Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Evaluation of Liver Toxicity of Neonates Following Intragastric Administration or Intratracheal Instillation of Polyethylene Microplatics to Pregnant Mice

폴리에틸렌 미세플라스틱의 임신 마우스 위내 투여 및 기도 점적에 따른 신생자 간독성 평가

  • Kim, GeunWoo (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University) ;
  • Kim, ChangYul (Department of Toxicity Assessment, Graduate School of Medical and Health Industry, Daegu Catholic University)
  • 김근우 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과) ;
  • 김창열 (대구가톨릭대학교 의료보건산업대학원 화학물질독성평가학과)
  • Received : 2022.02.17
  • Accepted : 2022.03.21
  • Published : 2022.04.30
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Abstract

Background: Current research suggests that humans are exposed to microplastics through consumption of foods and beverages, the airway route, and a variety of other means. Objectives: We evaluated oxidative stress and inflammation from polyethylene microplastics (PE-MPs) in the neonatal liver through intragastric administration or intratracheal instillation in pregnant mice. Methods: PE-MPs were administered from gestational day 9 to postnatal day 7. The intragastric administration group (0.01 mg/mouse/day or 0.1 mg/mouse/day) and intratracheal instillation group (6 ㎍/mouse/day or 60 ㎍/mouse/day) of PE-MPs were administered. After sacrifice, the oxidative stress and inflammation of the neonatal livers were measured. Results: As a result of the oxidative stress caused by PE-MPs in the neonatal livers, glutathione peroxidase decreased in a concentration-dependent manner in the intragastric administration group compared to the control group and intratracheal instillation decreased in high concentration PE-MPs. The catalase level increased at high concentrations of intragastric administration and intratracheal instillation. To confirm the level of inflammation caused by PE-MPs, monocyte chemoattractant protein-1 and tumor necrosis factoralpha were increased compared to the control group except for intratracheal intilation-high concentration PEMPs. The C-reactive protein level was decreased by intragastric administration compared to the control group and intratracheal instillation was increased compared to the control group. Conclusions: Despite the difficulty in comparing the toxic intensity between intragastric administration and intratracheal instillation of PE-MPs, our study revealed that oxidative stress and inflammation were induced in the neonatal liver. However, it is necessary to evaluate the toxic effects of microplastics on various organs as well. Overall, the present study indicates that the evaluation of toxic effects of long-term microplastic exposure, potential of microplastic toxicity on next-generation offspring and toxicity mechanism in human should be considered for further investigations.

Keywords

Acknowledgement

본 연구는 한국화학물질관리협회 화학물질 안전관리 전문인력 양성 사업의 지원을 받아 수행되었음.

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