Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Characterization of ovarian culture in vitro and sex steroids in vivo by recombinant eel gonadotropin treatments in the eel Anguilla japonica

  • Kim, Dae-Geun (Gyeongsangnam-do Fisheries Resources Research Institute) ;
  • Kim, Jung-Hyun (Jeju Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Baek, Hea-Ja (Department of Marine Biology, Pukyong National University) ;
  • Kim, Shin-Kwon (Department of Aquaculture Research, National Institute of Fisheries Science) ;
  • Min, Kwan-Sik (Animal Biotechnology, Graduate School of Future Convergence Technology, School of Animal Life Convergence Science, Institute of Genetic Engineering, Hankyong National University) ;
  • Kim, Dae-Jung (Jeju Fisheries Research Institute, National Institute of Fisheries Science)
  • Received : 2020.12.14
  • Accepted : 2021.11.17
  • Published : 2022.01.30
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Abstract

In the present study, we investigated the effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture in vitro and sex steroid hormones in vivo in the Japanese eel Anguilla japonica. To study the in vitro effects of rec-GTHs on estradiol-17β (E2) production in immature ovarian tissues, ovarian tissues were incubated with different doses of rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing hormone (rec-LH). The results revealed that the E2 levels in the rec-FSH (0.1, 0.5, or 1 ㎍/mL)- and rec-LH (0.1 or 0.5 ㎍/mL)-treated groups were significantly higher than those in the female eels from the control group. Furthermore, to investigate the in vivo effects of rec-GTHs on the gonadosomatic index (GSI) and plasma sex steroid hormone levels, the eels were injected intraperitoneally with eel's ringer (control), salmon pituitary extract (SPE; for female eels), human chorionic gonadotropin (hCG; for male eels), rec-FSH, rec-LH, and rec-FSH + rec-LH once a week. The results revealed that except for the SPE and the hCG groups, none of the groups exhibited a significant difference in GSI values. However, in vivo plasma E2 levels increased at the end of 4 weeks after rec-FSH treatment in female eels. Based on these results, it is suggested that rec-GTHs may have a positive effect on sexual maturation in female eels; however, further studies on complementary rec-protein production systems and additional glycosylation of rec-hormones are needed to elucidate hormone bioactivity in vivo and in vitro.

Keywords

Acknowledgement

This work was supported by the National Institute of Fisheries Science project (R2021012).

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