Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

An In Vitro Study on the Effect of Fermented Citrus junos as a Cosmetic Material

화장품소재로서 유자발효물의 In Vitro 효능 연구

  • 염현숙 (대봉엘에스(주) 제주천연물소재응용연구소) ;
  • 조성미 (P&K 피부임상연구센타(주)) ;
  • 박진오 (대봉엘에스(주) 제주천연물소재응용연구소) ;
  • 이해광 (P&K 피부임상연구센타(주)) ;
  • 이혜자 (대봉엘에스(주) 제주천연물소재응용연구소)
  • Received : 2022.03.15
  • Accepted : 2022.03.30
  • Published : 2022.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the antioxidant activity, wound healing, moisturizing and anti-pollution effects of fermented Citrus junos (FCJ) with Lactobacillus rhamnosus as a cosmetic material. For the anti-oxidative activities, the FCJ showed potent DPPH radical scavenging activities. In addition, FCJ increased cell migration compared to the untreated group in scratch-induced wound healing assay. It was confirmed that the amount of filagrin (FLG), a moisturizing factor, increased in FCJ. FCJ prevented the decrease in cell viability, stimulated by PM10 at in vitro. Based on these results, it is believed that various effects of FCJ can provide a scientific basis for the development of cosmetic raw materials.

본 연구에서는 화장품소재로서 락토바실러스 람노서스(Lactobacillus rhamnosus)로 발효된 유자발효물의 항산화 활성, wound healing, 보습 및 미세먼지 차단 효능을 조사하였다. 항산화 활성을 측정한 결과, 유자발효물에서 우수한 DPPH 라디칼 소거 활성이 있음을 확인하였다. 또한, 유자발효물은 scratch-induced wound healing 실험에서 무처리군 대비 세포 이동을 증가시키는 효과를 나타내었다. 유자발효물에서 보습 인자인 필라그린의 생성량이 증가하는 것을 확인하였다. PM10으로 유도된 세포 자극에 대한 미세먼지 차단 효과를 확인한 결과, 유자발효물의 세포 보호 효과를 확인하였다. 이러한 결과를 바탕으로 유자발효물의 다양한 효과가 화장품원료 개발에 대한 과학적 근거를 제공할 수 있을 것으로 사료된다.

Keywords

References

  1. C. R. Seo, T. H. Ha, J. Y. Moon, J. M. Kim, B. K. Park, J. W. Lee, J. O. Park, and J. H. Shin, Protective effect of cosmetics containing red beet against cigarette smoke-induced oxidative damage in human skin, J. Soc. Cosmet. Sci. Korea, 44(2), 111 (2018). https://doi.org/10.15230/SCSK.2018.44.2.111
  2. T. L. Pan, P. W. Wang, I. A. Aljuffali, C. T. Huang, C. W. Lee, and J. Y. Fang, The impact of urban particulate pollution on skin barrier function and the subsequent drug absorption, Jouranl of Dermatological Science, 78(1), 51 (2015). https://doi.org/10.1016/j.jdermsci.2015.01.011
  3. G. E. Choi, C. H. Lee, and K. Y. Hyun, Anti-oxidant and anti-pollution composition containing the extract of Nypa fruticans wurmb, Saussurea neoserrata, Codium fragile and Enteromorpha compressa, Biomedical Science Letters, 26(3), 157 (2020). https://doi.org/10.15616/BSL.2020.26.3.157
  4. K. Kabashima, T. Honda, F. Ginhoux, and G. Egawa, The immunological anatomy of the skin, Nat. Rev. Immunol., 19(1), 19 (2019). https://doi.org/10.1038/s41577-018-0084-5
  5. D. Y. M. Leung, E. Berdyshev, and E. Goleva, Cutaneous barrier dysfunction in allergic diseases, J. Allergy Clin Immunol., 145(6), 1485 (2020). https://doi.org/10.1016/j.jaci.2020.02.021
  6. K. M. Ahn, B. E. Kim, J. H. Kim, and D. Y. M. Leung, Recent advances in atopic dermatitis, Curr. Opin. Immunol., 66, 14 (2020). https://doi.org/10.1016/j.coi.2020.02.007
  7. B. E. Kim, J. H. Kim, E. Goleva, E. Berdyshev, J. Y. Lee, K. A. Vang, U. H. Lee, S. Y. Han, S. Leung, C. F. Hall, N. R. Kim, I. Bronova, E. J. Lee, H. R. Yang, D. Y. M. Leung, and K. M. Ahn, Particulate matter causes skin barrier dysfunction, JCI Insight, 6(5), 1 (2021).
  8. S. Ejaz, M. Ashraf, M. Nawaz, and C. W. Lim, Total particulate matter and wound healing: An in vivo study with histological insights, Biomedical and enveironmental sciences, 22(4), 278 (2009). https://doi.org/10.1016/S0895-3988(09)60057-X
  9. D. S. Kim, D. H. Kim, M. J. Oh, K. G. Lee, M. C. Kook, and C. S. Park, Antiaging and whitening activities of ethanol extract of Yuza (Citrus junos S ieb ex TANAKA) by-product, J. Soc. Cosmet. Sci. Korea, 36(2), 137 (2010).
  10. S. Tanaka, T. Sato, N. Akimoto, M. Yano, and A. Ito, Prevention of UVB-induced photoinflammation and photoaging by a polymethoxy flavonoid, nobiletin, in human keratinocytes in vivo and in vitro, Biochem Pharmacol., 68(3), 433 (2004). https://doi.org/10.1016/j.bcp.2004.04.006
  11. S. H. Park, B. G. Jeong, W. J. Song, J. Y. Jung, and J. Y. Chun, Enhancement of functional and sensory properties of eastern prickly pear (Opuntia humifusa) by fermentation with yuza peel and guava leaf, Food Bioscience, 41, 100921 (2021). https://doi.org/10.1016/j.fbio.2021.100921
  12. M. S. Blois, Antioxidant determination by the use of a stable free radical, Nature, 181, 1199 (1958). https://doi.org/10.1038/1811199a0
  13. S. Veeraperumal, H. M. Qiu, S. S. Zeng, W. Z. Yao, B. P. Wang, Y. Liu, and K. L. Cheong, Polysaccharides from Gracilaria lemaneiformis promote the HaCaT keratinocytes wound healing by polarised and directional cell migration, Garbohydrate Polymers, 241, 116310 (2020). https://doi.org/10.1016/j.carbpol.2020.116310
  14. H. M. Ko, S. H. Choi, Y. M. Kim, E. J. An, S. H. Lee, K. Kim, H. J. Jung and H. J. Jang, Effect of Rosa laevigata on PM10-induced rnflammatory response of human lung epithelial cells, Evid. Based Complement. Alternat. Med., 2020, 9 (2020).
  15. R Yu, H. Zhang, and B. Guo, Conductive biomaterials as bioactive wound dressing for wound healing and skin tissue engineering, Nano-Micro Letters, 14(1), 1 (2022). https://doi.org/10.1007/s40820-021-00751-y