Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Fructan Biosynthesis by Yeast Cell Factories

  • Hyunjun Ko (Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Bong Hyun Sung (Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Mi-Jin Kim (Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jung-Hoon Sohn (Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jung-Hoon Bae (Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Received : 2022.07.29
  • Accepted : 2022.08.31
  • Published : 2022.11.28
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Abstract

Fructan is a polysaccharide composed of fructose and can be classified into several types, such as inulin, levan, and fructo-oligosaccharides, based on their linkage patterns and degree of polymerization. Owing to its structural and functional diversity, fructan has been used in various fields including prebiotics, foods and beverages, cosmetics, and pharmaceutical applications. With increasing interest in fructans, efficient and straightforward production methods have been explored. Since the 1990s, yeast cells have been employed as producers of recombinant enzymes for enzymatic conversion of fructans including fructosyltransferases derived from various microbes and plants. More recently, yeast cell factories are highlighted as efficient workhorses for fructan production by direct fermentation. In this review, recent advances and strategies for fructan biosynthesis by yeast cell factories are discussed.

Keywords

Acknowledgement

This work was supported by the Cooperative Research Program for Agriculture Science and Technology Development (PJ0149382021) through the Rural Development Administration of Korea; (ii) Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HP20C0087); (iii) the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MIST) (2021M3E5E6038113), and the Research Initiative Program of KRIBB (Korea Research Institute of Bioscience and Biotechnology).

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