Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Pseudomonas sp. NIBR-H-19, an Antimicrobial Secondary Metabolite Producer Isolated from the Gut of Korean Native Sea Roach, Ligia exotica

  • Sungmin Hwang (Clean Energy Research Center, Korea Institute of Science and Technology (KIST)) ;
  • Jun Hyeok Yang (Laboratory of Marine Microbiology, Division of Convergence on Marine Science, Korea Maritime and Ocean University) ;
  • Ho Seok Sim (Laboratory of Marine Microbiology, Division of Convergence on Marine Science, Korea Maritime and Ocean University) ;
  • Sung Ho Choi (National Institute of Biological Resources, Environmental Research Complex) ;
  • Byounghee Lee (National Institute of Biological Resources, Environmental Research Complex) ;
  • Woo Young Bang (National Institute of Biological Resources, Environmental Research Complex) ;
  • Ki Hwan Moon (Laboratory of Marine Microbiology, Division of Convergence on Marine Science, Korea Maritime and Ocean University)
  • Received : 2022.08.30
  • Accepted : 2022.10.16
  • Published : 2022.11.28
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Abstract

The need to discover new types of antimicrobial agents has grown since the emergence of antibiotic-resistant pathogens that threaten human health. The world's oceans, comprising complex niches of biodiversity, are a promising environment from which to extract new antibiotics-like compounds. In this study, we newly isolated Pseudomonas sp. NIBR-H-19 from the gut of the sea roach Ligia exotica and present both phenotypes and genomic information consisting of 6,184,379 bp in a single chromosome possessing a total of 5,644 protein-coding genes. Genomic analysis of the isolated species revealed that numerous genes involved in antimicrobial secondary metabolites are predicted throughout the whole genome. Moreover, our analysis showed that among twenty-five pathogenic bacteria, the growth of three pathogens, including Staphylococcus aureus, Streptococcus hominis and Rhodococcus equi, was significantly inhibited by the culture of Pseudomonas sp. NIBR-H-19. The characterization of marine microorganisms with biochemical assays and genomics tools will help uncover the biosynthesis and action mechanism of antimicrobial metabolites for development as antagonistic probiotics against fish pathogens in an aquatic culture system.

Keywords

Acknowledgement

This work was supported by the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the South Korea (NIBR202019103, and NIBR202123102), and the National Research Foundation of Korea (NRF) grants funded by the South Korea government (MSIT) (No. NRF2021R1C1C1004734).

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