농업과학연구 (Korean Journal of Agricultural Science)

  • 제49권4호
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  • Pages.821-831
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  • 2022
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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Development of an RT-PCR assay and its positive clone for plant quarantine inspection of American plum line pattern virus in Korea

  • Da-Som Lee (Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency) ;
  • Junghwa Lee (Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency) ;
  • Seong-Jin Lee (Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency) ;
  • Seungmo Lim (Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency) ;
  • Jaeyong Chun (Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency)
  • 투고 : 2022.10.10
  • 심사 : 2022.11.03
  • 발행 : 2022.12.01
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초록

American plum line pattern virus (APLPV), a member of the genus Ilarvirus in the family Bromoviridae, is one of the plant quarantine pathogens in Korea. In this study, 15 candidate primer sets were designed and examined to develop a reverse transcription polymerase chain reaction (RT-PCR) assay for plant quarantine inspection of APLPV. Using APLPV-infected and healthy samples, the primer sets were assessed for APLPV detection. To confirm the occurrence of nonspecific reactions, six ilarviruses (Apple mosaic virus, Asparagus virus 2, Blueberry shock virus, Prune dwarf virus, Prunus necrotic ringspot virus, and Tobacco streak virus) and 10 target plants (Prunus mume, P. yedoensis, P. persica, P. armeniaca, P. dulcis, P. tomentosa, P. avium, P. glandulosa, P. salicina, and P. cerasifera) were examined. Finally, two primer sets were selected. These primer sets could generate the expected amplicons even with at least 1 ng of the total RNA template in concentration-dependent amplifications. In addition, a positive clone was developed for use as a positive control in the abovementioned RT-PCR assay.

키워드

과제정보

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (320044-03-1-SB010). We thank Edanz for editing the English text of a draft of this manuscript.

참고문헌

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