Journal of radiological science and technology (대한방사선기술학회지:방사선기술과학)

Korean Society of Radiological Science (대한방사선과학회)
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Photodynamic Inactivation of Staphylococcus Aureus Based on Dose of Laser Transmission

레이저 투과 선량에 따른 황색포도상구균의 광역학적 비활성화

  • Koo, Bon-Yeoul (Department of Radiology, Vision College of Jeonju) ;
  • Kim, Ji-Won (Department of Radiological Science, Jeonju University)
  • 구본열 (전주비전대학교 방사선학과) ;
  • 김지원 (전주대학교 방사선학과)
  • Received : 2022.02.22
  • Accepted : 2022.03.28
  • Published : 2022.04.30
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Abstract

Staphylococcus aureus is a major pathogen that causes clinical infections in humans and can also cause massively colonized in lesion skin, particularly in atopic dermatitis patients. This study investigated the effects of photodynamic inactivation with radachlorin and diode laser irradiation on the viability of S. aureus in vitro and assessed the effects of the dose of laser transmission. In the PDI group, 5 𝜇L of S. aureus suspension and 5 𝜇L of radachlorin were inoculated in a 55 mm petri dish (63.6 cm2). The samples were placed in a 37° incubator for 30 min and then irradiated with light (660 nm diode laser). After laser irradiation, the cells were stored for 24 h at 37° in an incubator with 5% CO2, and the number of colonies was counted. All CFU/mL of S. aureus were reduced by diode laser in the presence of radachlorin, with a killing rate of 87.9% at an energy dose of 9 J/cm2. This study contribute to treat colonized with S. aureus in atopic dermatitis patients and wound infections by providing information on the optimal dose of laser transmission using PDI to eliminate S. aureus.

Keywords

References

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