study, fermented Lycium chinense (L. chinense) extract was used to analyze the efficacy of nitric oxide (NO) and anti-aging inhibition to verify its usefulness as a functional cosmetic material. L. chinense was extracted with hydrothermal and 70% ethanol as a solvent, fermented, concentrated, and freeze-dried to prepare a sample, followed by MTT assay, NO inhibition assay, western blot assay, and UPLC analysis.As a result of the analysis of NO inhibitory efficacy, fermented L. chinense water extract (FLW) and fermented L. chinense 70% ethanol extract (FLE) showed excellent NO inhibitory efficacy of 47.96% and 56.71%, respectively. As a result of measuring the expression patterns of the wrinkle-related proteins MMP-1, and TRPV-1 through Western blot, both FLW and FLE confirmed the inhibitory efficacy of MMP-1 and TRPV-1. Based on the results of the experiment, the fermented L. chinense extract is expected to have a high application value as a functional cosmetic material related anti-inflammatory, and anti-aging.

본 연구에서는 발효 과정을 거친 구기자(Lycium chinense) 추출물을 이용하여 항염증 및 항노화 관련 기능성 화장품 소재로서의 활용가치를 검증하고자 nitric oxide (NO) 및 항노화 관련 인자들의 억제 효능을 분석하였다. 물과 70% 에탄올을 용매로 구기자를 추출, 발효, 농축, 동결건조하여 시료를 제조한 다음, MTT assay, NO inhibition activity assay, western blot assay, UPLC 분석을 진행하였다. MTT assay를 통해 세포독성을 측정한 다음, 500 ㎍/mL의 농도를 세포실험에 적용하여 진행하였다. NO 억제 효능의 분석 결과, 발효 구기자 열수 추출물(FLW), 발효 구기자 70% 에탄올 추출물(FLE) 각각 47.96%, 56.71%의 우수한 NO 억제 효능을 나타내었다. Western blot을 통해 주름 관련 단백질인 MMP-1, TRPV-1의 발현양상을 측정한 결과, FLW, FLE 모두 MMP-1, TRPV-1에 대한 발현 억제 효능을 나타내었다. 따라서 발효 구기자 추출물은 항염증 및 항노화 관련 기능성 화장품 소재로서의 높은 활용 가치가 있을 것으로 판단된다.

Keywords

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