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Reference values for selected dry eye tests in normal Beagle dogs: a pilot study

  • Kim, Youngsam (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kang, Seonmi (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Kim, Sunhyo (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Shim, Jaeho (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Go, Seokmin (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Seo, Kangmoon (Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • 투고 : 2021.06.09
  • 심사 : 2021.09.15
  • 발행 : 2022.01.31

초록

Background: Dry eye disease (DED) cannot be diagnosed by a single test because it is a multifactorial disorder of the ocular surface. Although studies on various dry eye tests (DETs) in dogs have been reported, standard criteria have not been established except for the Schirmer tear test 1 (STT-1). Objectives: To establish reference values for DETs in dogs with normal STT-1 values (≥ 15 mm/min) and to analyze the correlation between DETs. Methods: The STT-1 was performed in 10 healthy Beagle dogs (20 eyes). After 20 min, interferometry (IF) for estimating the lipid layer thickness (LLT) of the tear film, tear meniscus height (TMH), non-invasive tear breakup time (NIBUT), and meibomian gland loss rate of the upper eyelid (MGLRU) and lower eyelid (MGLRL), were measured using an ocular surface analyzer. Results: Mean values for STT-1, TMH, and NIBUT were as follows: 21.7 ± 3.4 mm/min, 0.41 ± 0.21 mm, and 19.1 ± 9.5 sec, respectively. The most frequent LLT range, measured by IF, was 30-80 nm (11 eyes), followed by 80 nm (5 eyes) and 80-140 nm (4 eyes). MGLRU ≤ 25% was measured in 11 eyes and 26%-50% in 9 eyes; MGLRL ≤ 25% in 8 eyes and 26%-50% in 12 eyes. Besides positive correlation between TMH and NIBUT (P = 0.038), there were no significant associations between DETs. Conclusions: Data obtained in this study provided normative references that could be useful for diagnosing DED and for further research into correlation between DETs in dogs with DED.

키워드

과제정보

This study was supported by the BK21 FOUR Future Veterinary Medicine Leading Education and Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.

참고문헌

  1. Leiva M, Gimenez TP. Disease of the lacrimal system. In: Maggs DJ, Miller PE, Ofri R, editors. Slatter's Fundamentals of Veterinary Ophthalmology. 6th ed. St. Louis: Elsevier; 2018, p 186-212.
  2. Lemp MA. Report of the National Eye Institute/Industry workshop on clinical trials in dry eyes. CLAO J. 1995;21(4):221-232.
  3. Craig JP, Nichols KK, Akpek EK, Caffery B, Dua HS, Joo CK, et al. TFOS DEWS II definition, and classification report. Ocul Surf. 2017;15(3):276-283. https://doi.org/10.1016/j.jtos.2017.05.008
  4. Korb DR. Survey of preferred tests for diagnosis of the tear film and dry eye. Cornea. 2000;19(4):483-486. https://doi.org/10.1097/00003226-200007000-00016
  5. Saito A, Izumisawa Y, Yamashita K, Kotani T. The effect of third eyelid gland removal on the ocular surface of dogs. Vet Ophthalmol. 2001;4(1):13-18. https://doi.org/10.1046/j.1463-5224.2001.00122.x
  6. Peruccio C. Diagnostic and therapeutic approach to diseases of lacrimal functional unit and ocular surface in dogs and cats. Veterinaria. 2018;32(1):7-26.
  7. Sebbag L, Park SA, Kass PH, Maggs DJ, Attar M, Murphy CJ. Assessment of tear film osmolarity using the TearLabTM osmometer in normal dogs and dogs with keratoconjunctivitis sicca. Vet Ophthalmol. 2017;20(4):357-364. https://doi.org/10.1111/vop.12436
  8. Williams DL, Buckingham A. Measurement of tear osmolarity in the canine eye: a new diagnostic tool for canine keratoconjunctivitis sicca? Res Rev J Vet Sci. 2017;3(2):8-12.
  9. Oria AP, Raposo AC, Araujo NL, Lima FB, Masmali AM. Tear ferning test in healthy dogs. Vet Ophthalmol. 2018;21(4):391-398. https://doi.org/10.1111/vop.12524
  10. Guillon JP, Guillon M. The role of tears in contact lens performance and its measurement. In: Ruben M, Guillon M, editors. Contact Lens Practice. London: Chapman and Hall Medical; 1994, p 453-483.
  11. Maggs DJ. The ophthalmic examination and diagnostic testing. In: Maggs DJ, Miller PE, Ofri R, editors. Slatter's Fundamentals of Veterinary Ophthalmology. 6th ed. St. Louis: Elsevier; 2018, p 18-50.
  12. Lemp MA, Bron AJ, Baudouin C, Benitez Del Castillo JM, Geffen D, Tauber J, et al. Tear osmolarity in the diagnosis and management of dry eye disease. Am J Ophthalmol. 2011;151(5):792-798.e1. https://doi.org/10.1016/j.ajo.2010.10.032
  13. Abelson MB, Ousler GW, Nally LA, Welch D, Krenzer K. Alternative reference values for tear film break up time in normal and dry eye. In: Sullivan DA, Stern ME, Tsubota K, et al., editors. Lacrimal Gland, Tear Film, and Dry Eye Syndrome 3. Advances in Experimental Medicine and Biology. Boston: Springer; 2002, p 1121-1125.
  14. Schiffman RM, Christianson MD, Jacobsen G, Hirsch JD, Reis BL. Reliability and validity of the ocular surface disease index. Arch Ophthalmol. 2000;118(5):615-621. https://doi.org/10.1001/archopht.118.5.615
  15. Pult H, Purslow C, Murphy PJ. The relationship between clinical signs and dry eye symptoms. Eye (Lond). 2011;25(4):502-510. https://doi.org/10.1038/eye.2010.228
  16. Kim RY, Na KS, Park YL, Kim HS. Correlation analysis of tear film lipid layer thickness and ocular surface disease index. J Korean Ophthalmol Soc. 2017;58(7):788-796. https://doi.org/10.3341/jkos.2017.58.7.788
  17. Pult H. Relationships between meibomian gland loss and age, sex, and dry eye. Eye Contact Lens. 2018;44 Suppl 2:S318-S324. https://doi.org/10.1097/ICL.0000000000000467
  18. Wolffsohn JS, Arita R, Chalmers R, Djalilian A, Dogru M, Dumbleton K, et al. TFOS DEWS II diagnostic methodology report. Ocul Surf. 2017;15(3):539-574. https://doi.org/10.1016/j.jtos.2017.05.001
  19. Giannaccare G, Vigo L, Pellegrini M, Sebastiani S, Carones F. Ocular surface workup with automated noninvasive measurements for the diagnosis of meibomian gland dysfunction. Cornea. 2018;37(6):740-745. https://doi.org/10.1097/ICO.0000000000001500
  20. Pult H, Nichols JJ. A review of meibography. Optom Vis Sci. 2012;89(5):E760-E769. https://doi.org/10.1097/opx.0b013e3182512ac1
  21. Hwang H, Jeon HJ, Yow KC, Hwang HS, Chung E. Image-based quantitative analysis of tear film lipid layer thickness for meibomian gland evaluation. Biomed Eng Online. 2017;16(1):135. https://doi.org/10.1186/s12938-017-0426-8
  22. King-Smith PE, Hinel EA, Nichols JJ. Application of a novel interferometric method to investigate the relation between lipid layer thickness and tear film thinning. Invest Ophthalmol Vis Sci. 2010;51(5):2418-2423. https://doi.org/10.1167/iovs.09-4387
  23. Blackie CA, Solomon JD, Scaffidi RC, Greiner JV, Lemp MA, Korb DR. The relationship between dry eye symptoms and lipid layer thickness. Cornea. 2009;28(7):789-794. https://doi.org/10.1097/ICO.0b013e318191b870
  24. Miller PE. Lacrimal system. In: Maggs DJ, Miller PE, Ofri R, editors. Slatter's Fundamentals of Veterinary Ophthalmology. 5th ed. St. Louis: Elsvier; 2013, p 165-183.
  25. Isreb MA, Greiner JV, Korb DR, Glonek T, Mody SS, Finnemore VM, et al. Correlation of lipid layer thickness measurements with fluorescein tear film break-up time and Schirmer's test. Eye (Lond). 2003;17(1):79-83. https://doi.org/10.1038/sj.eye.6700224
  26. Choi SH, Kim NJ, Yung SY, Cha MB. Lipid layer thickness in precorneal tear film. J Korean Ophthalmic Soc. 1997;38(2):195-200.
  27. Craig JP, Nelson JD, Azar DT, Belmonte C, Bron AJ, Chauhan SK, et al. TFOS DEWS II report executive summary. Ocul Surf. 2017;15(4):802-812. https://doi.org/10.1016/j.jtos.2017.08.003
  28. Mainstone JC, Bruce AS, Golding TR. Tear meniscus measurement in the diagnosis of dry eye. Curr Eye Res. 1996;15(6):653-661. https://doi.org/10.3109/02713689609008906
  29. Sebbag L, Allbaugh RA, Wehrman RF, Uhl LK, Ben-Shlomo G, Chen T, et al. Fluorophotometric assessment of tear volume and turnover rate in healthy dogs and cats. J Ocul Pharmacol Ther. 2019;35(9):497-502. https://doi.org/10.1089/jop.2019.0038
  30. Mooi JK, Wang MT, Lim J, Muller A, Craig JP. Minimising instilled volume reduces the impact of fluorescein on clinical measurements of tear film stability. Cont Lens Anterior Eye. 2017;40(3):170-174. https://doi.org/10.1016/j.clae.2017.01.004
  31. Wilson WS, Duncan AJ, Jay JL. Effect of benzalkonium chloride on the stability of the precorneal tear film in rabbit and man. Br J Ophthalmol. 1975;59(11):667-669. https://doi.org/10.1136/bjo.59.11.667
  32. Guillon JP. Non-invasive Tearscope Plus routine for contact lens fitting. Cont Lens Anterior Eye. 1998;21 Suppl 1:S31-S40. https://doi.org/10.1016/S1367-0484(98)80035-0
  33. Vidas Pauk S, Petricek I, Jukic T, Popovic-Suic S, Tomic M, Kalauz M, et al. Noninvasive tear film break-up time assessment using handheld lipid layer examination instrument. Acta Clin Croat. 2019;58(1):63-71.
  34. Lee JS, Salapatek A, Patel P, Soong F. Comparison of non-invasive tear break up times (NIBUT) assessed with video-corneal topography to the standard invasive TBUT as studied in patients after exposure to low humidity environment (LHE). Invest Ophthalmol Vis Sci. 2009;50(13):5-25. https://doi.org/10.1167/iovs.08-1779
  35. Amaechi OU, Osunwoke CM. The relation between invasive and non-invasive tear break-up time in young adults. J Niger Optom Assoc. 2004;11:29-32.
  36. Saito A, Kotani T. Estimation of lacrimal level and testing methods on normal beagles. Vet Ophthalmol. 2001;4(1):7-11. https://doi.org/10.1046/j.1463-5224.2001.00124.x
  37. Moore CP, Wilsman NJ, Nordheim EV, Majors LJ, Collier LL. Density and distribution of canine conjunctival goblet cells. Invest Ophthalmol Vis Sci. 1987;28(12):1925-1932.
  38. Hong J, Sun X, Wei A, Cui X, Li Y, Qian T, et al. Assessment of tear film stability in dry eye with a newly developed keratograph. Cornea. 2013;32(5):716-721. https://doi.org/10.1097/ICO.0b013e3182714425
  39. Pult H, Riede-Pult BH, Nichols JJ. Relation between upper and lower lids' meibomian gland morphology, tear film, and dry eye. Optom Vis Sci. 2012;89(3):E310-E315. https://doi.org/10.1097/opx.0b013e318244e487
  40. Gum GG, Gelatt KN, Esson DW. Physiology of the eye. In: Gelatt KN, editor. Veterinary Ophthalmology. 4th ed. Ames: Blackwell Publishing; 2007, p 149-182.