Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Development and Validation of an Analytical Method for β-Agonists in Livestock and Fishery Products Using LC-MS/MS

LC-MS/MS를 이용한 축·수산물 중 β-agonist계 시험법 개발 및 검증

  • Lee, Tae Ho (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety) ;
  • Kim, Yu Ra (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety) ;
  • Park, Su Jeong (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety) ;
  • Kim, Ji Young (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety) ;
  • Choi, Jang Duck (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety) ;
  • Moon, Gui Im (Pesticide and Veterinary Drug Residues Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety)
  • 이태호 (식품의약품안전평가원 위해평가부 잔류물질과) ;
  • 김유라 (식품의약품안전평가원 위해평가부 잔류물질과) ;
  • 박수정 (식품의약품안전평가원 위해평가부 잔류물질과) ;
  • 김지영 (식품의약품안전평가원 위해평가부 잔류물질과) ;
  • 최장덕 (식품의약품안전평가원 위해평가부 잔류물질과) ;
  • 문귀임 (식품의약품안전평가원 위해평가부 잔류물질과)
  • Received : 2022.06.15
  • Accepted : 2022.06.23
  • Published : 2022.06.30
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Abstract

BACKGROUND: The β-agonists known as phenyl ethanolamine derivatives have a conjugated aromatic ring with amino group. They are used as tocolytic agents and bronchodilator to human and animal generally, and some of them are used as growth promoters to livestock. METHODS AND RESULTS: β-agonists in samples were extracted by 0.4 N perchloric acid and ethyl acetate. The target compounds were analyzed by liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS). Validation of method was performed according to CODEX guidelines (CAC/GL-71). The matrix matched calibration gave correlation coefficients>0.98, and the obtained recoveries were in the range of 62.0-109.8%, with relative standard deviation ≤ 20.1%. In addition, a survey was performed to inspect any residual β-agonist from 100 samples of livestock and fishery products and ractopamine was detected in one of the 100 samples. CONCLUSION(S): In this study, we established the analytical method for β-agonists through using the expanded target compounds and samples. And we anticipate that the established method would be used for analysis to determine veterinary drug residues in livestock and fishery products.

Keywords

Acknowledgement

This study was carried out with support of National Institute of Food and Drug Safety Evaluation of Republic of Korea (project no. 20161MFDS603).

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