The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Respiratory Protective Effect of a RML on PM10D-induced Lung Injury Mouse Model

미세먼지 유발 폐기능 손상 동물모델에서 RML의 호흡기 보호 효과

  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Haany University)
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김승형 (대전대학교 동서생명과학연구원) ;
  • 박해진 (대구한의대학교 DHU바이오융복합시험센터)
  • Received : 2022.03.28
  • Accepted : 2022.05.25
  • Published : 2022.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study is aimed to evaluate the protective effects of Rehmanniae Radix, Mori Folium, and Liriopie Tuber mixture (RML) on lung injury of Particulate matter less than 10 um in diameter and diesel exhaust particles (PM10D) mice model. Methods : To investigate the anti-inflammatory activity of RML, PM10D was diluted in aluminum hydroxide (Alum) in 7-week-old male mice and induced by Intra-Nazal-Tracheal (INT) injection method. Animal experiments were divided into 5 groups. Nor (normal mice), CTL (PM10D-induced mice with the administration of distilled water), DEXA (PM10D-induced mice with the administration of 3 mg/kg Dexamethasone), RML 100 (PM10D-induced mice treated with RML 100 mg/kg weight), and RML 200 (PM10D-induced mice treated with RML 200 mg/kg body weight). After 11 days administration, mice were sacrificed and inflammation-related immune cells in broncho-alveolar lavage fluid (BALF) were analyzed. Inflammation-related biomarkers were also analyzed in blood and lungs. Lung tissue was observed through histological examination. Results : In the PM10D induced model, the PML showed decreases in CXCL-1 and IL-17A in BALF. Expression of inflammatory cytokines and cough-related mRNA genes was significantly decreased in serum and lung tissue. The mixture treatment of RML significantly improved the immune related cells in the serum. In addition, histological observations showed a tendency to decrease the severity of lung injury. Conclusions : Overall, these results confirmed the respiratory protective effect of the RML mixture in a model of lung injury induced by air pollution (PM10+DEP), suggesting that it is a potential treatment for respiratory damage.

Keywords

Acknowledgement

본 연구는 2022년도 농촌진흥청 농업과학기술 연구개발사업(과제번호 PJ015272012022)의 지원을 받아 수행된 연구임.

References

  1. Organization for Economic Cooperation and Development (OECD). The economic consequences of outdoor air pollution. 2016 [cited 2019 Nov 26]. Available from:https://www.oecd.org/environment/indicators-modelling-outlooks/Policy-Highlights-Economic-consequences-of-outdoor-air-pollution-web.pdf
  2. Jo HJ, Park SW, Lee HI, Lee SW. Health effects caused by partic-ulate matter and guidelines for health care. Public Health Weekly Report. 2018 ; 11 : 458-62.
  3. Song HW, Ji KY, Kim BK, Yang WK, Han CK, Shin HJ, Park YC, Hwang JS, Kang HS, Kim SH. Respiratory Protective Effect of Salvia plebeia R. Br. Extracts against Ambient Particulate Matter-induced Airway Inflammation. Korean J. Medicinal Crop Sci. 2017 ; 25(5) : 269-81. https://doi.org/10.7783/KJMCS.2017.25.5.269
  4. WHO. WHO Expert Consultation: Available evidence for the fu-ture update of the WHO global air quality guidelines (AQGs). 2016 [cited 2019 Nov 26].Available from:http://www.euro.-who.int/en/health-topics/environment-and-health/air-quality/publications/2016/who-expert-consultation-available-evidence-for-the-future-update-of-the-who-global-air-quality-guidelines- aqgs-2016.
  5. Brandt EB, Kovacic MB, Lee GB, Gibson AM, Acciani TH, Le Cras TD, Ryan PH, Budelsky AL, Hershey GKK. Diesel exhaust particle induction of IL-17A contributes to severe asthma. Journal of Allergy and Clinical Immunology. 2013 ; 132 : 1194-204. https://doi.org/10.1016/j.jaci.2013.06.048
  6. Doeing DC, Solway J. Airway smooth muscle in the pathophysiology and treatment of asthma. Journal of Applied Physiology. 2013 ; 114 : 834-43. https://doi.org/10.1152/japplphysiol.00950.2012
  7. Fuks K, Moebus S, Hertel S, Viehmann A, Nonnemacher M, Dragano N, Mohlenkamp S, Jakobs H, Kessler C, Erbel R, Hoffmann B. Long-term urban particulate air pollution, traffic, noise and arterial blood pressure. Environmental Health Perspectives. 2011 ; 119 : 1706-11. https://doi.org/10.1289/ehp.1103564
  8. Peters S, Reid A, Fritschi L, de Klerk N, Musk AW. Long-term effects of aluminium dust inhalation. Occupational and Environmental Medicine. 2013 ; 70 : 864-8. https://doi.org/10.1136/oemed-2013-101487
  9. Hoffmann B, Weinmayr G, Hennig F, Fuks K, Moebus S, Weimar C, Dragano N, Hermann DM, Kalsch H, Mahabadi AA, Erbel R, Jockel KH. Air quality, stroke, and coronary events: Results of the heinz nixdorf recall study from the ruhr region. Deutsches Arzteblatt International. 2015 ; 112 : 195-201.
  10. Ghio AJ, Smith CB, Madden MC. Diesel exhaust particles and airway inflammation. Current Opinion in Pulmonary Medicine. 2012 ; 18 : 144-50. https://doi.org/10.1097/MCP.0b013e32834f0e2a
  11. Barnes PJ. Cytokines as mediators of chronic asthma. American Journal of Respiratory and Critical Care Medicine. 1994 ; 150 : S42-9. https://doi.org/10.1164/ajrccm/150.5_Pt_2.S42
  12. Bohadana AB, Massin N, Wild P, Toamain JP, Engel S, Goutet P. Symptoms, airway responsiveness, and exposure to dust in beech and oak wood workers. Occupational and Environmental Medicine. 2000 ; 57 : 268-73. https://doi.org/10.1136/oem.57.4.268
  13. Park YB, Rhee CK, Yoon HK, Oh YM, Lim SY, Lee JH, Yoo KH, Ahn JH. COPD clinical practice guideline of the Korean Academy of Tuberculosis and Respiratory Disease: a summary. Tuberc Respir Dis (Seoul). 2018 ; 81(4) : 261-73. https://doi.org/10.4046/trd.2018.0029
  14. Kim TH, Yang WK, Lee SW, Kim SH, Lyu YR, Park YC. Inhibitory Effects of GGX on Lung Injury of Chronic Obstructive Lung Disease (COPD) Mice Model. J Korean Med. 2021 ; 42(3) : 56-71. https://doi.org/10.13048/jkm.21025
  15. Chae BS, Shin TY. Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines. Yakhak Hoeji. 2006 ; 50(1) : 184-90.
  16. Herbology editorial committee of Korean medicine schools. Herbology [Boncho-hak]. Seoul : Young-Lim Press. 2016 : 232, 633.
  17. Kim JS. Effect of Rhemanniae Radix on the Hyperglycemic Mice Induced with Streptozotocin. The Korean Society of Food Science and Nutrition. 2004 ; 33(7) : 1133-8. https://doi.org/10.3746/jkfn.2004.33.7.1133
  18. Bae HK, Seo BI. Inhibitory Activities of Rehmanniae Radix 30% Ethanol Extract on Acute Gastritis and Peptic Ulcers. Kor. J. Herbol. 2019 ; 34(2) : 1-14. https://doi.org/10.6116/KJH.2019.34.2.1
  19. Kwon YD, Song YS. Effects of Rhizoma rehmanniae adminstration on the Innate and Adap tive Responses in the Mouse. j. of Oriental Rehabilitation Medicine. 1998 ; 8(1) : 233-54.
  20. Ju MJ, Kwon JH, Kim HK. Physiological activities of mulberry leaf and fruit extracts with different extraction conditions. Korean J Food Preserv. 2009 ; 16 : 442-8.
  21. Byun SH, Park SM, Kim SC, Cho IJ. Anti-fibrotic Effect of Mori Folium Extract in Hepatic Stellate Cells. Kor J Herbology. 2013 ; 28(4) : 49-55. https://doi.org/10.6116/KJH.2013.28.4.49
  22. Hunyadi A, Veres K, Danko B, Kele Z, Weber E, Hetenyi A, Zupko I, Hsieh T. In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract. Phytother Res. 2013 ; 27(6) : 847-51. https://doi.org/10.1002/ptr.4803
  23. Naowaboot J, Pannangpetch P, Kukonqviriyapan V, Kongyingyoes B, Kukonqviriyapan U. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocininduced chronic diabetic rats. Plant Foods Hum Nutr. 2009 ; 64 : 116-21. https://doi.org/10.1007/s11130-009-0112-5
  24. Freidwald WT, Levy RI, Fredrickson DS. Estimation of concentration of the low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972 ; 18(6) : 499-02. https://doi.org/10.1093/clinchem/18.6.499
  25. Editorial Committee of Oriental Medicine and Herbal Medicine. Herbal medicine. Seoul : Younglimsa. 2005 : 647-48.
  26. National College of Korean Medicine Textbook Compilation Committee. Herbology. Seoul : Youglimsa. 2008 : 644-55.
  27. Kim H. Effect of Maekmoondong-Tang on the immunomodulatory action. Korean Journal of Oriental Physiology & Pathology. 2003 ; 17 : 946-51.
  28. Kim HK, Lee JY, Han HS, Kim YJ, Kim HJ, Kim YS, Kim HM, Ko SG, An HJ, Lee YJ, Park W. Immunomodulatory Effects of Liriope Platyphylla Water Extract on Lipopolysaccharide-Activated Mouse Macrophage. Nutrients. 2012 ; 4(12) : 1887-97. https://doi.org/10.3390/nu4121887
  29. Ahn JH. Effect of Liriopis tuber on Liver function activity. Department of Pharmacy, Catholic University of Daegu. 2000.
  30. Choi SB, Wha JD, Park S. The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway. Life Sci. 2004 ; 75(22) : 2653-64. https://doi.org/10.1016/j.lfs.2004.04.039
  31. Huang TJ, Tsai YC, Chiang SY, Wang GJ, Kuo YC, Chang YC, Wu YY, Wu YC. Anti-viral effect of a compound isolated from Liriope platyphylla against hepatitis B virus in vitro. Virus Res. 2014 ; 192 : 16-24. https://doi.org/10.1016/j.virusres.2014.07.015
  32. Yoshitaka I. Research on the quality distribution of JIS type-II fly ash in Japan. World of Coal Ash. www.flyash.info/2007/67ishikawa.pdf (cited by 2007 May 7).
  33. Lim HB, Kim SH. The effect of crude saponins of Korean red ginseng against airway inflammation and airway hyperresponsiveness induced by diesel exhaust particles in mice. Korean Journal of Medicinal Crop Science. 2009 ; 17 : 90-6.
  34. Fonio, A. Uber ein neues Verfahren der Blutplattchenzahlung. Dtsch. Z. Chir. 1912 ; 117, 176. https://doi.org/10.1007/BF02793644
  35. Grace MS, Baxter M, Dubuis E, Birrell MA, Belvisi MG. Transient receptor potential (TRP) channels in the airway: role in airway disease. Br J Pharmacol. 2014 ; 171 : 2593-607. https://doi.org/10.1111/bph.12538
  36. Wang G, Xu Z, Wang R, Al-Hijji M, Salit J, Strulovici-Barel Y, Tilley AE, Mezey JG, Crystal RG. Genes associated with MUC5AC expression in small airway epithelium of human smokers and nonsmokers. BMC Med Genomics. 2012 ; 5 : 21. https://doi.org/10.1186/1755-8794-5-21
  37. van Manen MJG, Birring SS, Vancheri C, Cottin V, Renzoni EA, Russell AM, Wijsenbeek MS. Cough in idiopathic pulmonary fibrosis. European Respiratory Review. 2016 ; doi:10.1183/16000617.0090-2015.
  38. Park MK, Seong MG, Lee MK. Effects of TRPV1 in formalin-induced nociceptive behavior in theorofacial area of rats. Journal of the Korea Academia-Industrial cooperation Society. 2014 ; 15(1) : 316-22. https://doi.org/10.5762/KAIS.2014.15.1.316
  39. Son HJ, Kim YS, Misaka T, Noh BS, Rhyu MR. Activation of the Chemosensory Ion Channels TRPA1 and TRPV1 by Hydroalcohol Extract of Kalopanax pictus Leaves. Biomol Ther. 2012 ; 20(6) : 550-5. https://doi.org/10.4062/biomolther.2012.20.6.550
  40. Lukacs NW, Hogaboam CM, Kunkel SL. Chemokines and their receptors in chronic pulmonary disease. Curr Drug Targets Inflamm Allergy. 2005 ; 4(3) : 313-7. https://doi.org/10.2174/1568010054022088
  41. Traves SL, Donnelly LE. Th17 cells in airway diseases. Curr Mol Med. 2008 ; 8(5) : 416-26. https://doi.org/10.2174/156652408785160998
  42. Ge Y, Huang M, Yao YM. Biology of Interleukin-17 and Its Pathophysiological Significance in Sepsis. Front. Immunol. 2020 ; doi.org/10.3389/fimmu.2020.01558.
  43. McCreanor J, Cullinan P, Nieuwenhuijsen MJ, Stewart-Evans J, Malliarou E, Jarup L, Harrington R, Svartengren M, Han IK, Ohman-Strickland P, Chung KF, Zhang J. Respiratory effects of exposure to diesel traffic in persons with asthma. N Engl J Med 2007 ; 357(23) : 2348-58. https://doi.org/10.1056/NEJMoa071535