Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Studies on the effect of Betula platyphylla extract on human dermal papilla cell proliferation and its mechanism of action

자작나무 추출물에서 보이는 모유두(HDP) 세포 성장 촉진 효과와 작용 메커니즘 연구

  • Seunghyun, Ahn (Department of Applied Chemistry, Dongduk Women's University) ;
  • Jung Yeon, Lee (Department of Applied Chemistry, Dongduk Women's University) ;
  • Eunbi, Hong (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University) ;
  • Jiyun, Kim (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University) ;
  • Won Seok, Jeong (RNS Inc. R&D Center) ;
  • Kown Ki, Moon (VICHE Cosmetic R&D Center) ;
  • CheongTaek, Kim (RNS Inc. R&D Center) ;
  • Jiha, Sung (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University) ;
  • Seyeon, Park (Division of Applied Chemistry and Cosmetic Science, Dongduk Women's University)
  • Received : 2022.09.25
  • Accepted : 2022.10.14
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Betula platyphylla extract includes various materials which showed biological activity such as terpenoids. For this reason, Betula platyphylla extract has been used to alleviate inflammation. In this study, extract of Betula platyphylla was obtained and purified using several solvents and evaluated whether they showed effect on prevention of hair loss. Cell cytotoxicity assay was performed to investigate the effect of extracts on cell proliferation. Western blotting was performed to observe the changes in expression of several related growth factors such as β-catenin, VEGF, IGF1, and cyclin D. Also, 5-α-reductase activity was measured. The ethyl acetate extract was divided into four partial extracts and named as H3-1, H3-2, H3-3, and H3-4. The H3-2 extract showed proliferation activity of human derma papilla cell and increased the protein expression of several related growth factors such as β-catenin, VEGF, IGF1, and cyclin D, comparable to the effect of Ethyl 3,4,5-Trimethoxy Benzoate (ETB)and Lupeol (LPO). Moreover, we found that the fraction H3 was shown to decrease 5-α-reductase activity while ETB and LPO had no significant effect on 5-α-reductase activity.

자작나무(Betula platyphylla) 수피 추출물에는 생리활성을 보일 수 있는 다수의 terpenoids 물질이 포함되어 있고 염증반응을 완화시키는 치료제로 사용되어 왔다. 본 연구는 탈모예방 효과를 보일 수 있는 천연 물질을 확보하기 위해 자작나무를 여러 용매에서 추출 및 분리 정제하여 몇몇 분획을 획득하였다. 이를 사용하여 사람 모낭 모유두세포(HDP)에 대한 세포 독성 및 성장촉진 효과 그리고 β-catenin, VEGF, IGF1, Cyclin D의 발현변화와 5-α-reductase activity에 미치는 영향을 평가하였다. Ethyl acetate를 이용하여 분리한 4개의 분획 중 H3-2 분획은 알려진 유효물질인 ETB, LPO와 동등수준의 모낭 모유두세포(HDP)에 대한 성장 촉진 효과를 보였고 성장에 관여하는 주요인자들의 발현을 높이는 효과를 보였다. 또한, 5-α-reductase activity에 ETB, LPO가 큰 영향을 보이지 못한 반면 최종분획물인 H3은 5-α-reductase activity 감소활성이 있음을 확인할 수 있었다.

Keywords

Acknowledgement

본 논문은 과학기술정보통신부 개인기초연구(과기정통부) (R&D)2019R1F1A1043896 과 중소벤처기업부 지역특화산업육성+(R&D) S2913492의 지원을 받아 수행되었습니다.

References

  1. Cho NK, Kim DH, Sung SH (2014) Simultaneous Determination of Platyphylloside, Aceroside VIII and Betulin in Betula platyphylla bark by HPLC-DAD. Kor J Pharmacogn 45(4): 294-299 
  2. Eom HJ, Kang HR, Choi SU, Kim KH (2017) Cytotoxic Triterpenoids from the Barks of Betula platyphylla var. japonica. Chemistry & Biodiversity 14(4): e1600400. doi:10.1002/cbdv.201600400 
  3. Lukasz Szoka, Valery Isidorov, Jolanta Nazaruk, Marcin Stocki, Leszek Siergiejczyk (2019) Cytotoxicity of Triterpene Seco-Acids form Betula pubescens Buds. Molecules 24(22): 4060-4069. doi:10.3390/molecules24224060 
  4. Jung JY, Jung HA (2010) Effect of Phellinus igniarius Quel Extract on the Anti-inflammatory, Anti allergy, Anti-oxidant, Anti-wrinkle reaction. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology 23(3): 42-65 
  5. Ahn JW, Jang SK, Jo BR, Kim HS, Jeoung EY, Kithenya Hillary, Yoo YM, Joo SS (2022) Promotion effects of steam-dried Betula platyphylla extract on hair regrowth. Korean J Food Sci Technol 54(1): 43-51. doi: 10.9721/KJFST.2022.54.1.43 
  6. Anja Blumeyer, Antonella Tosti, Andrew Messenger, Pascal Reygagne, Veronique Del Marmol, Phyllis I Spuls, Myrto Trakatelli, Andreas Finner, Franklin Kiesewetter, Ralph Trueb, Berthold Rzany, Ulrike Blume-Peytavi (2011) Evidence-based (S3) guideline for the treatment of androgenetic alopecia in women and in men. J. Dtsch Dermatol. Ges. 9(s6) S1-S57. doi: 10.1111/j.1610-0379.2011.07802.x 
  7. A Naito, T Sato, T Matsumoto, K Takeyama, T Yoshino, S Kato, M Ohdera (2008) Dihydrotestosterone inhibits murine hair growth via the androgen receptor. British Journal of Dermatology. 159: 300-305. doi: 10.1111/j.1365-2133.2008.08671.x 
  8. Gupta AK, Foley KA (2014) 5% Minoxidil: treatment for female pattern hair loss. Skin Therapy Lett. 19(6): 5-7 
  9. Jang IW, Ko WS, Yoon HJ (2015) The Latest Trends of Treatment for Alopecia. J Korean Med Ophthalmol Otolaryngol Dermatol 28(4): 12-28. doi: 10.6114/jkood.2015.28.4.012 
  10. Fuchino H, Konishi S, Satoh T, Yagi A, Ssitsu K, Tatsumi T, Tanaka N (1996) Chemical Evaluation of Betula Species in Japan. II. Constituents of Betula platyphylla var. japonica. Chemical and Pharmaceutical Bullein. 44(5): 1033-1038. doi: 10.1248/cpb.44.1033 
  11. Lee CH, Cho YM, Park ES, Shin CS, Lee JY, Jeong HS (2009) In vivo Immune Activity of Sap the White Birch (Betula platyphylla var. japonica). Korean J Food Sci Technol 41(4): 413-416 
  12. Choi SK, Cho NJ, Kim KK (2016) Development and Evaluation of the Herbal Medicine for Hair Growth-promoting Activity. Korean Society for Biotechnology and Bioengineering Journal 31(4): 237-245. doi: 10.7841/ksbbj.2016.31.4.237