Clinical and Experimental Reproductive Medicine

  • 제49권1호
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  • Pages.40-48
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  • 2022
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Correlations between abnormalities of morphological details and DNA fragmentation in human sperm

  • Nguyen, Hiep Tuyet Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University) ;
  • Dang, Hong Nhan Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University) ;
  • Nguyen, Thai Thanh Thi (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University) ;
  • Nguyen, Trung Van (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University) ;
  • Dang, Thuan Cong (Department of Histology, Embryology, and Pathology, Hue University of Medicine and Pharmacy, Hue University) ;
  • Nguyen, Quoc Huy Vu (Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University) ;
  • Le, Minh Tam (Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University)
  • 투고 : 2021.06.21
  • 심사 : 2021.09.06
  • 발행 : 2022.03.31
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초록

Objective: As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.

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참고문헌

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