Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Effects of dietary by-products discarded after probiotics production (BPPP) on growth performance, innate immunity, immune gene expression, and disease resistance against Edwardsiella tarda in carp, Cyprinus carpio

유산균 생산 후 폐기되는 부산물 첨가 사료의 급이가 잉어(Cyprinus carpio)의 성장률, 선천성 면역, 면역연관 유전자 발현 및 항균효과에 미치는 영향

  • Choi, Jae Hyeok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Jung, Sang Mok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Yang, Eun Chong (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Jang, Tae Won (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Lee, Chan Heun (Geum Sung Sang Gong co., Ltd.) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Choi, Sanghoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 최재혁 (군산대학교 해양과학대학 수산생명의학과) ;
  • 정상목 (군산대학교 해양과학대학 수산생명의학과) ;
  • 양은총 (군산대학교 해양과학대학 수산생명의학과) ;
  • 장태원 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이찬흔 ((유)금성상공) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2022.05.26
  • Accepted : 2022.06.09
  • Published : 2022.06.30
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Abstract

This study has been performed to investigate the potential effects of by-product discarded after probiotics production (BPPP) on growth performance, immune gene expression, innate-immunity status, and disease resistance of carp, Cyprinus carpio. For 3 weeks, carp were fed four diets containing different levels of BPPP at 0, 0.1, 0.2 and 0.5% per kg of normal diet. Every 7 days of feeding, immune-related gene expression, serum lysozyme activity and ACH50 were analyzed. Growth rates and challenge test with E. tarda were conducted after 3 weeks of BPPP feeding. Both lysozyme activity and ACH50 were significantly (p<0.05) increased in all BPPP supplemented groups compared to the control at every 7 day for 3 weeks of feeding trial. The gene expression of pro-inflammatory cytokines, IL-1β and TNF-α was significantly (p<0.05) up-regulated until 21 days of feeding in all groups except for 0.2% group on day 7 post feeding. The anti-inflammatory cytokine IL-10 gene expression was only significantly (p<0.05) increased in 0.1% group on day 7 and decreased (p<0.05) on day 14 in all BPPP supplemented groups. On day 21, the IL-10 gene expression was augmented (p<0.05) in all groups. SOD gene expression was significantly (p<0.05) increased compared to the control on day 14 and 21 post feeding, whereas no significant difference was observed on day 7. In challenging test, 0.2%, 0.1%, 0.5% and control group showed 80%, 70%, 60% and 40% of survival rate, respectively. Feed conversion rate was only improved in 0.5% group. In conclusion, the present study indicates that dietary BPPP suplementation improved growth performance, innate immune response and bactericidal activity in carp.

Keywords

Acknowledgement

본 논문은 2022학년도 군산대학교 수산과학연구소 학술경비로 지원되었음.

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