식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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수박에 과실썩음병을 유발하는 Acidovorax citrulli의 생물학적 방제를 위한 길항 미생물 선발과 특성 검정

Selection and Characterization of Antagonistic Microorganisms for Biological Control of Acidovorax citrulli Causing Fruit Rot in Watermelon

  • 김기영 (강원대학교 식물자원응용공학과) ;
  • 박효빈 (강원대학교 식물자원응용공학과) ;
  • ;
  • 김현승 (강원대학교 식물자원응용공학과) ;
  • 변은정 (강원대학교 식물자원응용공학과) ;
  • 이인규 (강원대학교 식물자원응용공학과) ;
  • 이윤수 (강원대학교 식물자원응용공학과)
  • Kim, Ki Young (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Park, Hyo Bin (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Adhikari, Mahesh (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kim, Hyun Seung (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Byeon, Eun Jeong (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Lee, In Kyu (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Lee, Youn Su (Department of Applied Plant Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University)
  • 투고 : 2022.01.21
  • 심사 : 2022.06.15
  • 발행 : 2022.06.30
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초록

본 연구에서는 수박에서 과실썩음병을 유발하는 Acidovorax citrulli를 억제하는 세균을 선발하고, 선발한 세균이 성장촉진 및 길항 효소를 생성하는지 확인하였다. 전국 26개 지역의 94곳의 수박재배지와 25곳의 대형 원예 육묘장에서 수박 식물체(잎, 꽃, 뿌리) 및 토양을 수집하였다. 수집한 sample에서 tryptic soy agar와 yeast extract peptone dextrose 배지에서 각각 1,953종과 841종 총 2,794종의 미생물을 분리하였으며, 2,794종의 미생물 중 2종의 A. citrulli를 선발하였다. 선발한 A. citrulli에 대한 기내 길항성 검정 결과 3-3B에서 24종, 9-4B에서 14종 총 28종의 길항 세균을 선발하였다. 선발한 길항 세균 중 BNPL-6-3B, HYGPL-1-3B, TIPL-6-1B, YGMP-2-7Y는 2종의 bacterial fruit blotch균 모두에서 강한 길항성을 보였다. 선발한 28종의 길항 세균을 대상으로 총 6가지의 생화학적 검증을 진행하였으며, 선발한 길항 세균 중 CB20R-2-5B, TIPL-4-2B, TIPL-6-1B, TIPL-7-4B 4가지 균주가 5가지의 생화학적 검증에서 성장촉진 및 길항 효소를 분비하는 것을 확인하였다. 또한, BNPL-3-3B와 BNPL-6-3B 두 균주는 성장촉진 효소 분비를 확인하는 4가지의 생화학적 검증(ammonia production, phosphate solubilization, starch hydrolysis, siderophore production)에서 모두에서 효소를 분비하는 것으로 확인되었다. 본 연구에서 선발한 28종의 길항 세균은 배지에서 A. citrulli를 효과적으로 억제하였으며, 사이안화 수소와 단백질분해효소 생산 능력 검정을 통해 길항 활성을 확인하였다. 또한, 선발한 길항 세균의 암모니아 생산 및 불용성 인산 가용화 능력을 확인하여 식물 성장촉진 활성을 가지는 것으로 확인하였다. 따라서, 본 연구결과는 향후 수박 과실썩음병 방제제 및 성장촉진제 등의 친환경 농자재 개발에 유용한 기초 자료가 될 것으로 판단된다.

This study was performed to screen the efficacy of antagonistic bacterial isolates from various sources against the bacterial fruit blotch (BFB) causing pathogen (Acidovorax citrulli) in cucurbit crops. In addition, plant growth promoting traits of these antagonistic bacterial isolates were characterized. Two thousand seven hundred ninety-four microorganisms were isolated from the collected samples. Molecular identification revealed two A. citrulli out of 2,794 isolates. In vitro antagonistic results showed that, among the 28 antagonistic bacterial isolates, 24 and 14 bacterial isolates exhibited antagonism against HPP-3-3B and HPP-9-4B, respectively. Antagonistic and growth promotion characterization of the antagonistic bacterial isolates were further studied. Results suggested that, 4 antagonistic bacteria commonly showed both antagonism and growth promotion phenotypes. Moreover, 3 isolates possessed growth promoting activities. Overall results from this study suggests that BFB causing bacterial pathogen (A. citrulli) was suppressed in in vitro antagonism assay by antagonistic bacterial isolates. Furthermore, these antagonistic bacterial isolates possessed growth promotion and antagonistic enzyme production ability. Therefore, data from this study can provide useful basic data for the in vivo experiments which ultimately helps to develop the eco-friendly agricultural materials to control fruit rot disease in cucurbit crops in near future.

키워드

과제정보

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET), through Development of new control methods for bacterial fruit rots through analysis of the pathogenicity of the pathogen (120088-05-2). This research has been worked with the support of a research grant of Kangwon National University in 2021.

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