한국동물생명공학회지 (Journal of Animal Reproduction and Biotechnology)

  • 제37권4호
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  • Pages.213-217
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  • 2022
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  • 2671-4639(pISSN)
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  • 2671-4663(eISSN)
한국동물생명공학회 (The Korean Society of Animal Reproduction and Biotechnology)
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Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer

  • Yeonmi, Lee (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University) ;
  • Eunju, Kang (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University)
  • 투고 : 2022.11.29
  • 심사 : 2022.12.07
  • 발행 : 2022.12.31
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초록

Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans. Some researchers showed partial somatic haploidization, but none observed embryo development. Our study attempted somatic haploidization using the modified somatic nuclear transfer (SCNT) protocol with various combinations of chemicals or proteins in mice. This study induced the proper segregation of somatic homologous chromosomes and full embryo development in vitro. Furthermore, somatic haploid embryos established embryonic stem cells and produced live births. The current review summarizes this recent study on the success of somatic haploidization and provides an overview of other related studies on somatic haploidization.

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과제정보

This work has been supported by the National Research Foundation of Korea (grant nos. NRF-2018R1A2B3001244 and NRF-2021R1I1A1A01049705).

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