Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Dihydrodaidzein production from soybean hypocotyl extract by human intestinal bacterium MRG-1

  • Sirirat, Prasertwasu (Metalloenzyme Research Group and Department of Plant Science and Technology, Chung-Ang University) ;
  • Jaehong, Han (Metalloenzyme Research Group and Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2022.11.16
  • Accepted : 2022.12.12
  • Published : 2022.12.31
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Abstract

Phytoestrogenic S-equol production in human gut exclusively depends on the biotransformation of daidzein to dihydrodaidzein (DHD). With a growing demand for the DHD enriched biomaterials, the commercial soybean hypocotyl extract (SHE) was chosen as a substrate for the microbial DHD production by human gut bacterium MRG-1, anaerobic DHD producer. To optimize the production of DHD, anaerobic fermentation conditions, including sterilization time, growth stage of inoculum, and growth media, were investigated. Maximum DHD production (1.2 g/L) was achieved after 48 h incubation when 1% (w/v) of SHE in the 20-min-sterilized Gifu Anaeboic Medium media was inoculated with OD600 0.3-0.4 of MRG-1. This is the first report that crude soy biomaterial, instead of pure compounds, such as daidzin and daidzein, is utilized for the production of the DHD enriched biomaterial.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2021R1A2C2007712).

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