Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer

  • Yeonmi, Lee (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University) ;
  • Eunju, Kang (Department of Biomedical Science, College of Life Science and CHA Advanced Research Institute, CHA University)
  • Received : 2022.11.29
  • Accepted : 2022.12.07
  • Published : 2022.12.31
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Abstract

Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans. Some researchers showed partial somatic haploidization, but none observed embryo development. Our study attempted somatic haploidization using the modified somatic nuclear transfer (SCNT) protocol with various combinations of chemicals or proteins in mice. This study induced the proper segregation of somatic homologous chromosomes and full embryo development in vitro. Furthermore, somatic haploid embryos established embryonic stem cells and produced live births. The current review summarizes this recent study on the success of somatic haploidization and provides an overview of other related studies on somatic haploidization.

Keywords

Acknowledgement

This work has been supported by the National Research Foundation of Korea (grant nos. NRF-2018R1A2B3001244 and NRF-2021R1I1A1A01049705).

References

  1. Baltus AE, Menke DB, Hu YC, Goodheart ML, Carpenter AE, de Rooij DG, Page DC. 2006. In germ cells of mouse embryonic ovaries, the decision to enter meiosis precedes premeiotic DNA replication. Nat. Genet. 38:1430-1434. https://doi.org/10.1038/ng1919
  2. Chang CC, Nagy ZP, Abdelmassih R, Yang X, Tian XC. 2004. Nuclear and microtubule dynamics of G2/M somatic nuclei during haploidization in germinal vesicle-stage mouse oocytes. Biol. Reprod. 70:752-758. https://doi.org/10.1095/biolreprod.103.024497
  3. Duan X, Liu J, Dai XX, Liu HL, Cui XS, Kim NH, Wang ZB, Wang Q, Sun SC. 2014. Rho-GTPase effector ROCK phosphorylates cofilin in actin-meditated cytokinesis during mouse oocyte meiosis. Biol. Reprod. 90:37.
  4. Fulka Jr. J, Martinez F, Tepla O, Mrazek M, Tesarik J. 2002. Somatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes. Hum. Reprod. 17:2160-2164. https://doi.org/10.1093/humrep/17.8.2160
  5. Hamazaki N, Kyogoku H, Araki H, Miura F, Horikawa C, Hamada N, Shimamoto S, Hikabe O, Nakashima K, Kitajima TS, Ito T, Leitch HG, Hayashi K. 2021. Reconstitution of the oocyte transcriptional network with transcription factors. Nature 589:264-269. https://doi.org/10.1038/s41586-020-3027-9
  6. Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K. 2016. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature 539:299-303. https://doi.org/10.1038/nature20104
  7. Kim B, So S, Choi J, Kang E, Lee Y. 2022. Efficient method for generating homozygous embryonic stem cells in mice. J. Anim. Reprod. Biotechnol. 37:48-54. https://doi.org/10.12750/JARB.37.1.48
  8. Lan WH, Lin SY, Kao CY, Chang WH, Yeh HY, Chang HY, Chi P, Li HW. 2020. Rad51 facilitates filament assembly of meiosis-specific Dmc1 recombinase. Proc. Natl. Acad. Sci. U. S. A. 117:11257-11264. https://doi.org/10.1073/pnas.1920368117
  9. Lee Y and Kang E. 2021. Hormone induced recipients for embryo transfer in mice. J. Anim. Reprod. Biotechnol. 36:247-252. https://doi.org/10.12750/JARB.36.4.247
  10. Lee Y, Trout A, Marti-Gutierrez N, Kang S, Xie P, Mikhalchenko A, Kim B, Choi J, So S, Han J, Xu J, Koski A, Ma H, Yoon JD, Van Dyken C, Darby H, Liang D, Li Y, Tippner-Hedges R, Xu F, Amato P, Palermo GD, Mitalipov S, Kang E. 2022. Haploidy in somatic cells is induced by mature oocytes in mice. Commun. Biol. 5:95.
  11. Matoba S and Zhang Y. 2018. Somatic cell nuclear transfer reprogramming: mechanisms and applications. Cell Stem Cell 23:471-485.
  12. Nagy ZP and Chang CC. 2007. Artificial gametes. Theriogenology 67:99-104. https://doi.org/10.1016/j.theriogenology.2006.09.013
  13. Nasiri E, Mahmoudi R, Bahadori MH, Amiri I. 2011. The effect of retinoic acid on in vitro maturation and fertilization rate of mouse germinal vesicle stage oocytes. Cell J. 13:19-24.
  14. Palermo GD, Takeuchi T, Rosenwaks Z. 2002. Oocyte-induced haploidization. Reprod. Biomed. Online 4:237-242. https://doi.org/10.1016/S1472-6483(10)61812-3
  15. So S, Karagozlu MZ, Lee Y, Kang E. 2020. Fasudil increases the establishment of somatic cell nuclear transfer embryonic stem cells in mouse. J. Anim. Reprod. Biotechnol. 35:21-27. https://doi.org/10.12750/JARB.35.1.21
  16. Tateno H, Akutsu H, Kamiguchi Y, Latham KE, Yanagimachi R. 2003A. Inability of mature oocytes to create functional haploid genomes from somatic cell nuclei. Fertil. Steril. 79:216-218. https://doi.org/10.1016/S0015-0282(02)04537-5
  17. Tateno H, Latham KE, Yanagimachi R. 2003B. Reproductive semi-cloning respecting biparental origin. A biologically unsound principle. Hum. Reprod. 18:472-473. https://doi.org/10.1093/humrep/deg079
  18. Tesarik J, Nagy ZP, Sousa M, Mendoza C, Abdelmassih R. 2001. Fertilizable oocytes reconstructed from patient's somatic cell nuclei and donor ooplasts. Reprod. Biomed. Online 2:160-164. https://doi.org/10.1016/S1472-6483(10)61950-5
  19. Tesarik J, Mendoza C, Mendoza-Tesarik R. 2021. Human artificial oocytes from patients' somatic cells: past, present and future. Reprod. Fertil. 2:H1-H8. https://doi.org/10.1530/RAF-20-0039
  20. Wang X and Pepling ME. 2021. Regulation of meiotic prophase one in mammalian oocytes. Front. Cell Dev. Biol. 9:667306.
  21. Whitworth KM and Prather RS. 2010. Somatic cell nuclear transfer efficiency: how can it be improved through nuclear remodeling and reprogramming? Mol. Reprod. Dev. 77:1001-1015. https://doi.org/10.1002/mrd.21242
  22. Wilkins AS and Holliday R. 2009. The evolution of meiosis from mitosis. Genetics 181:3-12. https://doi.org/10.1534/genetics.108.099762
  23. Yu CH, Langowitz N, Wu HY, Farhadifar R, Brugues J, Yoo TY, Needleman D. 2014. Measuring microtubule polarity in spindles with second-harmonic generation. Biophys. J. 106:1578-1587. https://doi.org/10.1016/j.bpj.2014.03.009
  24. Zanders SE and Malik HS. 2015. Chromosome segregation: human female meiosis breaks all the rules. Curr. Biol. 25:R654-R656. https://doi.org/10.1016/j.cub.2015.06.054
  25. Zhang PY, Fan Y, Tan T, Yu Y. 2020. Generation of artificial gamete and embryo from stem cells in reproductive medicine. Front. Bioeng. Biotechnol. 8:781.