Korean Journal of Radiology

  • 제23권4호
  • /
  • Pages.446-454
  • /
  • 2022
  • /
  • 1229-6929(pISSN)
  • /
  • 2005-8330(eISSN)
대한영상의학회 (The Korean Society of Radiology)
권호 표지
DOI QR코드

DOI QR Code

Hyperoxia-Induced ΔR1: MRI Biomarker of Histological Infarction in Acute Cerebral Stroke

  • Kye Jin Park (Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Ji-Yeon Suh (Asan Institute for Medical Sciences, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Changhoe Heo (Asan Institute for Medical Sciences, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Miyeon Kim (Asan Institute for Medical Sciences, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Jin Hee Baek (Asan Institute for Medical Sciences, University of Ulsan College of Medicine, Asan Medical Center) ;
  • Jeong Kon Kim (Department of Radiology and Research Institute of Radiology, University of Ulsan College of Medicine, Asan Medical Center)
  • 투고 : 2021.06.25
  • 심사 : 2021.11.25
  • 발행 : 2022.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: To evaluate whether hyperoxia-induced ΔR1 (hyperO2ΔR1) can accurately identify histological infarction in an acute cerebral stroke model. Materials and Methods: In 18 rats, MRI parameters, including hyperO2ΔR1, apparent diffusion coefficient (ADC), cerebral blood flow and volume, and 18F-fluorodeoxyglucose uptake on PET were measured 2.5, 4.5, and 6.5 hours after a 60-minutes occlusion of the right middle cerebral artery. Histological examination of the brain was performed immediately following the imaging studies. MRI and PET images were co-registered with digitized histological images. The ipsilateral hemisphere was divided into histological infarct (histological cell death), non-infarct ischemic (no cell death but ADC decrease), and nonischemic (no cell death or ADC decrease) areas for comparisons of imaging parameters. The levels of hyperO2ΔR1 and ADC were measured voxel-wise from the infarct core to the non-ischemic region. The correlation between areas of hyperO2ΔR1-derived infarction and histological cell death was evaluated. Results: HyperO2ΔR1 increased only in the infarct area (p ≤ 0.046) compared to the other areas. ADC decreased stepwise from non-ischemic to infarct areas (p = 0.002 at all time points). The other parameters did not show consistent differences among the three areas across the three time points. HyperO2ΔR1 sharply declined from the core to the border of the infarct areas, whereas there was no change within the non-infarct areas. A hyperO2ΔR1 value of 0.04 s-1 was considered the criterion to identify histological infarction. ADC increased gradually from the infarct core to the periphery, without a pronounced difference at the border between the infarct and non-infarct areas. Areas of hyperO2ΔR1 higher than 0.04 s-1 on MRI were strongly positively correlated with histological cell death (r = 0.862; p < 0.001). Conclusion: HyperO2ΔR1 may be used as an accurate and early (2.5 hours after onset) indicator of histological infarction in acute stroke.

키워드

과제정보

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2017R1A2B3007567) and through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (HI14C1090).

참고문헌

  1. Zhang YB, Su YY, He YB, Liu YF, Liu G, Fan LL. Early neurological deterioration after recanalization treatment in patients with acute ischemic stroke: a retrospective study. Chin Med J (Engl) 2018;131:137-143
  2. Li F, Liu KF, Silva MD, Meng X, Gerriets T, Helmer KG, et al. Acute postischemic renormalization of the apparent diffusion coefficient of water is not associated with reversal of astrocytic swelling and neuronal shrinkage in rats. AJNR Am J Neuroradiol 2002;23:180-188
  3. Leigh R, Knutsson L, Zhou J, van Zijl PC. Imaging the physiological evolution of the ischemic penumbra in acute ischemic stroke. J Cereb Blood Flow Metab 2018;38:1500-1516
  4. Shen Q, Du F, Huang S, Duong TQ. Spatiotemporal characteristics of postischemic hyperperfusion with respect to changes in T1, T2, diffusion, angiography, and blood-brain barrier permeability. J Cereb Blood Flow Metab 2011;31:2076-2085
  5. Colliez F, Safronova MM, Magat J, Joudiou N, Peeters AP, Jordan BF, et al. Oxygen mapping within healthy and acutely infarcted brain tissue in humans using the nmr relaxation of lipids: a proof-of-concept translational study. PLoS One 2015;10:e0135248
  6. O'Connor JP, Boult JK, Jamin Y, Babur M, Finegan KG, Williams KJ, et al. Oxygen-enhanced mri accurately identifies, quantifies, and maps tumor hypoxia in preclinical cancer models. Cancer Res 2016;76:787-795
  7. Suh JY, Cho G, Song Y, Woo DC, Choi YS, Ryu EK, et al. Hyperoxia-Induced ΔR1. Stroke 2018;49:3012-3019
  8. Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989;20:84-91
  9. Popp A, Jaenisch N, Witte OW, Frahm C. Identification of ischemic regions in a rat model of stroke. PLoS One 2009;4:e4764
  10. Zille M, Farr TD, Przesdzing I, Muller J, Sommer C, Dirnagl U, et al. Visualizing cell death in experimental focal cerebral ischemia: promises, problems, and perspectives. J Cereb Blood Flow Metab 2012;32:213-231
  11. Li H, Zhang N, Lin HY, Yu Y, Cai QY, Ma L, et al. Histological, cellular and behavioral assessments of stroke outcomes after photothrombosis-induced ischemia in adult mice. BMC Neurosci 2014;15:58
  12. Liu F, Lu J, Manaenko A, Tang J, Hu Q. Mitochondria in ischemic stroke: new insight and implications. Aging Dis 2018;9:924-937
  13. Yang JL, Mukda S, Chen SD. Diverse roles of mitochondria in ischemic stroke. Redox Biol 2018;16:263-275
  14. Crack PJ, Taylor JM. Reactive oxygen species and the modulation of stroke. Free Radic Biol Med 2005;38:1433-1444
  15. Singhal AB, Benner T, Roccatagliata L, Koroshetz WJ, Schaefer PW, Lo EH, et al. A pilot study of normobaric oxygen therapy in acute ischemic stroke. Stroke 2005;36:797-802
  16. Wintermark M, Albers GW, Broderick JP, Demchuk AM, Fiebach JB, Fiehler J, et al. Acute stroke imaging research roadmap II. Stroke 2013;44:2628-2639
  17. Butcher KS, Parsons MW, Davis S, Donnan G. PWI/DWI mismatch: better definition required. Stroke 2003;34:e215-e216
  18. Kidwell CS. MRI biomarkers in acute ischemic stroke: a conceptual framework and historical analysis. Stroke 2013;44:570-578
  19. Bateman M, Slater LA, Leslie-Mazwi T, Simonsen CZ, Stuckey S, Chandra RV. Diffusion and perfusion mr imaging in acute stroke: clinical utility and potential limitations for treatment selection. Top Magn Reson Imaging 2017;26:77-82
  20. Ding Z, Tong WC, Lu XX, Peng HP. Hyperbaric oxygen therapy in acute ischemic stroke: a review. Interv Neurol 2014;2:201-211
  21. Rusyniak DE, Kirk MA, May JD, Kao LW, Brizendine EJ, Welch JL, et al. Hyperbaric oxygen therapy in acute ischemic stroke: results of the hyperbaric oxygen in acute ischemic stroke trial pilot study. Stroke 2003;34:571-574
  22. Jiang K, Zhu Y, Jia S, Wu Y, Liu X, Chung YC. Fast T1 mapping of the brain at high field using Look-Locker and fast imaging. Magn Reson Imaging 2017;36:49-55
  23. Pastor G, Jimenez-Gonzalez M, Plaza-Garcia S, Beraza M, Reese T. Fast T1 and T2 mapping methods: the zoomed U-FLARE sequence compared with EPI and snapshot-FLASH for abdominal imaging at 11.7 tesla. MAGMA 2017;30:299-307