Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Starfish smooth muscle relaxing activity of SALMFamide isotype peptide and its analog derived from starfish, Asterias rubens

  • Anastasia Kubarova (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University) ;
  • Hye-Jin Go (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University) ;
  • Hye Young Oh (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University) ;
  • Nam Gyu Park (Department of Biotechnology, College of Fisheries Sciences, Pukyong National University)
  • Received : 2022.09.07
  • Accepted : 2022.10.28
  • Published : 2022.11.30
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Abstract

An organism's physiological processes and behaviors are regulated by neuropeptides and hormone peptides. The first neuropeptide identified from echinoderms is SALMFamide. The two most well-studied SALMFamide neuropeptides are S1 and S2, which possess myoactivity on apical muscle, tube feet, and the cardiac stomach of starfishes. However, neuropeptide candidates identified from SALMFamide's precursor protein sequence have not been investigated. This study aims to compare the bioactivity of SALMFamide neuropeptides from the starfish Asterias rubens using various starfish muscle preparations. In this study, the bioactivity of the L-type SALMFamide neuropeptides from the starfish A. rubens, AYHTGLPFamide (SALMFa-A) and the derivative AYHSALMFamide (SALMFa-B) was investigated. The neuropeptides were applied on Asterias amurensis apical muscle, tube feet, which revealed that the neuropeptides exhibit relaxing activity on apical muscle but no activity on tube feet. The native SALMFa-A peptide had lower relaxing activity on the apical muscle compared to the derivative peptide SALMFa-B. The relaxing activity of two neuropeptides also was compared with those on the apical muscle of Patiria pectinifera, which revealed relaxing activity as well as SALMFamide-S1 and S2 neuropeptides. Moreover, the investigation of SALMFa-A and SALMFa-B peptides' bioactivity on P. pectinifera cardiac stomach muscle also showed slight relaxing activity.

Keywords

Acknowledgement

This work was supported by a Research Grant of Pukyong National University in 2021 (C-D-2021-0773).

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