한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제35권3호
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  • Pages.204-212
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  • 2022
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

함유황 다당체 Fucoidan의 인체 대장암세포(HT-29) 사멸과 Apoptosis에 미치는 영향

Effects of Fucoidan, a Sulfur-Containing Polysaccharide, on Cytotoxicity and Apoptosis in HT-29 Human Colorectal Cancer Cells

  • 김민지 (덕성여자대학교 과학기술대학 식품영양학과) ;
  • 정하숙 (덕성여자대학교 과학기술대학 식품영양학과)
  • Kim, Min Ji (Dept. of Food and Nutrition, College of Science and Technology, Duksung Women's University) ;
  • Chung, Ha Sook (Dept. of Food and Nutrition, College of Science and Technology, Duksung Women's University)
  • 투고 : 2022.03.18
  • 심사 : 2022.06.12
  • 발행 : 2022.06.30
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초록

The purpose of this study was to investigate the biological activity of fucoidan, a sulfur-containing polysaccharide, on cytotoxicity and apoptosis in the human HT-29 colorectal cancer cell line using cell viability, Flow cytometry, Western blot, and RT-PCR analyses. Fucoidan inhibited the proliferation of HT-29 cells by 39.6% at a concentration of 100 ㎍/mL for 72 h. The inhibition was dose-dependent and accompanied by apoptosis. Flow cytometric analysis showed that fucoidan increased early apoptosis and late apoptosis by 65.84% and 72.09% at concentrations of 25 and 100 ㎍/mL, respectively. Analysis of the mechanism of these events indicated that fucoidan-treated cells exhibited increases in the activation of caspase-3, caspase-8, and PARP in a dose-dependent manner. These results suggest that fucoidan may inhibit the growth of human colorectal cancer cells by various apoptosis-promoting effects, as well as by apoptosis itself.

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