Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Comparative untargeted metabolomic analysis of Korean soybean four varieties (Glycine max (L.) Merr.) based on liquid chromatography mass spectrometry

국내콩 4품종의 LC-MS 기반 비표적대사체 비교평가

  • Eun-Ha, Kim (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Soo-Yun, Park (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Sang-Gu, Lee (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Hyoun-Min, Park (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Oh Suk, Yu (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Yun-Young, Kang (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Myeong Ji, Kim (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Jung-Won, Jung (Biosafety Division, National Institute of Agricultural Sciences) ;
  • Seon-Woo, Oh (Biosafety Division, National Institute of Agricultural Sciences)
  • Received : 2022.11.21
  • Accepted : 2022.12.07
  • Published : 2022.12.31
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Abstract

Soybean is a crop with high-quality of protein and oil, and it is one of the most widely used genetically modified (GM) crops in the world today. In South Korea, Kwangan is the most utilized variety as a parental line for GM soybean development. In this study, untargeted LC-MS metabolomic approaches were used to compare metabolite profiles of Kwangan and three other commercial varieties cultivated in Gunwi and Jeonju in 2020 year. Metabolomic studies revealed that the 4 soybean varieties were distinct based on the partial least squares-discriminant analysis (PLS-DA) score plots; 18 metabolites contributed to variety distinction, including phenylalanine, isoflavones, and fatty acids. All varieties were clearly differentiated by location on the PLS-DA score plot, indicating that the growing environment is also attributable to metabolite variability. In particular, isoflavones and linolenic acid levels in Kwangan were significantly lower and higher, respectively compared to those of the three varieties. It was discussed that it might need to include more diverse conventional varieties as comparators in regard to metabolic characteristics of Kwangan for the assessment of substantial equivalence biogenetically engineered soybeans in a Kwangan-variety background.

콩은 양질의 단백질과 지방산이 풍부하며, 세계적으로 가장 많이 사용되는 형질전환작물(GM) 중 하나이다. 국내에서 GM콩은 주로 광안을 모본으로 하여 개발되고 있는 상황이다. 본 연구에서는 비표적 LC-MS 기반 대사체 분석기술을 이용하여 2020년도에 군위와 전주에서 재배한 광안과 세 일반콩의 대사체 프로파일을 비교분석 하였다. Partial least square-discriminant analysis (PLS-DA) 분석을 통하여 대사체 프로파일들은 품종별로 잘 분리되었으며, 페닐알라닌과 이소플라본, 지방산을 포함하여 18종 물질이 관여하는 것으로 확인하였다. PLS-DA 스코어 플롯에서 콩 4품종은 지역별로 클러스터를 형성하였으며, 이는 재배환경이 대사물질의 변화에 영향을 준 것으로 판단된다. 광안은 다른 품종들에 비하여 이소플라본 함량이 가장 낮았으며, 리놀렌산 함량은 가장 높았다. 광안을 이용하여 개발된 생명공학콩의 실질적동등성 평가의 경우 광안의 대사체 프로파일 특성을 고려한 비교품종 선정 등에 관하여 고찰하였다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 고유기관사업(과제번호: PJ01609702)의 연구비 지원에 의하여 수행되었습니다.

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