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Enhancement of polyphenols, flavonoids and antioxidant activities in water extract of mulberry (Morus alba L.) root bark by steam treatment

  • Rahul, Kamidi (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Kweon, HaeYong (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Hyun-Bok (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Ji Hae (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Makwana, Pooja (Central Sericultural Research & Training Institute, Central Silk Board, Ministry of Textiles)
  • Received : 2022.01.05
  • Accepted : 2022.03.23
  • Published : 2022.03.31

Abstract

Different parts of the mulberry plant are described to be potential sources of polyphenolics exhibiting strong antioxidant activity. In this study, we prepared various aqueous extracts of mulberry root bark by subjecting to steam at different temperatures and time intervals (45℃, 15 h; 70℃, 15 h; 95℃, 6 h and 95℃, 15 h) followed by extracting at 80℃ for 1 h. The total polyphenolic content ranged from 66.82-101.20 mg gallic acid equivalent (GE)/g of extract whereas the flavonoids were in the range of 13.03-25.23 mg catechin equivalent (CE)/g of extract. The extracts also exhibited strong antioxidant activities (0.99-1.66 mg trolox equivalent (TE)/g of extract in DDPH assay and 10.65-16.26 mg TE/g of extract in ABTS assay). This study clearly showed an improvement in the antioxidant activity of the water extract of mulberry root bark by the steam treatment, which can be used as a tea or health-promoting materials.

Keywords

Acknowledgement

This study was carried out with the support of 'Research Program for Agricultural Science and Technology Development' (PJ01510701), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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