BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Inhibition of ClC-5 suppresses proliferation and induces apoptosis in cholangiocarcinoma cells through the Wnt/β-catenin signaling pathway

  • Shi, Zhe (Department of General Surgery, Affiliated Hospital of Hebei Engineering University) ;
  • Zhou, Liyuan (Department of Gynaecology, Affiliated Hospital of Hebei Engineering University) ;
  • Zhou, Yan (Department of Nursing, Medical College, Hebei University of Engineering) ;
  • Jia, Xiaoyan (Department of General Surgery, Affiliated Hospital of Hebei Engineering University) ;
  • Yu, Xiangjun (Department of General Surgery, Affiliated Hospital of Hebei Engineering University) ;
  • An, Xiaohong (Department of Hospital Infection-Control, Jize County People's Hospital) ;
  • Han, Yanzhen (Department of General Surgery, Affiliated Hospital of Hebei Engineering University)
  • 투고 : 2022.03.07
  • 심사 : 2022.04.28
  • 발행 : 2022.06.30
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초록

Chloride channel-5 (ClC-5), an important branch of the ClC family, is involved in the regulation of the proliferation and cell-fate of a variety of cells, including tumor cells. However, its function in cholangiocarcinoma (CCA) cells remains enigmatic. Here, we discovered that ClC-5 was up-regulated in CCA tissues and CCA cell lines, while ClC-5 silencing inhibited CCA cell proliferation and induced apoptosis. Further mechanism studies revealed that ClC-5 inhibition could inhibit Wnt/β-catenin signaling activity and further activate the mitochondria apoptotic pathway in CCA cells. Furthermore, rescuing Wnt/β-catenin signaling activation eliminated the anti-tumor function of ClC-5 knockdown. Together, our research findings illustrated that ClC-5 inhibition plays an anti-tumor role in CCA cells via inhibiting the activity of the Wnt/β-catenin pathway, which in turn activates the mitochondrial apoptotic pathway.

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과제정보

This work was supported by the Scientific research project of Hebei Administration of Traditional Chinese Medicine (2020227).

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