Journal of Plant Biotechnology

  • 제49권4호
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  • Pages.271-291
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  • 2022
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Genome-wide identification and expression profiling of the pectin methylesterase gene family in Citrus sinensis (L.) Osbeck

  • Ho Bang Kim (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Chang Jae Oh (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Nam-Hoon Kim (PHYZEN Genomics Institute) ;
  • Cheol Woo Choi (Citrus Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Minju Kim (Citrus Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Sukman Park (Citrus Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Seong Beom Jin (Citrus Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Su-Hyun Yun (Citrus Research Institute, National Institute of Horticultural & Herbal Science) ;
  • Kwan Jeong Song (Major of Horticultural Science, Faculty of Bioscience and Industry, Jeju National University)
  • 투고 : 2022.09.29
  • 심사 : 2022.10.28
  • 발행 : 2022.12.31
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초록

Pectin methylesterase (PME) plays an important role in vegetative and reproductive development and biotic/abiotic stress responses by regulating the degree of methyl-esterification of pectic polysaccharides in the plant cell wall. PMEs are encoded by a large multigene family in higher land plant genomes. In general, the expression of plant PME genes shows tissue- or cell-specific patterns and is induced by endogenous and exogenous stimuli. In this study, we identified PME multigene family members (CsPMEs) from the sweet orange genome and report detailed molecular characterization and expression profiling in different citrus tissues and two fruit developmental stages. We also discussed the possible functional roles of some CsPME genes by comparing them with the known functions of PMEs from other plant species. We identified 48 CsPME genes from the citrus genome. A phylogenetic tree analysis revealed that the identified CsPMEs were divided into two groups/types. Some CsPMEs showed very close phylogenetic relationships with the PMEs whose functions were formerly addressed in Arabidopsis, tomato, and maize. Expression profiling showed that some CsPME genes are highly or specifically expressed in the leaf, root, flower, or fruit. Based on the phylogenetic relationships and gene expression profiling results, we suggest that some CsPMEs could play functional roles in pollen development, pollen tube growth, cross incompatibility, root development, embryo/seed development, stomata movement, and biotic/abiotic stress responses. Our results shed light on the biological roles of individual CsPME isoforms and contribute to the search for genetic variations in citrus genetic resources.

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과제정보

This research was supported by the Cooperative Research Program for Agriculture Science & Technology Development, RDA (Project No. PJ01514103), and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) (Grant No. 322072031HD040), Republic of Korea. The authors appreciate Grace Kim (Yieun Kim) for her illustration work.

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